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[]
["Additional file 13. The primers used in this study."]
[]
[]
[]
["miRNA precursors cloning"]
["Primer_ID","Primer (5'-3')","Comments"]
["seu-miR319a-F","AGAGAGCTTCCTTCAGCCC","pre-miRNA cloning forward primer"]
["seu-miR319a-R","CGATTTGACATATTAAGTGCG","pre-miRNA cloning reverse primer"]
["seu-miR164a-F","TCCACTATAAAATTCAACTTCTCTC","pre-miRNA cloning forward primer"]
["seu-miR164a-R","TTGAATGAAAAGGAAAAAACC","pre-miRNA cloning reverse primer"]
["seu-miR166p-F","CTCTGTCCTTGTCTTTCC","pre-miRNA cloning forward primer"]
["seu-miR166p-R","CAAATCAACTGTGACCTTT","pre-miRNA cloning reverse primer"]
["seu-miR168a-F","CTGATTCGCTTGGTGCAG","pre-miRNA cloning forward primer"]
["seu-miR168a-R","CGGGAAATGGAAATCCACC","pre-miRNA cloning reverse primer"]
["seu-miR398a-F","ATGAGCGAGTTATTTTAGGTGACG","pre-miRNA cloning forward primer"]
["seu-miR398a-R","TTGCCTCCTTTCTAACCTTTGC","pre-miRNA cloning reverse primer"]
["seu-miR399a-F","TAGACATGAACCAAAAGGAGTCACT","pre-miRNA cloning forward primer"]
["seu-miR399a-R","ATCTCCTTTGGCAATATAAATTGTG","pre-miRNA cloning reverse primer"]
["seu-miR1-F","GAGAAGAAAACCCTAACTATGTC","pre-miRNA cloning forward primer"]
["seu-mIR1-R","TAAAAACACGAAAGCCACAAC","pre-miRNA cloning reverse primer"]
["seu-miR2-F","TTAAATACAAGTGAGAACTTACC","pre-miRNA cloning forward primer"]
["seu-miR2-R","CTATCCCAGCAGCAAAAT","pre-miRNA cloning reverse primer"]
["seu-miR3-F","GGAAGAGATAACTTGATTCG","pre-miRNA cloning forward primer"]
["seu-miR3-R","TATCGCTTCACTATTGTAGG","pre-miRNA cloning reverse primer"]
["seu-miR8-F","CATAATCATCACCTTGCACAT","pre-miRNA cloning forward primer"]
["seu-miR8-R","CCTACACGCAAACTCCATC","pre-miRNA cloning reverse primer"]
["seu-miR11-F","TTTGCTGCTAGGATAGGG","pre-miRNA cloning forward primer"]
["seu-miR11-R","CAGGTTTGATCGGGATAG","pre-miRNA cloning reverse primer"]
["seu-miR14-F","TCCTTCAGCCCACTCAT ","pre-miRNA cloning forward primer"]
["seu-miR14-R","TAACTGCAACGATTTGACA","pre-miRNA cloning reverse primer"]
["seu-miR15-F","TAGTTGGTTTATTGATTGACAA","pre-miRNA cloning forward primer"]
["seu-miR15-R","CTATAGTTGGTTTATTGATTGAC","pre-miRNA cloning reverse primer"]
["seu-miR16-F","ACTCATCCTTATTGGTGC","pre-miRNA cloning forward primer"]
["seu-miR16-R","CAAACTGCCAAGAATTGA","pre-miRNA cloning reverse primer"]
["seu-miR19-F","AGGGACTGGCATAAATCC","pre-miRNA cloning forward primer"]
["seu-miR19-R","CTTATATATGAGTTTTAGGGCAA","pre-miRNA cloning reverse primer"]
["seu-miR20-F","GATCGGGTCAAGTGTCAT","pre-miRNA cloning forward primer"]
["seu-miR20-R","TCCTAATTAGGATCCTATCAG","pre-miRNA cloning reverse primer"]
["seu-miR22-F"," CAGTGAGTACTAGGACATGTCTG","pre-miRNA cloning forward primer"]
["seu-miR22-R","AAGGACCAAAAGCAGCATA","pre-miRNA cloning reverse primer"]
["seu-miR23-F","GCCACTGACAGTGGATTG","pre-miRNA cloning forward primer"]
["seu-miR23-R","GATACTAAACCCAAAATAATCAAT ","pre-miRNA cloning reverse primer"]
["seu-miR25-F","TCTCTTCCTAGATATGTATGTGT","pre-miRNA cloning forward primer"]
["seu-miR25-R","CGTTTAGTTTAGTAGATTAATGG","pre-miRNA cloning reverse primer"]
["seu-miR28-F","CCATTTTCCTACAAATCATCC","pre-miRNA cloning forward primer"]
["seu-miR28-R","CACCCTCAAATCGTTTTCT","pre-miRNA cloning reverse primer"]
["seu-miR29-F","ATGGCTTTGACTCGACCTCG","pre-miRNA cloning forward primer"]
["seu-miR29-R","AGTAGACATAAGATGTTCGACGCC","pre-miRNA cloning reverse primer"]
[]
["miRNA qRT-PCR"]
["miRNA_ID","Forward Primer (5'-3')","Stem-loop RT Primer  (5'-3')","Comments"]
["seu-miR160a","GCTGCCTGGCTCCCTGT","GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACtggcat","real-time quantitative RT-PCR"]
["seu-miR319a-3p","CGGCTTGGACTGAAGGGAG","GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACagggag","real-time quantitative RT-PCR"]
["seu-miR399a","CGGCTGCCAAAGGAGATTT","GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACccgggc","real-time quantitative RT-PCR"]
["seu-miR398a-3p","GCGCTGTGTTCTCAGGTCG","GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACcagggg","real-time quantitative RT-PCR"]
["seu-miR394a","CGGCTTGGCATTCTGTCC ","GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACggaggt","real-time quantitative RT-PCR"]
["seu-miR5-3p","GCCGGCTAATAATTGCAACAC","GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACgtcaaa","real-time quantitative RT-PCR"]
["Universal Reverse Primer","GTGCAGGGTCCGAGGT",null,"real-time quantitative RT-PCR"]
["U6 Forward Primer","GGGGACATCCGATAAAATTGG",null,"real-time quantitative RT-PCR"]
["U6 Reverse Primer","ACCATTTCTCGATTTGTGCGT",null,"real-time quantitative RT-PCR"]
[]
["5'- RACE "]
["Target_ID","Primer (5'-3')","Comments"]
["Unigene16755","AGACGAGGGAGATCGTTAGACGTGACATTA","outer reverse primer"]
[null,"GTCGAATTTATGTTCGATGGTG","inner reverse primer"]
["Unigene10818","CGTTATCCTCAAGCAACCACCTCGACTTG","outer reverse primer"]
[null,"GCAACCACCTCGACTTGATC","inner reverse primer"]
["Unigene51030","GTGCAGGCAAATAAATAGCTGATTACGGACC","outer reverse primer"]
[null,"GCTGATTACGGACCAAAACTG","inner reverse primer"]
["Unigene16908","TGACGGACATCTCTTCCACAGCCTGTT","outer reverse primer"]
[null,"CAGCAGTGTGGCCTACTCTTATC","inner reverse primer"]