urn:local:.:5j-7lneywh0A new nucleotide sequence entered manuallyP16_T7_nanolucP16:T7_nanoluc_3'HRPII_Puc1181615829476022145210545014309174DNAATGGTCTTCACACTCGAAGATTTCGTTGGGGACTGGCGACAGACAGCCGGCTACAACCTGGACCAAGTCCTTGAACAGGGAGGTGTGTCCAGTTTGTTTCAGAATCTCGGGGTGTCCGTAACTCCGATCCAAAGGATTGTCCTGAGCGGTGAAAATGGGCTGAAGATCGACATCCATGTCATCATCCCGTATGAAGGTCTGAGCGGCGACCAAATGGGCCAGATCGAAAAAATTTTTAAGGTGGTGTACCCTGTGGATGATCATCACTTTAAGGTGATCCTGCACTATGGCACACTGGTAATCGACGGGGTTACGCCGAACATGATCGACTATTTCGGACGGCCGTATGAAGGCATCGCCGTGTTCGACGGCAAAAAGATCACTGTAACAGGGACCCTGTGGAACGGCAACAAAATTATCGACGAGCGCCTGATCAACCCCGACGGCTCCCTGCTGTTCCGAGTAACCATCAACGGAGTGACCGGCTGGCGGCTGTGCGA4256circular1452105450143DescriptionA description of the sequencedescriptiontruejava.lang.Stringtrue1452105450143P16:T7_nanoluc_3'HRPII_Puc118A new nucleotide sequence entered manually3'HRP_UTRmisc_feature5171090leftToRightcreated byUserNanolucmisc_feature1516leftToRightcreated byUserColor#ff9911modified byUserT7_promotermisc_feature42384256leftToRightcreated byUserPVUIImisc_feature40544059leftToRightcreated byUserPVUIImisc_feature12141219leftToRightcreated byUserAPALImisc_feature18191824leftToRightcreated byUserAPALImisc_feature23162321leftToRightcreated byUserAPALImisc_feature35623567leftToRightcreated byUserATATAAAAAAATATATGCATTTAATAATAAATAGGAGAAAAAGTTGGATACGAAAAATAATAGGGGAAAAAAAAAATATTGTTATATATATATAATATATATATATAATATTAATTTATGCATATATTATTTATTATATATATTCCGATATTTATTACCAATTGTAAGCCCAAAATATTAATGTTAAAAAATACTTATTTTAAATATTATTAATTTATTATATTTTGTACGTAAAATTATTATTGTACATGTAAATAATTTGGATGAATGAATTGTTAATTATATAATATATTATTTATATTTATAAATAAATAATATATATTATATTAAAAGTAAATTTATACATACATATTTATGGTAATAGTAATTATAATTAATGTGGTATTTTCTTAAATATATATATATTATTATATATATATATATATATATTTATTTATTTTAATTATATATTTATGTATAAATATATTTTTTTCATATTTTTATTATTTTAAGAATTAAGTTATATTTTATTATATAATTTTTAAAAAAAATATTTTTAATACTTTTTTTTAAAATATAGAATGATATCTATATTCCATTTATAAAAATTAGATATTCATTAATATTATATATATTATATATATTTTATATATATTTATATATATTGAGAATTATTGTATTCCTTTTATATATTAATTATAATTTTTATTTATATTTTTACAAGTTCCATTATATATATATATATATATAAAATATATACTTTTATAAAATTTTTAAATATTAAAAAAAAAAAAAAEditing History Deletion10noneOriginal BasesATATAAAAAAATATATGCATTTAATAATAAATAGGAGAAAAAGTTGGATACGAAAAATAATAGGGGAAAAAAAAAATATTGTTATATATATATAATATATATATATAATATTAATTTATGCATATATTATTTATTATATATATTCCGATATTTATTACCAATTGTAAGCCCAAAATATTAATGTTAAAAAATACTTATTTTAAATATTATTAATTTATTATATTTTGTACGTAAAATTATTATTGTACATGTAAATAATTTGGATGAATGAATTGTTAATTATATAATATATTATTTATATTTATAAATAAATAATATATATTATATTAAAAGTAAATTTATACATACATATTTATGGTAATAGTAATTATAATTAATGTGGTATTTTCTTAAATATATATATATTATTATATATATATATATATATATTTATTTATTTTAATTATATATTTATGTATAAATATATTTTTTTCATATTTTTATTATTTTAAGAATTAAGTTATATTTTATTATATAATTTTTAAAAAAAATATTTTTAATACTTTTTTTTAAAATATAGAATGATATCTATATTCCATTTATAAAAATTAGATATTCATTAATATTATATATATTATATATATTTTATATATATTTATATATATTGAGAATTATTGTATTCCTTTTATATATTAATTATAATTTTTATTTATATTTTTACAAGTTCCATTATATATATATATATATATAAAATATATACTTTTATAAAATTTTTAAATATTAAAAAAAAAAAAAAATGGTCTTCACACTCGAAGATTTCGTTGGGGACTGGCGACAGACAGCCGGCTACAACCTGGACCAAGTCCTTGAACAGGGAGGTGTGTCCAGTTTGTTTCAGAATCTCGGGGTGTCCGTAACTCCGATCCAAAGGATTGTCCTGAGCGGTGAAAATGGGCTGAAGATCGACATCCATGTCATCATCCCGTATGAAGGTCTGAGCGGCGACCAAATGGGCCAGATCGAAAAAATTTTTAAGGTGGTGTACCCTGTGGATGATCATCACTTTAAGGTGATCCTGCACTATGGCACACTGGTAATCGACGGGGTTACGCCGAACATGATCGACTATTTCGGACGGCCGTATGAAGGCATCGCCGTGTTCGACGGCAAAAAGATCACTGTAACAGGGACCCTGTGGAACGGCAACAAAATTATCGACGAGCGCCTGATCAACCCCGACGGCTCCCTGCTGTTCCGAGTAACCATCAACGGAGTGACCGGCTGGCGGCTGTGCGAACGCATTCTGGCGTAATTCTAGAAGCTTATTTAATAATAGATTAAAAATATTATAAAAATAAAAACATAAACACAGAAATTACAAAAAAAATACATATGAATTTTTTTTTGTAATCTTCCTTATAAATATAGAATAATGAATCATATAAAACATATCATTATTCATTTATTTACATTTAAAATTATTGTTTCAGTATCTTTAATTTATTATGTATATATAAAAATAACTTACAATTTTATTAATAAACAATATATGTTTATTAATTCATGTTTTGTAATTTATGGGATAGCGATTTTTTTTACTGTCTGTATTTTTCTTTTTTAATTATGTTTTAATTGTATTTTATTTTTATTATTGTTCTTTTTATAGTATTATTTTAAAACAAAATGTATTTTCTAAGAACTTATAATAATAATAAATATAAATTTTAATAAAAATTATATTTATCTTTTACAATATGAACATAAAGTACAACATTAATATATAGCTTTTAATATTTTTATTCCTAATCATGTAAATCTTAAATTTTTCTTTTTAAACATATGTTAAATATTTATTTCTCATTATATATAAGAACATATTTATTAAAGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGGCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATACGTCAAAGCAACCATAGTACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTTGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGGCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTTTATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGAATTCTAATACGACTCACTATAGG0.0102570505369277234170418701000-41Frame1sequencefalsefalsefalse(1, 190)[1,4256)0.0053973058800055050252211Frame1sequencebackground1RasMolHydrophobicityPolarityRasMolClustalMacCladeAnimated :-)By Annotation255,255,255;0,0,0;3,3,3255,255,255;175,161,1;250,230,4255,255,255;178,0,0;254,0,0255,255,255;255,0,255;255,0,255255,255,255;64,64,64;91,91,91255,255,255;107,142,35;152,202,50255,255,255;139,69,19;198,98,27255,255,255;46,139,87;65,198,124255,255,255;0,0,255;0,0,255255,255,255;176,48,96;251,68,137255,255,255;199,21,133;255,30,190255,255,255;0,178,178;0,254,254255,255,255;255,200,0;255,255,0255,255,255;70,130,180;100,185,255255,255,255;95,158,160;135,225,228255,255,255;250,128,114;255,182,162255,255,255;244,164,96;255,234,137255,255,255;0,178,0;0,254,0255,255,255;178,122,122;254,174,174255,255,255;0,100,0;0,142,0255,255,255;184,134,11;255,191,15255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;200,200,200;255,255,255255,255,255;230,230,0;255,255,0255,255,255;230,10,10;255,14,14255,255,255;230,10,10;255,14,14255,255,255;50,50,170;71,71,242255,255,255;235,235,235;255,255,255255,255,255;130,130,210;185,185,255255,255,255;15,130,15;21,185,21255,255,255;20,90,255;28,128,255255,255,255;15,130,15;21,185,21255,255,255;230,230,0;255,255,0255,255,255;0,220,220;0,255,255255,255,255;220,150,130;255,214,185255,255,255;0,220,220;0,255,255255,255,255;20,90,255;28,128,255255,255,255;250,150,0;255,214,0255,255,255;250,150,0;255,214,0255,255,255;15,130,15;21,185,21255,255,255;180,90,180;255,128,255255,255,255;50,50,170;71,71,242255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3192,192,192;0,0,0;192,192,192251,231,2;0,0,0;251,231,2255,120,120;0,0,0;255,120,120255,0,255;0,0,0;255,0,255240,230,140;0,0,0;240,230,140152,202,50;0,0,0;152,202,50255,140,38;0,0,0;255,140,3865,198,124;0,0,0;65,198,124135,206,250;0,0,0;135,206,250251,68,137;0,0,0;251,68,137255,42,255;0,0,0;255,42,2550,255,255;0,0,0;0,255,255255,255,0;0,0,0;255,255,0100,185,255;0,0,0;100,185,255135,225,228;0,0,0;135,225,228255,182,162;0,0,0;255,182,162255,234,137;0,0,0;255,234,1370,255,0;0,0,0;0,255,0255,175,175;0,0,0;255,175,175173,255,47;0,0,0;173,255,47255,191,15;0,0,0;255,191,15100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,1000,0,0;170,170,170;0,0,0255,255,255;0,0,0;3,3,3157,0,98;255,255,255;157,0,98173,0,82;255,255,255;173,0,827,0,248;255,255,255;7,0,24810,0,245;255,255,255;10,0,245255,0,0;255,255,255;255,0,0127,0,128;255,255,255;127,0,12842,0,213;255,255,255;42,0,213240,0,15;255,255,255;240,0,1572,0,183;255,255,255;72,0,183240,0,15;255,255,255;240,0,15188,0,67;255,255,255;188,0,6760,0,195;255,255,255;60,0,195181,0,74;255,255,255;181,0,7464,0,191;255,255,255;64,0,1910,0,255;255,255,255;0,0,25591,0,164;255,255,255;91,0,164114,0,141;255,255,255;114,0,141210,0,45;255,255,255;210,0,45223,0,32;255,255,255;223,0,32224,0,31;255,255,255;224,0,31100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,1000,0,0;170,170,170;0,0,0255,255,255;0,0,0;3,3,3175,161,1;255,255,255;175,161,10,178,0;255,255,255;0,178,0178,0,0;255,255,255;178,0,0178,0,0;255,255,255;178,0,0175,161,1;255,255,255;175,161,1175,161,1;255,255,255;175,161,10,0,178;255,255,255;0,0,178175,161,1;255,255,255;175,161,10,0,178;255,255,255;0,0,178175,161,1;255,255,255;175,161,1175,161,1;255,255,255;175,161,10,178,0;255,255,255;0,178,0175,161,1;255,255,255;175,161,10,178,0;255,255,255;0,178,00,0,178;255,255,255;0,0,1780,178,0;255,255,255;0,178,00,178,0;255,255,255;0,178,0175,161,1;255,255,255;175,161,1175,161,1;255,255,255;175,161,10,178,0;255,255,255;0,178,00,178,0;255,255,255;0,178,0100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,1000,0,0;170,170,170;0,0,0255,255,255;0,0,0;3,3,3200,200,200;0,0,0;200,200,200230,230,0;0,0,0;230,230,0230,10,10;0,0,0;230,10,10230,10,10;0,0,0;230,10,1050,50,170;0,0,0;50,50,170235,235,235;0,0,0;235,235,235130,130,210;0,0,0;130,130,21015,130,15;0,0,0;15,130,1520,90,255;0,0,0;20,90,25515,130,15;0,0,0;15,130,15230,230,0;0,0,0;230,230,00,220,220;0,0,0;0,220,220220,150,130;0,0,0;220,150,1300,220,220;0,0,0;0,220,22020,90,255;0,0,0;20,90,255250,150,0;0,0,0;250,150,0250,150,0;0,0,0;250,150,015,130,15;0,0,0;15,130,15180,90,180;0,0,0;180,90,18050,50,170;0,0,0;50,50,170100,100,100;0,0,0;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100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Documents/PlasMapper Features[]10truetruetrue100universalTTG, CTG, ATGfalsefalsefalseall_enzymestrue4falsepalindromictrue(1, 2)anywhere(1, 2)[1,1000)-1commonly_used_enzymes0.005167958604651163125300-6Frame1sequencebackground1RasMolHydrophobicityPolarityRasMolClustalMacCladeAnimated :-)By 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Documents/PlasMapper Features[]10truetruetrue100universalATG, CTG, TTGfalsefalsefalseall_enzymestrue4falsepalindromictrue(1, 2)anywhere(1, 2)[1,5031)-1commonly_used_enzymes0.00516795860465116312530040Frame1sequencebackground1RasMolHydrophobicityPolarityRasMolClustalMacCladeAnimated :-)By 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Documents/PlasMapper Features[]10truetruetrue100universalCTG, ATG, TTGfalsefalsefalseall_enzymestrue4falsepalindromictrue(1, 2)anywhere(1, 2)[1,5031)-1all_enzymes0.00706389608091371912526030Frame1sequencebackground1RasMolHydrophobicityPolarityRasMolClustalMacCladeAnimated :-)By 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Documents/PlasMapper Features[]10truetruetrue100universalTTG, CTG, ATGfalsefalsefalseall_enzymestrue4falsepalindromictrue(1, 2)anywhere(1, 2)[1,1000)-1all_enzymes0.00541259569836245902529143Frame1sequencebackground1RasMolHydrophobicityPolarityRasMolClustalMacCladeAnimated :-)By 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Documents/PlasMapper Features[]10truetruetrue100universalTTG, CTG, ATGfalsefalsefalseall_enzymestrue4falsepalindromictrue(1, 2)anywhere(1, 2)[1,5031)-1commonly_used_enzymes0.00516917288063909812142136Frame1sequencebackground1RasMolHydrophobicityPolarityRasMolClustalMacCladeAnimated :-)By 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Documents/PlasMapper Features[]10truetruetrue100universalTTG, CTG, ATGfalsefalsefalseblunt_cutters_(commercially_available)true4falsepalindromictrue(1, 2)anywhere(1, 2)[1,688)-1commonly_used_enzymes0.009082152104031925125231-28Frame1sequencebackground1RasMolHydrophobicityPolarityRasMolClustalMacCladeAnimated :-)By 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Documents/PlasMapper Features[]10truetruetrue100universalATG, TTG, CTGfalsefalsefalseall_enzymestrue4falsepalindromictrue(1, 2)anywhere(1, 2)[1,5031)-1commonly_used_enzymes0.00541259569836245902529143Frame1sequencebackground1RasMolHydrophobicityPolarityRasMolClustalMacCladeAnimated :-)By 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Documents/PlasMapper Features[]10truetruetrue100universalTTG, CTG, ATGfalsefalsefalseall_enzymestrue4falsepalindromictrue(1, 2)anywhere(1, 2)[1,5031)-1commonly_used_enzymes0.49999999999999994000100-3-296Frame1sequencebackground1RasMolHydrophobicityPolarityRasMolClustalMacCladeAnimated :-)By 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Documents/PlasMapper Features[]10truetruetrue100universalCTG, ATG, TTGfalsefalsetrueall_enzymestrue4falsepalindromictrue(1, 2)anywhere(1, 2)[1,4256)-1commonly_used_enzymes0.0086234575533232412524045Frame1sequencebackground1RasMolHydrophobicityPolarityRasMolClustalMacCladeAnimated :-)By Annotation255,255,255;0,0,0;3,3,3255,255,255;175,161,1;250,230,4255,255,255;178,0,0;254,0,0255,255,255;255,0,255;255,0,255255,255,255;64,64,64;91,91,91255,255,255;107,142,35;152,202,50255,255,255;139,69,19;198,98,27255,255,255;46,139,87;65,198,124255,255,255;0,0,255;0,0,255255,255,255;176,48,96;251,68,137255,255,255;199,21,133;255,30,190255,255,255;0,178,178;0,254,254255,255,255;255,200,0;255,255,0255,255,255;70,130,180;100,185,255255,255,255;95,158,160;135,225,228255,255,255;250,128,114;255,182,162255,255,255;244,164,96;255,234,137255,255,255;0,178,0;0,254,0255,255,255;178,122,122;254,174,174255,255,255;0,100,0;0,142,0255,255,255;184,134,11;255,191,15255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;200,200,200;255,255,255255,255,255;230,230,0;255,255,0255,255,255;230,10,10;255,14,14255,255,255;230,10,10;255,14,14255,255,255;50,50,170;71,71,242255,255,255;235,235,235;255,255,255255,255,255;130,130,210;185,185,255255,255,255;15,130,15;21,185,21255,255,255;20,90,255;28,128,255255,255,255;15,130,15;21,185,21255,255,255;230,230,0;255,255,0255,255,255;0,220,220;0,255,255255,255,255;220,150,130;255,214,185255,255,255;0,220,220;0,255,255255,255,255;20,90,255;28,128,255255,255,255;250,150,0;255,214,0255,255,255;250,150,0;255,214,0255,255,255;15,130,15;21,185,21255,255,255;180,90,180;255,128,255255,255,255;50,50,170;71,71,242255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3192,192,192;0,0,0;192,192,192251,231,2;0,0,0;251,231,2255,120,120;0,0,0;255,120,120255,0,255;0,0,0;255,0,255240,230,140;0,0,0;240,230,140152,202,50;0,0,0;152,202,50255,140,38;0,0,0;255,140,3865,198,124;0,0,0;65,198,124135,206,250;0,0,0;135,206,250251,68,137;0,0,0;251,68,137255,42,255;0,0,0;255,42,2550,255,255;0,0,0;0,255,255255,255,0;0,0,0;255,255,0100,185,255;0,0,0;100,185,255135,225,228;0,0,0;135,225,228255,182,162;0,0,0;255,182,162255,234,137;0,0,0;255,234,1370,255,0;0,0,0;0,255,0255,175,175;0,0,0;255,175,175173,255,47;0,0,0;173,255,47255,191,15;0,0,0;255,191,15100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,1000,0,0;170,170,170;0,0,0255,255,255;0,0,0;3,3,3157,0,98;255,255,255;157,0,98173,0,82;255,255,255;173,0,827,0,248;255,255,255;7,0,24810,0,245;255,255,255;10,0,245255,0,0;255,255,255;255,0,0127,0,128;255,255,255;127,0,12842,0,213;255,255,255;42,0,213240,0,15;255,255,255;240,0,1572,0,183;255,255,255;72,0,183240,0,15;255,255,255;240,0,15188,0,67;255,255,255;188,0,6760,0,195;255,255,255;60,0,195181,0,74;255,255,255;181,0,7464,0,191;255,255,255;64,0,1910,0,255;255,255,255;0,0,25591,0,164;255,255,255;91,0,164114,0,141;255,255,255;114,0,141210,0,45;255,255,255;210,0,45223,0,32;255,255,255;223,0,32224,0,31;255,255,255;224,0,31100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,1000,0,0;170,170,170;0,0,0255,255,255;0,0,0;3,3,3175,161,1;255,255,255;175,161,10,178,0;255,255,255;0,178,0178,0,0;255,255,255;178,0,0178,0,0;255,255,255;178,0,0175,161,1;255,255,255;175,161,1175,161,1;255,255,255;175,161,10,0,178;255,255,255;0,0,178175,161,1;255,255,255;175,161,10,0,178;255,255,255;0,0,178175,161,1;255,255,255;175,161,1175,161,1;255,255,255;175,161,10,178,0;255,255,255;0,178,0175,161,1;255,255,255;175,161,10,178,0;255,255,255;0,178,00,0,178;255,255,255;0,0,1780,178,0;255,255,255;0,178,00,178,0;255,255,255;0,178,0175,161,1;255,255,255;175,161,1175,161,1;255,255,255;175,161,10,178,0;255,255,255;0,178,00,178,0;255,255,255;0,178,0100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,1000,0,0;170,170,170;0,0,0255,255,255;0,0,0;3,3,3200,200,200;0,0,0;200,200,200230,230,0;0,0,0;230,230,0230,10,10;0,0,0;230,10,10230,10,10;0,0,0;230,10,1050,50,170;0,0,0;50,50,170235,235,235;0,0,0;235,235,235130,130,210;0,0,0;130,130,21015,130,15;0,0,0;15,130,1520,90,255;0,0,0;20,90,25515,130,15;0,0,0;15,130,15230,230,0;0,0,0;230,230,00,220,220;0,0,0;0,220,220220,150,130;0,0,0;220,150,1300,220,220;0,0,0;0,220,22020,90,255;0,0,0;20,90,255250,150,0;0,0,0;250,150,0250,150,0;0,0,0;250,150,015,130,15;0,0,0;15,130,15180,90,180;0,0,0;180,90,18050,50,170;0,0,0;50,50,170100,100,100;0,0,0;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100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Documents/PlasMapper Features[]10truetruetrue100universalCTG, ATG, TTGfalsefalsefalseall_enzymestrue4falsepalindromictrue(1, 2)anywhere(1, 2)[1,5031)-1commonly_used_enzymes urn:local:.:5j-7lneywh0A new nucleotide sequence entered manuallyP16_T7_nanolucP16:T7_nanoluc_3'HRPII_Puc1181615829476022145210545014309174DNAATGGTCTTCACACTCGAAGATTTCGTTGGGGACTGGCGACAGACAGCCGGCTACAACCTGGACCAAGTCCTTGAACAGGGAGGTGTGTCCAGTTTGTTTCAGAATCTCGGGGTGTCCGTAACTCCGATCCAAAGGATTGTCCTGAGCGGTGAAAATGGGCTGAAGATCGACATCCATGTCATCATCCCGTATGAAGGTCTGAGCGGCGACCAAATGGGCCAGATCGAAAAAATTTTTAAGGTGGTGTACCCTGTGGATGATCATCACTTTAAGGTGATCCTGCACTATGGCACACTGGTAATCGACGGGGTTACGCCGAACATGATCGACTATTTCGGACGGCCGTATGAAGGCATCGCCGTGTTCGACGGCAAAAAGATCACTGTAACAGGGACCCTGTGGAACGGCAACAAAATTATCGACGAGCGCCTGATCAACCCCGACGGCTCCCTGCTGTTCCGAGTAACCATCAACGGAGTGACCGGCTGGCGGCTGTGCGA4256circular1452105450143DescriptionA description of the sequencedescriptiontruejava.lang.Stringtrue1452105450143P16_T7_nanolucA new nucleotide sequence entered manually3'HRP_UTRmisc_feature5171090leftToRightcreated byUserNanolucmisc_feature1516leftToRightcreated byUserColor#ff9911modified byUserT7_promotermisc_feature42384256leftToRightcreated byUserPVUIImisc_feature40544059leftToRightcreated byUserPVUIImisc_feature12141219leftToRightcreated byUserAPALImisc_feature18191824leftToRightcreated byUserAPALImisc_feature23162321leftToRightcreated byUserAPALImisc_feature35623567leftToRightcreated byUserATATAAAAAAATATATGCATTTAATAATAAATAGGAGAAAAAGTTGGATACGAAAAATAATAGGGGAAAAAAAAAATATTGTTATATATATATAATATATATATATAATATTAATTTATGCATATATTATTTATTATATATATTCCGATATTTATTACCAATTGTAAGCCCAAAATATTAATGTTAAAAAATACTTATTTTAAATATTATTAATTTATTATATTTTGTACGTAAAATTATTATTGTACATGTAAATAATTTGGATGAATGAATTGTTAATTATATAATATATTATTTATATTTATAAATAAATAATATATATTATATTAAAAGTAAATTTATACATACATATTTATGGTAATAGTAATTATAATTAATGTGGTATTTTCTTAAATATATATATATTATTATATATATATATATATATATTTATTTATTTTAATTATATATTTATGTATAAATATATTTTTTTCATATTTTTATTATTTTAAGAATTAAGTTATATTTTATTATATAATTTTTAAAAAAAATATTTTTAATACTTTTTTTTAAAATATAGAATGATATCTATATTCCATTTATAAAAATTAGATATTCATTAATATTATATATATTATATATATTTTATATATATTTATATATATTGAGAATTATTGTATTCCTTTTATATATTAATTATAATTTTTATTTATATTTTTACAAGTTCCATTATATATATATATATATATAAAATATATACTTTTATAAAATTTTTAAATATTAAAAAAAAAAAAAAEditing History Deletion10noneOriginal BasesATATAAAAAAATATATGCATTTAATAATAAATAGGAGAAAAAGTTGGATACGAAAAATAATAGGGGAAAAAAAAAATATTGTTATATATATATAATATATATATATAATATTAATTTATGCATATATTATTTATTATATATATTCCGATATTTATTACCAATTGTAAGCCCAAAATATTAATGTTAAAAAATACTTATTTTAAATATTATTAATTTATTATATTTTGTACGTAAAATTATTATTGTACATGTAAATAATTTGGATGAATGAATTGTTAATTATATAATATATTATTTATATTTATAAATAAATAATATATATTATATTAAAAGTAAATTTATACATACATATTTATGGTAATAGTAATTATAATTAATGTGGTATTTTCTTAAATATATATATATTATTATATATATATATATATATATTTATTTATTTTAATTATATATTTATGTATAAATATATTTTTTTCATATTTTTATTATTTTAAGAATTAAGTTATATTTTATTATATAATTTTTAAAAAAAATATTTTTAATACTTTTTTTTAAAATATAGAATGATATCTATATTCCATTTATAAAAATTAGATATTCATTAATATTATATATATTATATATATTTTATATATATTTATATATATTGAGAATTATTGTATTCCTTTTATATATTAATTATAATTTTTATTTATATTTTTACAAGTTCCATTATATATATATATATATATAAAATATATACTTTTATAAAATTTTTAAATATTAAAAAAAAAAAAAAATGGTCTTCACACTCGAAGATTTCGTTGGGGACTGGCGACAGACAGCCGGCTACAACCTGGACCAAGTCCTTGAACAGGGAGGTGTGTCCAGTTTGTTTCAGAATCTCGGGGTGTCCGTAACTCCGATCCAAAGGATTGTCCTGAGCGGTGAAAATGGGCTGAAGATCGACATCCATGTCATCATCCCGTATGAAGGTCTGAGCGGCGACCAAATGGGCCAGATCGAAAAAATTTTTAAGGTGGTGTACCCTGTGGATGATCATCACTTTAAGGTGATCCTGCACTATGGCACACTGGTAATCGACGGGGTTACGCCGAACATGATCGACTATTTCGGACGGCCGTATGAAGGCATCGCCGTGTTCGACGGCAAAAAGATCACTGTAACAGGGACCCTGTGGAACGGCAACAAAATTATCGACGAGCGCCTGATCAACCCCGACGGCTCCCTGCTGTTCCGAGTAACCATCAACGGAGTGACCGGCTGGCGGCTGTGCGAACGCATTCTGGCGTAATTCTAGAAGCTTATTTAATAATAGATTAAAAATATTATAAAAATAAAAACATAAACACAGAAATTACAAAAAAAATACATATGAATTTTTTTTTGTAATCTTCCTTATAAATATAGAATAATGAATCATATAAAACATATCATTATTCATTTATTTACATTTAAAATTATTGTTTCAGTATCTTTAATTTATTATGTATATATAAAAATAACTTACAATTTTATTAATAAACAATATATGTTTATTAATTCATGTTTTGTAATTTATGGGATAGCGATTTTTTTTACTGTCTGTATTTTTCTTTTTTAATTATGTTTTAATTGTATTTTATTTTTATTATTGTTCTTTTTATAGTATTATTTTAAAACAAAATGTATTTTCTAAGAACTTATAATAATAATAAATATAAATTTTAATAAAAATTATATTTATCTTTTACAATATGAACATAAAGTACAACATTAATATATAGCTTTTAATATTTTTATTCCTAATCATGTAAATCTTAAATTTTTCTTTTTAAACATATGTTAAATATTTATTTCTCATTATATATAAGAACATATTTATTAAAGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGGCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATACGTCAAAGCAACCATAGTACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTTGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGGCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTTTATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGAATTCTAATACGACTCACTATAGG0.0102570505369277234170418701000-41Frame1sequencefalsefalsefalse(1, 190)[1,4256)0.0053973058800055050252211Frame1sequencebackground1RasMolHydrophobicityPolarityRasMolClustalMacCladeAnimated :-)By Annotation255,255,255;0,0,0;3,3,3255,255,255;175,161,1;250,230,4255,255,255;178,0,0;254,0,0255,255,255;255,0,255;255,0,255255,255,255;64,64,64;91,91,91255,255,255;107,142,35;152,202,50255,255,255;139,69,19;198,98,27255,255,255;46,139,87;65,198,124255,255,255;0,0,255;0,0,255255,255,255;176,48,96;251,68,137255,255,255;199,21,133;255,30,190255,255,255;0,178,178;0,254,254255,255,255;255,200,0;255,255,0255,255,255;70,130,180;100,185,255255,255,255;95,158,160;135,225,228255,255,255;250,128,114;255,182,162255,255,255;244,164,96;255,234,137255,255,255;0,178,0;0,254,0255,255,255;178,122,122;254,174,174255,255,255;0,100,0;0,142,0255,255,255;184,134,11;255,191,15255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;200,200,200;255,255,255255,255,255;230,230,0;255,255,0255,255,255;230,10,10;255,14,14255,255,255;230,10,10;255,14,14255,255,255;50,50,170;71,71,242255,255,255;235,235,235;255,255,255255,255,255;130,130,210;185,185,255255,255,255;15,130,15;21,185,21255,255,255;20,90,255;28,128,255255,255,255;15,130,15;21,185,21255,255,255;230,230,0;255,255,0255,255,255;0,220,220;0,255,255255,255,255;220,150,130;255,214,185255,255,255;0,220,220;0,255,255255,255,255;20,90,255;28,128,255255,255,255;250,150,0;255,214,0255,255,255;250,150,0;255,214,0255,255,255;15,130,15;21,185,21255,255,255;180,90,180;255,128,255255,255,255;50,50,170;71,71,242255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3255,255,255;0,0,0;3,3,3192,192,192;0,0,0;192,192,192251,231,2;0,0,0;251,231,2255,120,120;0,0,0;255,120,120255,0,255;0,0,0;255,0,255240,230,140;0,0,0;240,230,140152,202,50;0,0,0;152,202,50255,140,38;0,0,0;255,140,3865,198,124;0,0,0;65,198,124135,206,250;0,0,0;135,206,250251,68,137;0,0,0;251,68,137255,42,255;0,0,0;255,42,2550,255,255;0,0,0;0,255,255255,255,0;0,0,0;255,255,0100,185,255;0,0,0;100,185,255135,225,228;0,0,0;135,225,228255,182,162;0,0,0;255,182,162255,234,137;0,0,0;255,234,1370,255,0;0,0,0;0,255,0255,175,175;0,0,0;255,175,175173,255,47;0,0,0;173,255,47255,191,15;0,0,0;255,191,15100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,1000,0,0;170,170,170;0,0,0255,255,255;0,0,0;3,3,3157,0,98;255,255,255;157,0,98173,0,82;255,255,255;173,0,827,0,248;255,255,255;7,0,24810,0,245;255,255,255;10,0,245255,0,0;255,255,255;255,0,0127,0,128;255,255,255;127,0,12842,0,213;255,255,255;42,0,213240,0,15;255,255,255;240,0,1572,0,183;255,255,255;72,0,183240,0,15;255,255,255;240,0,15188,0,67;255,255,255;188,0,6760,0,195;255,255,255;60,0,195181,0,74;255,255,255;181,0,7464,0,191;255,255,255;64,0,1910,0,255;255,255,255;0,0,25591,0,164;255,255,255;91,0,164114,0,141;255,255,255;114,0,141210,0,45;255,255,255;210,0,45223,0,32;255,255,255;223,0,32224,0,31;255,255,255;224,0,31100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,1000,0,0;170,170,170;0,0,0255,255,255;0,0,0;3,3,3175,161,1;255,255,255;175,161,10,178,0;255,255,255;0,178,0178,0,0;255,255,255;178,0,0178,0,0;255,255,255;178,0,0175,161,1;255,255,255;175,161,1175,161,1;255,255,255;175,161,10,0,178;255,255,255;0,0,178175,161,1;255,255,255;175,161,10,0,178;255,255,255;0,0,178175,161,1;255,255,255;175,161,1175,161,1;255,255,255;175,161,10,178,0;255,255,255;0,178,0175,161,1;255,255,255;175,161,10,178,0;255,255,255;0,178,00,0,178;255,255,255;0,0,1780,178,0;255,255,255;0,178,00,178,0;255,255,255;0,178,0175,161,1;255,255,255;175,161,1175,161,1;255,255,255;175,161,10,178,0;255,255,255;0,178,00,178,0;255,255,255;0,178,0100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,1000,0,0;170,170,170;0,0,0255,255,255;0,0,0;3,3,3200,200,200;0,0,0;200,200,200230,230,0;0,0,0;230,230,0230,10,10;0,0,0;230,10,10230,10,10;0,0,0;230,10,1050,50,170;0,0,0;50,50,170235,235,235;0,0,0;235,235,235130,130,210;0,0,0;130,130,21015,130,15;0,0,0;15,130,1520,90,255;0,0,0;20,90,25515,130,15;0,0,0;15,130,15230,230,0;0,0,0;230,230,00,220,220;0,0,0;0,220,220220,150,130;0,0,0;220,150,1300,220,220;0,0,0;0,220,22020,90,255;0,0,0;20,90,255250,150,0;0,0,0;250,150,0250,150,0;0,0,0;250,150,015,130,15;0,0,0;15,130,15180,90,180;0,0,0;180,90,18050,50,170;0,0,0;50,50,170100,100,100;0,0,0;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100,100100,100,100;255,255,255;100,100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Documents/PlasMapper Features[]10truetruetrue100universalTTG, CTG, ATGfalsefalsefalseall_enzymestrue4falsepalindromictrue(1, 2)anywhere(1, 2)[1,1000)-1commonly_used_enzymes0.005167958604651163125300-6Frame1sequencebackground1RasMolHydrophobicityPolarityRasMolClustalMacCladeAnimated :-)By 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Documents/PlasMapper Features[]10truetruetrue100universalATG, CTG, TTGfalsefalsefalseall_enzymestrue4falsepalindromictrue(1, 2)anywhere(1, 2)[1,5031)-1commonly_used_enzymes0.00516795860465116312530040Frame1sequencebackground1RasMolHydrophobicityPolarityRasMolClustalMacCladeAnimated :-)By 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Documents/PlasMapper Features[]10truetruetrue100universalCTG, ATG, TTGfalsefalsefalseall_enzymestrue4falsepalindromictrue(1, 2)anywhere(1, 2)[1,5031)-1all_enzymes0.00706389608091371912526030Frame1sequencebackground1RasMolHydrophobicityPolarityRasMolClustalMacCladeAnimated :-)By 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Documents/PlasMapper Features[]10truetruetrue100universalTTG, CTG, ATGfalsefalsefalseall_enzymestrue4falsepalindromictrue(1, 2)anywhere(1, 2)[1,1000)-1all_enzymes0.00541259569836245902529143Frame1sequencebackground1RasMolHydrophobicityPolarityRasMolClustalMacCladeAnimated :-)By 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Documents/PlasMapper Features[]10truetruetrue100universalTTG, CTG, ATGfalsefalsefalseall_enzymestrue4falsepalindromictrue(1, 2)anywhere(1, 2)[1,5031)-1commonly_used_enzymes0.00516917288063909812142136Frame1sequencebackground1RasMolHydrophobicityPolarityRasMolClustalMacCladeAnimated :-)By 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Documents/PlasMapper Features[]10truetruetrue100universalTTG, CTG, ATGfalsefalsefalseblunt_cutters_(commercially_available)true4falsepalindromictrue(1, 2)anywhere(1, 2)[1,688)-1commonly_used_enzymes0.009082152104031925125231-28Frame1sequencebackground1RasMolHydrophobicityPolarityRasMolClustalMacCladeAnimated :-)By 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Documents/PlasMapper Features[]10truetruetrue100universalATG, TTG, CTGfalsefalsefalseall_enzymestrue4falsepalindromictrue(1, 2)anywhere(1, 2)[1,5031)-1commonly_used_enzymes0.00541259569836245902529143Frame1sequencebackground1RasMolHydrophobicityPolarityRasMolClustalMacCladeAnimated :-)By 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Documents/PlasMapper Features[]10truetruetrue100universalTTG, CTG, ATGfalsefalsefalseall_enzymestrue4falsepalindromictrue(1, 2)anywhere(1, 2)[1,5031)-1commonly_used_enzymes0.49999999999999994000100-3-296Frame1sequencebackground1RasMolHydrophobicityPolarityRasMolClustalMacCladeAnimated :-)By 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Documents/PlasMapper Features[]10truetruetrue100universalCTG, ATG, TTGfalsefalsetrueall_enzymestrue4falsepalindromictrue(1, 2)anywhere(1, 2)[1,4256)-1commonly_used_enzymes0.0086234575533232412524045Frame1sequencebackground1RasMolHydrophobicityPolarityRasMolClustalMacCladeAnimated :-)By 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Documents/PlasMapper Features[]10truetruetrue100universalCTG, ATG, TTGfalsefalsefalseall_enzymestrue4falsepalindromictrue(1, 2)anywhere(1, 2)[1,5031)-1commonly_used_enzymes 1615829516752This file was created using Geneious 6.0 or later.1615829516752urn:local:.:b-d4pjunv30