LOCUS       Exported File           7214 bp ds-DNA    circular SYN 20-THG8-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    Pt TF FcpB-TwOSC4-Ble
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 7214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 20 Thg8 2025 from SnapGene 1.1.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7214
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /note="f1 ori"
                     /note="color: #ffff00"
     primer_bind     600..616
                     /note="M13 fwd"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        623..641
                     /note="T7 promoter"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        656..981
                     /note="FcpB promoter"
                     /note="color: #339966"
     5'UTR           990..1037
                     /note="FcpC 5'UTR"
                     /note="color: #a6acb3"
     misc_feature    1039..3346
                     /note="TwOSC4"
                     /note="color: #00ff00"
     terminator      3351..3634
                     /note="FcpA terminator"
                     /note="color: #ff6600"
     misc_feature    3671..4321
                     /note="FcpF promoter"
                     /note="color: #99cc00"
     CDS             4328..4702
                     /codon_start=1
                     /note="BleoR"
                     /note="color: #ccffcc"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      4709..4992
                     /note="FcpA terminator"
                     /note="color: #ff6600"
     promoter        complement(5026..5044)
                     /note="T3 promoter"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(5065..5081)
                     /note="M13 rev"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    5089..5105
                     /note="lac operator"
                     /note="color: #31849b"
     promoter        complement(5113..5143)
                     /note="lac promoter"
                     /note="This reverse directional feature has 3 segments:
                        1: 5113 .. 5119 / #ffffff / -10
                        2: 5120 .. 5137 / #ffffff
                        3: 5138 .. 5143 / #ffffff / -35"
     protein_bind    5158..5179
                     /note="CAP binding site"
                     /note="color: #31849b"
     rep_origin      complement(5467..6055)
                     /direction=LEFT
                     /note="ori"
                     /note="color: #ffff00"
     CDS             complement(6226..7086)
                     /codon_start=1
                     /note="AmpR"
                     /note="This feature has 2 segments:
                        1: 6226 .. 7017 / #ccffcc
                        2: 7018 .. 7086 / #ccffcc / signal sequence"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7087..7191)
                     /note="AmpR promoter"
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg agctcagtga
      661 ggaaggcaca ggatgagttt cctcgagaca catagcttca gcgtcgtgta ggctaggcag
      721 aggtgagttt tctcgagaca taccttcagc gtcgtcttca ctgtcacagt caactgacag
      781 taatcgttga tccggagaga ttcaaaattc aatctgtttg gacctggata agacacaaga
      841 gcgacatcct gacatgaacg ccgtaaacag caaatcctgg ttgaacacgt atccttttgg
      901 gggcctccag ctacgacgct cgccccagct ggggcttcct tactatacac agcgcatatt
      961 tcgcggttgc cagaagtcaa ggcggccgct aatctctgtt aaagtttacc aacaacacat
     1021 atatacattt cgacaaatct agaatgtgga agataaagat agcggaacgt gggaactgtc
     1081 cctactccga gtacttgtac acgacgaacg acttcgttgg tcgtcagatc tgggagtttg
     1141 acccgaacgc aggcaccccc gaagaactgg cggaggtcga agaggctcga aagaaattca
     1201 ccgagaaccg ccacgaagtc aagcctgctt ccgaccttct ttggatgcag cagtttctga
     1261 ggcagaagaa cttcaagcag accattcctc cgttgagaat tggcgagaaa gaacaagtta
     1321 cctatgaaga cgtcacgacc gctttgaaga tagcttcctc ttttttttca gccctgcagg
     1381 cctcggacgg tcactggcca gcagaaaatg ctggagtctt gtttttcctt cctccgttca
     1441 ttttctgcct atacatcacg ggacatctca attcgattat cacctcggaa caccgcaagg
     1501 aaattctgcg cttcatatac aaccaccaga atgaggatgg gggctgggga atacacatag
     1561 agggacacag ctcaatgttt accacaacat tcagctacgt ctgcatgaga atcctgggag
     1621 taggaccaga tgaaagtgct tgtgctagag cacgcaagtg gattcttgac cacggtggta
     1681 ttacttacat ggcttcctgg ggaaagacct ggctttcgat acttggtatc tttgattggt
     1741 atggatgcaa cccaatgccc ccagagttct ggattcttcc ctcttacctc cctatacacc
     1801 cagcgaagat gtggtgttat tgccggatgg tctacatgcc catgtcttac ctctatggga
     1861 aacggtttgt tggccctata acacctctca ttctgcaatt gagagaggag ctccacactc
     1921 agccctacca tgagattcaa tggcgtcaga tgcgccatcg atgcggaaag gaagatctat
     1981 actatcccca ctcgctcatc cagaacttcc tctgggactc cctttatgtg gcttccgaac
     2041 ctcttctgac tcgctggcct ttcagcaaga taagagagaa ggctcttgaa aaggcaatgg
     2101 aacacattca ctacgaagac gaaaacagtc gttacatcac cattggctgt gtggaaaagg
     2161 cattgtgcat gctttgttgc tgggtggaag accccaacgg tgactatttc aagaagcatc
     2221 ttgcaaggat acccgattat ctctgggttg ccgaggatgg tatgaaagtc gtgagtttcg
     2281 gctctcaatt gtgggatgct acctttggct ttcaagctct ggttgctagc aacctaacaa
     2341 acgaagtcgc tcccaccctc gtcaaagcat acgacttcgt caagaaatgc caggtccgga
     2401 ataacccctc tggcgacttt gagaaaatgt tccgccacat ttcaaaagga tcgtggacct
     2461 tctccgacca agatcatggc tggcaacttt ccgactgcac ggctgaggca ctgaagtgtt
     2521 gcttgctagc agcaaccatg cccgaagaac ttgtcggtga gaaattagat ccccaatgga
     2581 tatttgagtc tgtcaacatc atactatccc tccaagagcc gaaaaccggc ggtcttgctg
     2641 gctgggaacc agtacgcgct ggacaatgga tggagatgct caaccccatg gagtttctcg
     2701 agaacattgt gattgaacat acatatgtcg agtgcaccgg atcctcaatc attgctttca
     2761 ttactctcaa gaagctcttc ccaggtcacc gaaccaaaga catcgacaat ttcattgtca
     2821 acggtataag ataccttgaa gacgtccagt atcctgatgg ctcctggtac ggaaactggg
     2881 gcatttgctt catctatagt accatgtttg cactgggagg cctagcagca gctggtagga
     2941 cgtacgaaaa ttgtcccgcg gtgcgaagag gcgttgactt tttgcttatt aatcagagtg
     3001 atgatggtgg gtggggtgaa agctatcttt cttccccaag aaagatttat acaccccttg
     3061 aaggaagaag atcaaatgtg gtgcagactg catgggctat gctggggctt ctctatgctg
     3121 gccaggttga gcgcgaccct actcccctcc accgctgtgc gaaggtactg atcaattacc
     3181 aaatggaaga gggaggctac ccccagcagg aaatcactgg agttttcaaa atgaattgca
     3241 tgctccacta tccaatatat cgaaacgcct tcccgatctg ggcgctcgga gagtaccgga
     3301 accgtgtcaa gttgccttcg aaggaaatta agttgcaata aactagttaa cgtgctatcg
     3361 aactcaacca gggacgtgcg gcacaaatgg gcatccttgc tctcatggtg cacgaacagt
     3421 tgggagtctc tatccttcct taaaaattta attttcatta gttgcagtca ctccgctttg
     3481 gtttcacagt caggaataac actagctcgt cttcaccatg gatgccaatc tcgcccattc
     3541 atggtgtata caagttcaac atccaaagct ggaacttttg gaaacagaaa gaatgtccga
     3601 atagggcacg gcgtgccgta ttgttggagt ggacggatcc cccgggctgc aggaattcga
     3661 tatcaagctt ctaacaggat tagtgcaatt cgagttgaat cactgggaaa aacattgtct
     3721 tcttttttat attatcattt gcattagtgc tgcagtcgta gatacttgtt ggttgaaaga
     3781 catcagctgg gagggactgg actagcgttt ggtaaggaga catacctgtt aacgttggtt
     3841 gcaaaattcc atttcgcgat ttatgttatc tgtaaatcct gatttgtctg gaattcttga
     3901 tacttccgtt tttttagagg ccaatgatta gcatcggcga ttctcaaaat agcattttcg
     3961 acatgcggtg ctgatttcat aaacatagac aacgctttta catgtaaaag taacttgcgg
     4021 acttggaaca gtgctctgtt tttggtgtga acgtaactca gcaatatttc tgtgctagca
     4081 aggtttttta tgatcgaccg aagatctcaa aactccgggt ctttcaactg tctgactaga
     4141 ccatgttcgt aacgtcggac agcagctttc gttgtactgg tagaatttct acgtgcgaag
     4201 cacgtgtagg caggttgaac gacgatccct gccgatggat ggattggcac gcggcggaac
     4261 gctttcgtga tccacaccac ctggatcttc acatatcttc gaaatcgaaa aattaaccaa
     4321 gatcgatatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg
     4381 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
     4441 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
     4501 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg
     4561 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
     4621 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
     4681 cgtggccgag gagcaggact aagtcgaccg tgctatcgaa ctcaaccagg gacgtgcggc
     4741 acaaatgggc atccttgctc tcatggtgca cgaacagttg ggagtctcta tccttcctta
     4801 aaaatttaat tttcattagt tgcagtcact ccgctttggt ttcacagtca ggaataacac
     4861 tagctcgtct tcaccatgga tgccaatctc gcccattcat ggtgtataca agttcaacat
     4921 ccaaagctgg aacttttgga aacagaaaga atgtccgaat agggcacggc gtgccgtatt
     4981 gttggagtgg acctcgaggg ggggcccggt acccagcttt tgttcccttt agtgagggtt
     5041 aatttcgagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     5101 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     5161 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     5221 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     5281 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     5341 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     5401 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     5461 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     5521 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     5581 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     5641 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     5701 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     5761 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     5821 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     5881 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     5941 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     6001 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     6061 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     6121 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     6181 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     6241 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     6301 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     6361 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     6421 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     6481 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     6541 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     6601 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     6661 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     6721 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     6781 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     6841 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     6901 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     6961 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     7021 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     7081 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     7141 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     7201 ccgaaaagtg ccac
//
