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["Additional Table 2 - Primers for VHL Sequencing"]
["VHL Exon","Primer Sequence","PCR Product Size","Melting Temperature"]
["Exon 1*","F: 5'-CGCGAAGACTACGGAGGT-3'","501 bp","58°C"]
[null,"R: 5'-GCTTCAGACCGTGCTATCGT-3'"]
["Exon 2","F: 5'-AACCTTTGCTTGTCCCGATA-3'","238 bp","54°C"]
[null,"R: 5'-CAGGCAAAAATTGAGAACTGG-3'"]
["Exon 3","F: 5'-CAGTGTCGCTTCATCCACAT-3'","699 bp","60°C"]
[null,"R: 5'-CAAAAATGCCACCACCTTCT-3'"]
["F: forward; R: reverse"]
["*1M betaine was added to the PCR reaction"]

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