{"text_format":"3","workbook":"xlsx","n_sheets":6,"sheets":["M1xM2 Up","M1xM2 Down","M2xM3 Up","M2xM3 Down","M3xM4 Up","M3xM4 Down"],"not_rendered":{"defined_names":3},"read":"whole","date_system":"1900"}

{"sheet":"M1xM2 Up","index":0,"n_rows":99,"n_cols":8,"rows_kept":99}
["Additional file 6: Differentially expressed transcripts (DETs) between consecutive megagametophyte developmental stages. Only DETs with positive matches against TAIR are shown"]
["Up-regulated transcripts in M1/M2 (44% were annotated). Annotated transcripts related to developmental process are represented in bold and red color and those related to response to stress and stimulus, in bold and black."]
[]
["Transcript ID","FC","M1","M2","At locus","At name","At annotation","GO term Biological process"]
["isotig87231",99,145.687,0,"AT5G20650.1","COPT5","Copper transporter 5 "]
["isotig19259",99,28.112,0,"AT2G20750.1","EXPB1","Expansin-B1 ","developmental process"]
["isotig73571",99,23.466,0,"AT1G29520.1",null,"AWPM-19-like family protein "]
["isotig94184",99,14.492,0,"AT1G65930.1","CICDH","Cytosolic isocitrate dehydrogenase ","response to stress"]
["isotig103047",99,14.175,0,"AT4G25070.1",null,"unknown protein"]
["PUT-63709",99,13.415,0,"AT5G63140.1","PAP29","Probable inactive purple acid phosphatase 29 "]
["isotig66335",99,13.358,0,"AT2G20750.1","EXPB1","Expansin-B1 ","developmental process"]
["isotig64670",99,12.65,0,"AT1G55910.1","ZIP11","Zinc transporter 11 "]
["isotig114778",99,11.478,0,"AT3G57300.1","INO80","DNA helicase INO80 ","response to stress"]
["PUT-44838",99,11.048,0,"AT1G04290.1",null,"Thioesterase superfamily protein "]
["isotig76162",99,11.029,0,"AT3G43120.1",null,"SAUR-like auxin-responsive protein family ","response to hormone"]
["isotig45257",99,10.123,0,"AT1G55910.1","ZIP11","Zinc transporter 11 "]
["isotig70310",4.26475149906374,13.283,0.691,"AT1G55910.1","ZIP11","Zinc transporter 11 "]
["isotig12101",3.90260765301833,16.481,1.102,"AT2G20750.1","EXPB1","Expansin-B1 ","developmental process"]
["isotig61412",3.75744205655647,16.675,1.233,"AT1G55910.1","ZIP11","Zinc transporter 11 "]
["isotig80596",3.68019195698701,10.896,0.85,"AT3G04620.1",null,"Alba DNA/RNA-binding protein "]
["PUT-2500",3.55451696314381,40.136,3.416,"AT2G16850.1","PIP2-8","Probable aquaporin PIP2-8 "]
["isotig64894",3.48732749759903,11.697,1.043,"AT3G16300.1",null,"CASP-like protein 3A1 "]
["isotig14900",3.46372870653983,14.122,1.28,"AT1G10660.2",null,"unknown protein"]
["isotig75521",3.34329247519292,16.259,1.602,"AT3G21510.1","AHP1","Histidine-containing phosphotransfer protein 1 ","response to hormone"]
["isotig68162",3.34309593969655,13.182,1.299,"AT4G35530.1",null,"phosphatidylinositolglycan-related "]
["isotig51059",3.18246775318388,12.592,1.387,"AT5G59320.1","LTP3","Non-specific lipid-transfer protein 3 ","response to stress"]
["isotig28367",3.13944297327801,14.143,1.605,"AT2G42650.1",null,"Ribosomal protein L1p/L10e family "]
["isotig103619",3.09506754248276,11.698,1.369,"AT2G33220.1",null,"NADH dehydrogenase "]
["isotig115105",3.09485872427085,30.313,3.548,"AT2G14620.1","XTH10","Probable xyloglucan endotransglucosylase/hydrolase protein 10 "]
["isotig51461",3.09454477048511,13.274,1.554,"AT2G02850.1","ARPN","Basic blue protein ","developmental process"]
["isotig71492",3.0584763865368,29.958,3.596,"AT3G42725.1",null,"Putative membrane lipoprotein "]
["isotig48078",3.03364870755477,15.182,1.854,"AT1G22160.1",null,"Protein of unknown function (DUF581) "]
["isotig37804",3.02089101476085,26.363,3.248,"AT4G09960.3","AGL11","Agamous-like MADS-box protein AGL11 ","developmental process"]
["isotig48579",2.96948132489967,14.921,1.905,"AT1G52140.1",null,"unknown protein"]
["isotig25766",2.90211289829669,32.106,4.295,"AT3G46030.1","HTB11","Histone H2B.7 "]
["isotig24641",2.82536799588016,17.408,2.456,"AT1G63750.1",null,"Disease resistance protein (TIR-NBS-LRR class) family ","defense response"]
["isotig89014",2.79552331511266,13.358,1.924,"AT2G17695.1",null,"UPF0548 protein At2g17695 "]
["isotig67570",2.67999120087403,11.734,1.831,"AT1G78930.1",null,"Mitochondrial transcription termination factor family protein "]
["isotig68449",2.67974508701357,12.251,1.912,"AT4G17520.1",null,"Hyaluronan / mRNA binding family "]
["isotig30892",2.67967197899704,10.104,1.577,"AT3G27060.1","TSO2","Ribonucleoside-diphosphate reductase small chain C ","developmental process; response to stimulus"]
["isotig36367",2.63081700474177,21.065,3.401,"AT2G17695.1",null,"UPF0548 protein At2g17695 "]
["isotig30708",2.61848721347725,12.19,1.985,"AT5G11540.1","GULLO3","L-gulonolactone oxidase 3 "]
["isotig47034",2.60955255319485,23.491,3.849,"AT3G56500.1",null,"serine-rich protein-related "]
["isotig28370",2.58049290472753,16.114,2.694,"AT5G09590.1","HSP70-10","Heat shock 70 kDa protein 10, mitochondrial ","response to stress"]
["isotig18115",2.55462679387236,14.259,2.427,"AT5G01850.1",null,"Protein kinase superfamily protein "]
["isotig23809",2.55446341162183,10.063,1.713,"AT3G61860.1","RS31","Serine/arginine-rich splicing factor RS31 "]
["PUT-71189",2.52785587633921,88.664,15.374,"AT1G13950.1","ELF5A-1","Eukaryotic translation initiation factor 5A-1 ","developmental process"]
["isotig69340",2.50999883843995,11.381,1.998,"AT3G08740.1",null,"elongation factor P (EF-P) family protein "]
["PUT-17171",2.48726658332846,68.11,12.147,"AT4G03210.1","XTH9","Xyloglucan endotransglucosylase/hydrolase protein 9 "]
["isotig111731",2.48722090781979,53.911,9.615,"AT2G39270.1",null,"Probable adenylate kinase 6, chloroplastic "]
["isotig43119",2.41699029185897,12.075,2.261,"AT3G20660.1","OCT4","Organic cation/carnitine transporter 4 "]
["isotig50474",2.41694702958261,32.005,5.993,"AT3G03590.1",null,"SWIB/MDM2 domain superfamily protein "]
["isotig50511",2.41691870314546,15.348,2.874,"AT1G68260.1",null,"Thioesterase superfamily protein "]
["isotig66436",2.41681370712937,12.191,2.283,"AT1G50670.1",null,"OTU-like cysteine protease family protein "]
["isotig92654",2.41681050557412,10.856,2.033,"AT3G23920.1","BAM1","Beta-amylase 1, chloroplastic "]
["isotig89466",2.4168007882814,10.37,1.942,"AT3G02468.1","CPuORF9","conserved peptide upstream open reading frame 9 "]
["isotig104093",2.41678164018145,14.898,2.79,"AT5G59320.1","LTP3","Non-specific lipid-transfer protein 3 ","response to stress"]
["isotig113191",2.41665541471239,14.87,2.785,"AT1G69550.1",null,"disease resistance protein (TIR-NBS-LRR class) ","response to stress"]
["PUT-71747",2.39258266252672,395.739,75.365,"AT1G48830.1","RPS7A","40S ribosomal protein S7-1 "]
["isotig21741",2.38454189465646,22.344,4.279,"AT1G01490.1",null,"Heavy metal transport/detoxification superfamily protein "]
["isotig19890",2.37499572104195,12.377,2.386,"AT3G11520.1","CYCB1-3","Cyclin-B1-3 "]
["isotig61752",2.35817962957565,10.921,2.13,"AT4G36850.1",null,"PQ-loop repeat family protein / transmembrane family protein "]
["isotig80888",2.35775795856535,21.892,4.271,"ATMG00580.1","NAD4","NADH dehydrogenase subunit 4 "]
["isotig58779",2.32535167276566,21.095,4.209,"AT5G23070.1",null,"Thymidine kinase "]
["isotig70562",2.31730130166586,20.858,4.185,"AT4G22530.1",null,"S-adenosyl-L-methionine-dependent methyltransferases superfamily protein "]
["isotig23259",2.31704050915578,10.903,2.188,"AT1G65000.1",null,"unknown protein"]
["isotig59168",2.30435047402455,13.297,2.692,"AT5G55950.1",null,"Nucleotide/sugar transporter family protein "]
["isotig46628",2.30424695391886,21.085,4.269,"AT2G36870.1","XTH32","Probable xyloglucan endotransglucosylase/hydrolase protein 32 "]
["isotig24977",2.3041682783504,11.868,2.403,"AT2G39390.1","RPL35B","60S ribosomal protein L35-2 "]
["isotig41365",2.30151101447463,19.014,3.857,"AT4G14385.1",null,"FUNCTIONS IN: molecular_function unknown"]
["isotig38396",2.28251427251366,11.915,2.449,"AT1G68780.1",null,"RNI-like superfamily protein "]
["isotig108248",2.26483970912375,17.912,3.727,"AT1G04820.1","TUBA4","Tubulin alpha-4 chain "]
["isotig77004",2.26482213225944,31.008,6.452,"AT3G27200.1",null,"Cupredoxin superfamily protein "]
["isotig66237",2.26460008656453,10.879,2.264,"AT3G06590.1","BHLH148","Transcription factor bHLH148 "]
["isotig63121",2.21040339937936,13.102,2.831,"AT1G56070.1","LOS1","Ribosomal protein S5/Elongation factor G/III/V family protein ","response to stress"]
["isotig49686",2.21038187557784,19.817,4.282,"AT5G65220.1","RPL29","50S ribosomal protein L29, chloroplastic "]
["isotig72661",2.21028933839399,20.931,4.523,"AT4G02280.1","SUS3","Sucrose synthase 3 "]
["isotig64331",2.19003110101226,25.759,5.645,"AT1G13130.1",null,"Cellulase (glycosyl hydrolase family 5) protein "]
["isotig32064",2.18249052198567,14.299,3.15,"AT5G67070.1","RALFL34","Protein RALF-like 34 "]
["isotig70862",2.16536902266426,15.808,3.524,"AT1G16170.1",null,"unknown protein"]
["isotig82844",2.1652939039023,19.75,4.403,"AT1G56220.1",null,"Dormancy/auxin associated family protein "]
["isotig61599",2.16521561561095,37.466,8.353,"AT2G40010.1","RPP0A","60S acidic ribosomal protein P0-1 "]
["isotig95598",2.09516680147468,13.707,3.208,"AT1G08880.1","HTA5","Probable histone H2AXa "]
["isotig23187",2.0950027052418,19.52,4.569,"AT1G32370.2","TOM2B","Tobamovirus multiplication protein 2B "]
["PUT-17949",2.0949266464556,13.081,3.062,"AT1G55060.1","UBQ12","Polyubiquitin 12 "]
["isotig19314",2.09491262553876,23.59,5.522,"AT3G42790.1","AL3","PHD finger protein ALFIN-LIKE 3 "]
["PUT-294",2.09488339175412,13.888,3.251,"ATCG00500.1","ACCD","acetyl-CoA carboxylase carboxyl transferase subunit beta "]
["PUT-47304",2.09486026155087,26.054,6.099,"AT1G04820.1","TUBA4","Tubulin alpha-4 chain "]
["isotig75416",2.09471456473925,10.072,2.358,"AT1G24020.1","MLP423","MLP-like protein 423 ","defense response"]
["isotig60715",2.0583367261838,16.402,3.938,"AT5G63890.1","HISN8","Histidinol dehydrogenase, chloroplastic ","developmental process; response to stimulus"]
["isotig24381",2.04568092582405,10.235,2.479,"AT5G46280.1","MCM3","DNA replication licensing factor MCM3 "]
["isotig50087",2.04253954496453,18.172,4.411,"AT1G25220.1","ASB1","Anthranilate synthase beta subunit 1, chloroplastic ","developmental process; response to stimulus"]
["isotig62615",2.04252934215703,13.047,3.167,"AT5G01410.1","PDX13","Pyridoxal 5'-phosphate synthase subunit PDX1.3 ","response to stress"]
["isotig70560",2.03350992238105,17.117,4.181,"AT4G25740.1","RPS10A","40S ribosomal protein S10-1 "]
["isotig49153",2.02084957268478,12.406,3.057,"AT1G57540.1",null,"unknown protein"]
["isotig30484",2.02082729495267,15.21,3.748,"AT3G22220.1",null,"hAT transposon superfamily "]
["isotig65803",2.02077350565077,11.192,2.758,"AT5G25080.1",null,"Sas10/Utp3/C1D family "]
["isotig27936",2.00185730838108,11.659,2.911,"AT2G46410.1","CPC","Transcription factor CPC ","developmental process; response to stimulus"]
["isotig13326",2.00179534178451,10.453,2.61,"AT2G18050.1","HIS1-3","histone H1-3 ","response to stress"]

{"sheet":"M1xM2 Down","index":1,"n_rows":38,"n_cols":8,"rows_kept":38}
["Additional file 6: Differentially expressed transcripts (DETs) between consecutive megagametophyte developmental stages. Only DETs with positive matches against TAIR are shown"]
["Down-regulated transcripts in M1/M2 (22% were annotated). Annotated transcripts related to response to stress and stimulus in bold. "]
[]
["Transcript ID","FC","M1","M2","At locus","At name","At annotation","GO term Biological process"]
["isotig115185",-99,0,14.329,"AT5G37770.1","CML24","Calcium-binding protein CML24 ","developmental process; response stimulus "]
["PUT-52720",-99,0,13.178,"AT2G03880.1","PCMP-H44","Pentatricopeptide repeat-containing protein At2g03880, mitochondrial "]
["isotig115332",-99,0,11.014,"AT1G15030.1",null,"Protein of unknown function (DUF789) "]
["isotig21724",-99,0,10.035,"AT1G48090.1",null,"calcium-dependent lipid-binding family protein "]
["Cl#671_759_2",-4.00939948740216,5.192,83.615,"AT3G45310.1",null,"Thiol protease aleurain-like "]
["isotig67633",-3.81230225225084,1.31,18.403,"AT1G14520.1","MIOX1","Inositol oxygenase 1 "]
["isotig72287",-3.71197121469934,0.786,10.3,"AT1G64340.1",null,"unknown protein"]
["isotig76617",-3.71193227017818,0.856,11.217,"AT1G19210.1","ERF017","Ethylene-responsive transcription factor ERF017 "]
["isotig61783",-3.66052980035262,0.905,11.444,"AT5G13700.1","PAO1","Polyamine oxidase 1 "]
["isotig80194",-3.48975685265855,0.903,10.144,"AT1G01490.1",null,"Heavy metal transport/detoxification superfamily protein "]
["isotig89992",-3.38620884621963,6.255,65.4,"AT2G44480.1","BGLU17","Beta-glucosidase 17 "]
["isotig66681",-3.22730309082585,1.854,17.363,"AT1G80920.1","ATJ8","Chaperone protein dnaJ 8, chloroplastic "]
["isotig94484",-2.99230733569368,2.233,17.769,"AT5G48540.1","CRRSP55","Cysteine-rich repeat secretory protein 55 "]
["isotig07081",-2.71204690089299,1.895,12.417,"AT5G59720.1","HSP18.1","18.1 kDa class I heat shock protein ","response to stress"]
["isotig36483",-2.66001694311318,3.558,22.488,"AT1G07900.1","LBD1","LOB domain-containing protein 1 "]
["isotig90068",-2.60556702474706,2.074,12.623,"AT5G06950.1","TGA2","Transcription factor TGA2 ","response to stress"]
["isotig75537",-2.58319466658618,4.219,25.283,"AT4G10250.1","HSP22.0","22.0 kDa heat shock protein ","response to stress"]
["PUT-21251",-2.49000818380616,3.043,17.095,"AT5G42080.1","DRP1A","Dynamin-related protein 1A ","developmental process"]
["isotig64602",-2.45525217480562,3.834,21.026,"AT1G80820.1","CCR2","Cinnamoyl-CoA reductase 2 ","response to stress"]
["isotig64491",-2.36432102375054,2.173,11.189,"AT1G75590.1",null,"SAUR-like auxin-responsive protein family ","response to stimulus"]
["Cl#2257_713_2",-2.23852712728034,27.634,130.409,"AT2G46620.1",null,"P-loop containing nucleoside triphosphate hydrolases superfamily protein "]
["isotig86559",-2.2271381329871,3.035,14.21,"AT3G58450.1",null,"Adenine nucleotide alpha hydrolases-like superfamily protein ","response to stress"]
["isotig96551",-2.22709283105974,2.342,10.965,"AT2G21820.1",null,"unknown protein"]
["PUT-38879",-2.22706737120036,9.111,42.656,"AT4G27960.1","UBC9","SUMO-conjugating enzyme UBC9 "]
["isotig04840",-2.22696404861664,4.141,19.386,"AT5G59720.1","HSP18.1","18.1 kDa class I heat shock protein ","response to stress"]
["isotig76074",-2.18529055036822,6.029,27.421,"AT2G46600.1","KIC","Calcium-binding protein KIC "]
["isotig28737",-2.15286882514651,3.082,13.706,"AT3G55570.1",null,"unknown protein"]
["isotig33471",-2.0928447282702,2.896,12.354,"AT3G17860.1","TIFY6B","Protein TIFY 6B ","response to stimulus"]
["isotig83964",-2.0751207231961,3.798,16.004,"AT1G29680.1",null,"Protein of unknown function (DUF1264) "]
["isotig106692",-2.07501929027623,10.37,43.694,"AT5G25940.1",null,"early nodulin-related "]
["isotig109277",-2.07487220922228,4.116,17.341,"AT1G23410.1","RPS27AA","Ubiquitin-40S ribosomal protein S27a-1 "]
["isotig110579",-2.02066837213784,6.913,28.051,"AT1G03890.1","CRD","12S seed storage protein CRD "]
["Cl#110_881_2",-2.02050122689655,8.21,33.31,"AT5G44190.1","GLK2","Transcription activator GLK2 ","developmental process"]
["isotig19284",-2.00451580982047,3.429,13.759,"AT5G42800.1","DFRA","Dihydroflavonol-4-reductase "]

{"sheet":"M2xM3 Up","index":2,"n_rows":22,"n_cols":8,"rows_kept":22}
["Additional file 6: Differentially expressed transcripts (DETs) between consecutive megagametophyte developmental stages. Only DETs with positive matches against TAIR are shown"]
["Up-regulated transcripts in M2/M3 (22% were annotated). Annotated transcripts related to developmental process were represented in bold and red color and those related to response to stress and stimulus in bold and black. "]
[]
["Transcript ID","FC","M2","M3","At locus","At name","At annotation","GO term Biological process"]
["PUT-21251",99,17.095,0,"AT5G42080.1","DRP1A","Dynamin-related protein 1A "]
["isotig115185",99,14.329,0,"AT5G37770.1","CML24","Calcium-binding protein CML24 ","developmental process; response to stimulus"]
["PUT-59271",99,13.99,0,"AT1G67090.1","RBCS-1A","Ribulose bisphosphate carboxylase small chain 1A, chloroplastic ","response to stress"]
["isotig114285",99,12.669,0,"AT1G09330.1","ECH","Golgi apparatus membrane protein-like protein ECHIDNA "]
["isotig115332",99,11.014,0,"AT1G15030.1",null,"Protein of unknown function (DUF789) "]
["isotig101448",99,10.092,0,"AT1G56170.1","NFYC2","Nuclear transcription factor Y subunit C-2 "]
["isotig76617",3.77744222363758,11.217,0.818,"AT1G19210.1","ERF017","Ethylene-responsive transcription factor ERF017 ","response to stimulus"]
["isotig100066",3.14009328171991,10.817,1.227,"AT5G14040.1","MPT3","Mitochondrial phosphate carrier protein 3, mitochondrial "]
["isotig107658",2.87736900842194,25.564,3.479,"AT4G36800.1","RCE1","NEDD8-conjugating enzyme Ubc12 ","embryo development; response to auxin"]
["isotig51743",2.70768165897429,16.077,2.461,"AT4G23330.2",null,"BEST Arabidopsis thaliana protein match is: eukaryotic translation initiation factor 3A (TAIR:AT4G11420.1)"]
["118-2-3-A08.r.1",2.55582039081372,13.624,2.317,"AT5G53970.1",null,"Probable aminotransferase TAT2 "]
["isotig92164",2.42996612796135,11.074,2.055,"AT1G79810.1","PEX2","Peroxisome biogenesis protein 2 ","developmental process; response to stimulus"]
["isotig101851",2.29261285164038,12.812,2.615,"AT2G43810.1","LSM6B","Sm-like protein LSM36B "]
["isotig63168",2.17241804162148,10.715,2.377,"AT3G60340.1",null,"alpha/beta-Hydrolases superfamily protein "]
["isotig81766",2.08602048263962,11.247,2.649,"AT3G16770.1","RAP2-3","Ethylene-responsive transcription factor RAP2-3 ","response to stimulus"]
["isotig04840",2.07030002734104,19.386,4.616,"AT5G59720.1","HSP18.1","18.1 kDa class I heat shock protein ","response to stress"]
["isotig61983",2.0294340885799,10.745,2.632,"AT3G59310.1",null,"Eukaryotic protein of unknown function (DUF914) "]
["isotig47025",2.01024138341162,16.823,4.176,"AT2G18540.1",null,"RmlC-like cupins superfamily protein "]

{"sheet":"M2xM3 Down","index":3,"n_rows":29,"n_cols":8,"rows_kept":29}
["Additional file 6: Differentially expressed transcripts (DETs) between consecutive megagametophyte developmental stages. Only DETs with positive matches against TAIR are shown"]
["Down-regulated transcripts in M2/M3 (35% were annotated). Annotated transcripts related to response to stress and stimulus in bold. "]
[]
["Transcript ID","FC","M2","M3","At locus","At name","At annotation","GO term Biological process"]
["PUT-42376",-99,0,10.178,"AT1G59870.1","ABCG36","ABC transporter G family member 36 ","response to stimulus "]
["isotig24154",-5.03039636199122,0.703,22.975,"AT4G11650.1","OSM34","Osmotin-like protein OSM34 ","response to stress"]
["isotig56662",-4.47746876246999,1.391,30.987,"AT4G16280.2","FCA","RNA binding","developmental process"]
["isotig84059",-3.61464367910151,0.89,10.902,"AT5G42500.1","DIR2","Dirigent protein 2 ","response to stimulus "]
["isotig97271",-3.61389974499022,1.127,13.798,"AT1G77450.1","anac032","NAC domain containing protein 32 ","developmental process"]
["isotig32032",-3.02969136748697,2.104,17.182,"AT3G48990.1","AAE3","Oxalate--CoA ligase ","response to stimulus "]
["118-2-6-C10.r.1",-2.83690196069036,1.512,10.803,"AT1G67785.1",null,"unknown protein"]
["isotig83170",-2.83689445298654,1.761,12.582,"AT1G80160.1",null,"Lactoylglutathione lyase / glyoxalase I family protein "]
["isotig79448",-2.76649917694701,2.507,17.059,"AT3G10120.1",null,"unknown protein"]
["isotig48078",-2.76642705723813,1.854,12.615,"AT1G22160.1",null,"Protein of unknown function (DUF581) "]
["isotig77638",-2.73028552893039,1.656,10.989,"AT2G23790.1",null,"Calcium uniporter protein 2, mitochondrial "]
["isotig53273",-2.69214514277744,2.697,17.43,"AT4G11050.1","AtGH9C3","Endoglucanase 19 "]
["isotig82703",-2.61444456967465,1.776,10.876,"AT1G07400.1","HSP17.8","17.8 kDa class I heat shock protein ","response to stress"]
["isotig89014",-2.4889841848583,1.924,10.801,"AT2G17695.1",null,"UPF0548 protein At2g17695 "]
["isotig86671",-2.40791267820321,4.618,24.508,"AT3G58450.1",null,"Adenine nucleotide alpha hydrolases-like superfamily protein ","response to stimulus "]
["PUT-2500",-2.35152373460761,3.416,17.434,"AT2G16850.1","PIP2-8","Probable aquaporin PIP2-8 "]
["isotig116368",-2.35143313407553,4.472,22.822,"AT1G55060.1","UBQ12","Polyubiquitin 12 "]
["PUT-294",-2.35127146080546,3.251,16.589,"ATCG00500.1","ACCD","acetyl-CoA carboxylase carboxyl transferase subunit beta "]
["isotig72661",-2.35122881309658,4.523,23.079,"AT4G02280.1","SUS3","Sucrose synthase 3 "]
["isotig95853",-2.14513598942673,3.222,14.252,"AT5G63500.1",null,"Protein of unknown function (DUF 3339) "]
["isotig51249",-2.11692517093945,2.911,12.627,"AT1G52890.1","NAC019","NAC domain-containing protein 19 ","developmental process; response to stimulus"]
["PUT-17171",-2.11690174979032,12.147,52.689,"AT4G03210.1","XTH9","Xyloglucan endotransglucosylase/hydrolase protein 9 "]
["isotig113009",-2.02954338717595,2.743,11.199,"AT1G69550.1",null,"disease resistance protein (TIR-NBS-LRR class) ","response to stimulus"]
["isotig89593",-2.02930783233478,2.948,12.034,"AT3G16510.1",null,"Calcium-dependent lipid-binding (CaLB domain) family protein "]
["PUT-73734",-2.02926851994429,2.952,12.05,"AT3G17800.1",null,"Protein of unknown function (DUF760) ","response to stimulus "]

{"sheet":"M3xM4 Up","index":4,"n_rows":29,"n_cols":8,"rows_kept":29}
["Additional file 6: Differentially expressed transcripts (DETs) between consecutive megagametophyte developmental stages. Only DETs with positive matches against TAIR are shown"]
["Up-regulated transcripts in M3/M4 (22% were annotated). Annotated transcripts related to response to stress and stimulus in bold. "]
[]
["Transcript ID","FC","M3","M4","At locus","At name","At annotation","GO term Biological process"]
["isotig116368",-99,22.822,0,"AT1G55060.1","UBQ12","Polyubiquitin 12 "]
["PUT-51924",-99,15.433,0,"AT1G54290.1",null,"Protein translation factor SUI1 homolog 2 "]
["isotig43193",-99,11.463,0,"AT1G70480.1",null,"Domain of unknown function (DUF220) "]
["isotig77638",-99,10.989,0,"AT2G23790.1",null,"Calcium uniporter protein 2, mitochondrial "]
["PUT-42376",-99,10.178,0,"AT1G59870.1","ABCG36","ABC transporter G family member 36 ","response to stress"]
["isotig83326",-3.73878387039719,13.497,1.011,"AT2G18196.1",null,"Heavy metal transport/detoxification superfamily protein "]
["isotig56662",-3.28003475836,30.987,3.19,"AT4G16280.2","FCA","RNA binding"]
["isotig53273",-3.07945633164494,17.43,2.062,"AT4G11050.1","AtGH9C3","Endoglucanase 19 "]
["isotig104695",-2.83171052196252,11.768,1.653,"AT2G26840.1",null,"unknown protein"]
["PUT-27230",-2.63912188654058,13.082,2.1,"AT1G24510.1","CCT5","T-complex protein 1 subunit epsilon "]
["isotig100954",-2.63881247466541,17.632,2.831,"AT5G05340.1","PER52","Peroxidase 52 ","response to stress"]
["isotig36281",-2.53198258906626,10.052,1.738,"AT2G13820.1",null,"Non-specific lipid-transfer protein-like protein At2g13820 "]
["isotig66171",-2.53171628317845,14.948,2.585,"AT1G11760.1","MED32","Mediator of RNA polymerase II transcription subunit 32 "]
["isotig77732",-2.53168569335574,10.819,1.871,"AT5G19380.1","CLT1","CRT (chloroquine-resistance transporter)-like transporter 1 "]
["PUT-21101",-2.41666791455996,19.478,3.648,"AT4G27780.1","ACBP2","Acyl-CoA-binding domain-containing protein 2 "]
["isotig89014",-2.29100635434873,10.801,2.207,"AT2G17695.1",null,"UPF0548 protein At2g17695 "]
["isotig55181",-2.15356209318885,10.082,2.266,"AT3G24490.1",null,"Alcohol dehydrogenase transcription factor Myb/SANT-like family protein "]
["isotig49131",-2.15323661361379,15.471,3.478,"AT1G36980.1",null,"unknown protein"]
["isotig84126",-2.15317706620158,13.66,3.071,"AT2G07671.1",null,"ATP synthase subunit C family protein "]
["isotig70115",-2.13524693188351,55.45,12.622,"AT3G22850.1",null,"Aluminium induced protein with YGL and LRDR motifs "]
["isotig50983",-2.07937284312622,13.693,3.24,"AT4G26500.1","SUFE1","SufE-like protein 1, chloroplastic/mitochondrial ","developmental process"]
["isotig88992",-2.05387836870184,13.64,3.285,"AT4G36040.1","ATJ11","Chaperone protein dnaJ 11, chloroplastic "]
["isotig83422",-2.05360330018125,12.612,3.038,"AT1G15010.1",null,"unknown protein"]
["isotig35197",-2.0014745719423,16.641,4.156,"AT3G12210.2",null,"DNA binding "]
["isotig99044",-2.00120352747101,10.793,2.696,"AT5G64260.1","EXL2","Protein EXORDIUM-like 2 "]

{"sheet":"M3xM4 Down","index":5,"n_rows":32,"n_cols":8,"rows_kept":32}
["Additional file 6: Differentially expressed transcripts (DETs) between consecutive megagametophyte developmental stages. Only DETs with positive matches against TAIR are shown"]
["Down-regulated transcripts in M3/M4 (34% were annotated). Annotated transcripts related to developmental process were represented in bold and green color and those related to response to stress and stimulus in bold and black. "]
[]
["Transcript ID","FC","M3","M4","At locus","At name","At annotation","GO term Biological process"]
["isotig114285",99,0,25.43,"AT1G09330.1","ECH","Golgi apparatus membrane protein-like protein ECHIDNA "]
["isotig95693",99,0,11.738,"AT3G60520.1",null,"unknown protein"]
["isotig106756",99,0,11.162,"AT5G48810.1","CYTB5-D","Cytochrome B5 isoform D "]
["PUT-43208",99,0,10.852,"AT1G16590.1","REV7","DNA polymerase zeta processivity subunit ","response to stimulus"]
["isotig102001",99,0,10.287,"AT1G77120.1","ADH1","Alcohol dehydrogenase class-P ","response to stress"]
["isotig53993",3.48985199771727,0.994,11.167,"AT5G21326.1","CIPK26","CBL-interacting serine/threonine-protein kinase 26 "]
["isotig103800",3.33856906710888,1.405,14.213,"AT2G38905.1",null,"Low temperature and salt responsive protein family ","response to stress"]
["isotig83269",2.9755486688754,1.8,14.158,"AT4G09610.1","GASA2","Gibberellin-regulated protein 2 ","response to hormone"]
["isotig85219",2.67087559167254,2.776,17.678,"AT5G66400.1","RAB18","Dehydrin Rab18 ","response to stress"]
["isotig46481",2.62783666028224,2.072,12.807,"AT5G27490.1",null,"Integral membrane Yip1 family protein "]
["isotig86820",2.49070037774345,1.892,10.634,"AT3G23390.1","RPL36AB","60S ribosomal protein L36a "]
["isotig107658",2.49035022643202,3.479,19.549,"AT4G36800.1","RCE1","NEDD8-conjugating enzyme Ubc12 ","embryonic development; response to hormone"]
["PUT-71351",2.49024245255862,3.087,17.345,"AT5G10770.1",null,"Eukaryotic aspartyl protease family protein "]
["isotig33229",2.40544418854358,2.235,11.841,"AT2G37630.1","AS1","Transcription factor AS1 ","response to hormone"]
["isotig23109",2.30591401053416,2.917,14.424,"AT1G66080.1",null,"unknown protein"]
["isotig58245",2.28419125107291,2.781,13.546,"AT2G26640.1","KCS11","3-ketoacyl-CoA synthase 11 ","response to stress"]
["isotig71133",2.28385793164603,2.181,10.621,"AT3G54420.1","ATEP3","homolog of carrot EP3-3 chitinase ","somatic embryogenesis; response to stress"]
["isotig67353",2.25571930306856,2.488,11.882,"AT2G23970.1",null,"Class I glutamine amidotransferase-like superfamily protein ","response to stimulus"]
["isotig34592",2.18962249357306,6.749,30.788,"AT1G17290.1","ALAAT1","Alanine aminotransferase 1, mitochondrial ","response to stress"]
["isotig66616",2.16878003204466,2.381,10.706,"AT5G19370.1",null,"Rhodanese-like/PpiC domain-containing protein 12 "]
["isotig90171",2.1684704272567,2.977,13.383,"AT1G79550.1","PGK","phosphoglycerate kinase "]
["isotig115105",2.16846337152684,3.621,16.278,"AT2G14620.1","XTH10","Probable xyloglucan endotransglucosylase/hydrolase protein 10 "]
["isotig63168",2.09469226834064,2.377,10.153,"AT3G60340.1",null,"alpha/beta-Hydrolases superfamily protein "]
["isotig37234",2.07516989945445,2.482,10.459,"AT5G34940.2","AtGUS3","Heparanase-like protein 3 "]
["isotig25220",2.04307946962467,2.961,12.203,"AT1G30890.1",null,"Integral membrane HRF1 family protein "]
["isotig80888",2.04307277850254,2.615,10.777,"ATMG00580.1","NAD4","NADH dehydrogenase subunit 4 "]
["isotig86476",2.04299014159061,2.835,11.683,"AT4G18240.1","SS4","Probable starch synthase 4, chloroplastic/amyloplastic "]
["isotig81766",2.04292455090452,2.649,10.916,"AT3G16770.1","RAP2-3","Ethylene-responsive transcription factor RAP2-3 ","response to stimulus"]