LOCUS Exported 11196 bp ds-DNA circular SYN 17-MAY-2021 DEFINITION Vector encoding MCAS(Sync1-MSC16)dsRed virus. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11196) AUTHORS Stafl K, Travnicek M, Kucerova D, Pecnova L, Krchlikova V, Galikova E, Stepanets V, Hejnar J, Trejbalova K TITLE Direct Submission JOURNAL Exported Monday, May 17, 2021 from SnapGene 5.2.5.1 https://www.snapgene.com FEATURES Location/Qualifiers source 1..11196 /organism="recombinant plasmid" /lab_host="Gallus gallus" /mol_type="other DNA" LTR 1..392 misc_feature 393..410 /label=PBS /note="the same as for RCAS" misc_feature 447..607 /label=packaging signal /note="according to Banks JD and Linial ML, J. Virol.,2000" CDS 663..2774 /codon_start=1 /label=gag /note="gag" /translation="MEAVIKVISSACKTYCGKISPSKKEIGAMLSLLQKEGLLMSPSDL YSPGSWDPITAALSQRAMVLGKSGELKTWGLVLGALKAAREEQVTSEQAKFWLGLGGGR VSPPGPECIEKPATERRIDKGEEVGETTVQRDAKMAPEEAATPKTVGTSCYHCGTAVGC NCATATASAPPPPYVGSGLYPSLAGVGEQQGQGDNTSRGAEQPREEPGHAGQAPGPALT DWARVREELASTGPPVVAMPVVIKTEGPAWTPLEPKLITRLADTVRTKGLRSPITMAEV EALMSSPLLPHDVTNLMRVILGPAPYALWMDAWGVQLQTVIAAATRDPRHPANGQGRGE RTNLDRLKGLADGMVGNPQGQAALLRPGELVAITASALQAFREVARLAEPAGPWADITQ GPSESFVDFANRLIKAVEGSDLPPSARAPVIIDCFRQKSQPDIQQLIRAAPSTLTTPGE IIKYVLDRQKIAPLTDQGIAAAMSSAIQPLVMAVVNRERDGQTGSGGRARGLCYTCGSP GHYQAQCPKKRKSGNSRERCQLCDGMGHNAKQCRKRDGNQGQRPGRGLSSGPWPGPEQP AVSLAMTMEHKDRPLVRVILTNTGSHPVKQRSVYITALLDSGADITIISEEDWPTDWPV VDTANPQIHGIGGGIPMRKSRDMIEVGVINRDGSLERPLLLFPAVAMVRGSILGRDCLQ GLGLRLTNL" gene 663..1193 /label=p19 /note="p19" intron 681..682 /label=splice donor /note="splice site (beginning of intron)" gene 1200..1385 /label=p10 /note="p10" gene 1386..2105 /label=p27 /note="p27" gene 2133..2399 /label=p12 /note="p12" gene 2400..2771 /product="protease" /label=p15-protease /note="p15" CDS 2771..5479 /codon_start=1 /label=pol /note="pol" /translation="IGRATVLTVALHLAIPLKWKSDRTPVWIDQWPLPEGKLVALTQLV EKELQLGHIEPSLSCWNTPVFVIRKASGSYRLLHDLRAVNAKLVPFGAVQQGAPVLSAL PRGWPLMVLDLKDCFFSIPLAEQDREAFAFTLPSVNNQAPARRFQWKVLPQGMTCSPTI CQLVVGQVLEPLRLKHPALRMLHYMDDLLLAASSHDGLEAAGKEVIGTLERAGFTISPD KIQREPGVQYLGYKLGSTYVAPVGLVAEPRIATLWDVQKLVGSLQWLRPALGIPPRLMG PFYEQLRGSDPNEAREWNLDMKMAWREIVQLSTTAALERWDPAQPLEGAVARCEQGAIG VLGQGLSTHPRPCLWLFSTQPTKAFTAWLEVLTLLITKLRASAVRTFGKEVDILLLPAC FREDLPLPEGILLALRGFAGKIRSSDTPSIFDIARPLHVSLKVRVTDHPVPGPTVFTDA SSSTHKGVVVWREGPRWEIKEIVDLGASVQQLEARAVAMALLLWPTTPTNVVTDSAFVA KMLLKMGQEGVPSTAAAFILEDALSQRSAMAAVLHVRSHSEVPGFFTEGNDVADSQATF QAYPLREAKDLHTALHIGPRALSKACNISMQQAREVVQTCPHCNSAPALEAGVNPRGLG PLQIWQTDFTLEPRMAPRSWLAVTVDTASSAIVVTQHGRVTSVAAQHHWATAIAVLGRP KAIKTDNGSCFTSRSTREWLARWGIAHTTGIPGNSQGQAMVERANRLLKDKIRVLAEGD GFMKRIPASKQGELLAKAMYALNHFERGENTKTPVQKHWRPTVLTEGPPVKIRIETGEW EKGWNVLVWGRGYAAVKNRDTDKVIWVPSRKVKPDVTQKDEVTKKDEASPLFAGISDWI PWEDEQEGLQGETASNKQERPGEDTLAANES" CDS 2915..3481 /label=reverse transcriptase CDS 4112..4507 /label=RNase H CDS 4664..5155 /label=Integrase intron 5365..5366 /label=splice acceptor /label=splice site /note="splice site (end of intron)" CDS 5367..5534 /codon_start=1 /label=env-sigPE(A) /note="env-sigPE(A)" /translation="AFLTGYPGKTSKKDSKEKPLATSKKDPEKTPLLPTRVNYILIIGV LVLCEVTGVRA" CDS 5535..6923 /codon_start=1 /label=sync1 env /note="sync1 env" /translation="PPCRCMTSSSPYQEFLWRMQRPGNIDAPSYRSLSKGTPTFTAHTH MPRNCYHSATLCMHANTHYWTGKMINPSCPGGLGVTVCWTYFTQTGMSDGGGVQDQARE KHVKEVISQLTRVHGTSSPYKGLDLSKLHETLRTHTRLVSLFNTTLTGLHEVSAQNPTN CWICLPLNFRPYVSIPVPEQWNNFSTEINTTSVLVGPLVSNLEITHTSNLTCVKFSNTT YTTNSQCIRWVTPPTQIVCLPSGIFFVCGTSAYRCLNGSSESMCFLSFLVPPMTIYTEQ DLYSYVISKPRNKRVPILPFVIGAGVLGALGTGIGGITTSTQFYYKLSQELNGDMERVA DSLVTLQDQLNSLAAVVLQNRRALDLLTAERGGTCLFLGEECCYYVNQSGIVTEKVKEI RDRIQRRAEELRNTGPWGLLSQWMPWILPFLGPLAAIILLLLFGPCIFNLLVNFVSSRI EAVKL" CDS 5535..6419 /label=sync1 SU CDS 6420..6923 /label=sync1 TM /note="sync1 TM" misc_structure 6876..6923 /label=cytoplasmic domain /note="cytoplasmic domain" intron 6903..6904 /label=new splice acceptor /note="new splice acceptor in syncytin-1, originally Amutated to c = splice acceptor AG mutated to cG" misc_feature 6924..6926 /label=stop codon introduction intron 7186..7187 /label=splice acceptor CDS 7247..7924 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Strongin et al., 2007)" /label=DsRed-Monomer /note="mammalian codon-optimized" /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS GSQ" LTR 8067..8452 /label=dLTR V_segment complement(8453..11196) /label=pLitmus 28i promoter complement(8508..8526) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(8547..8563) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind 8571..8587 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator toinhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8595..8625) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8640..8661 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presenceof cAMP." rep_origin complement(8722..9310) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" rep_origin 9421..9930 /direction=RIGHT /label=M13 ori /note="M13 bacteriophage origin of replication; arrowindicates direction of (+) strand synthesis" CDS complement(9972..10832) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERSPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(10833..10937) /gene="bla" /label=AmpR promoter primer_bind 11158..11174 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 11178..11196 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" ORIGIN 1 tgtagtctta atcataggtt aacatgtata ttaccaaata agggaatcgc ctgatgcacc 61 aaataaggta ttatatgatc ccattggtgg cgagggagcg acctgagggc atatgggcgt 121 taacagaact gtctgtcctt gcgtcattcc ttcctcatcg gatcatgtac gcggcagagt 181 atgattggat aacaggatgg caccattcat cgtggcgcat gctgattggt gcgactaagg 241 agttatgtaa cccacgaatg tacttaagct tgtagttgct aacaataaag ggccattcta 301 cctctcacca cattggtgtg cacctgggtt gatggccgga ccgtcgattc cctgacgact 361 acgagcacct gaatgaagca gaaggcttca tttggtgacc ccgacgtgat cgttagggaa 421 tagtggtcgg ccacaggcgg cgtggcgatc ctgtctccat ccgtctcgtc gaccgggagg 481 cgagttcgat gaccctgata gagggggctg cggcttaggg aggcagaagc tgagcaccgt 541 cggtaggagc tccagggccc ggagcgactg acccctgccg agaactcaga gggtcgtcgg 601 aagacggaga gtgagcccga cgaccacccc aggcacgtct ttggtcggcc tgcggatcaa 661 gcatggaagc cgtcattaag gtgatttcgt ccgcgtgtaa aacctattgc gggaaaatct 721 ctccttctaa gaaggaaata ggggccatgt tgtccctgtt acaaaaggaa gggttgctta 781 tgtctccctc agatttatat tctccggggt cctgggatcc catcactgcg gcgctctccc 841 agcgggcaat ggtacttgga aaatcgggag agttaaaaac ctggggattg gttttggggg 901 cattgaaggc ggctcgagag gaacaggtta catctgagca agcaaagttt tggttgggat 961 tagggggagg gagggtctct cccccaggtc cggagtgcat cgagaaacca gctacggagc 1021 ggcgaatcga caaaggggag gaggtgggag aaacaactgt gcagcgagat gcgaagatgg 1081 cgccagagga agcggccaca cctaaaaccg ttggcacatc ctgctatcat tgcggaacag 1141 ctgttggctg caattgcgcc accgccacag cctcggcccc tcctccccct tatgtgggga 1201 gtggtttgta tccttccctg gcgggggtgg gagagcagca gggccaggga gataacacgt 1261 ctcggggggc ggagcagcca agggaggagc cagggcacgc gggtcaggcc cctgggccgg 1321 ccctgactga ctgggcaagg gtaagggagg agcttgcgag tactggtccg cccgtggtgg 1381 ccatgcctgt agtgattaag acagagggac ccgcctggac ccctctggag ccaaaattga 1441 tcacaagact ggctgatacg gtcaggacca agggcttacg atccccgatc actatggcag 1501 aagtggaagc gctcatgtcc tccccgttgc tgccgcatga cgtcacgaat ctaatgagag 1561 tgattttagg acctgcccca tatgccttat ggatggacgc ttggggagtc caactccaga 1621 cggttatagc ggcagccact cgcgaccccc gacacccagc gaacggtcaa gggcgggggg 1681 aacggactaa cttggatcga ttaaagggct tagctgatgg gatggtgggc aacccacagg 1741 gtcaggccgc attattaaga ccgggggaat tggttgctat tacggcgtcg gctctccagg 1801 cgtttagaga agttgcccgg ctggcggaac ctgcaggtcc atgggcggac atcacgcagg 1861 gaccatctga gtcctttgtt gattttgcca atcggcttat aaaggcggtt gaggggtcag 1921 atctcccgcc ttccgcgcgg gctccggtga tcattgactg ctttaggcag aagtcacagc 1981 cagatattca gcagcttata cgggcagcac cctccacgct gaccacccca ggagagataa 2041 tcaaatatgt gctagacagg cagaagattg cccctcttac ggatcaaggc atagccgcgg 2101 ccatgtcgtc tgctatccag cccttagtta tggcagtagt caatagagag agggatggac 2161 aaactgggtc gggtggtcgt gcccgagggc tctgctacac ttgtggatcc ccgggacatt 2221 atcaggcaca gtgcccgaaa aaacgaaagt caggaaacag ccgtgagcga tgtcagctgt 2281 gtgacgggat gggacacaac gctaaacagt gtaggaagcg ggatggcaac cagggccaac 2341 gcccaggaag aggtctctct tcggggccgt ggcccggccc tgagcagcct gccgtctcgt 2401 tagcgatgac aatggaacat aaagatcgcc ccttggttag ggtcattctg actaacactg 2461 ggagtcatcc agtcaaacaa cgttcggtgt atatcaccgc gctgttggac tccggagcgg 2521 acatcactat tatttcggag gaggattggc ctactgattg gccggtggtg gacaccgcga 2581 acccacagat ccatggcata ggagggggaa ttcccatgcg aaaatcccgg gatatgatag 2641 aggtgggggt tattaaccga gacgggtcgt tggagcgacc cctgctcctc ttccccgcag 2701 tcgctatggt tagagggagt atcctaggaa gagattgtct gcagggccta gggctccgct 2761 tgacaaattt atagggaggg ccactgttct tactgttgcg ctacatctgg ctattccgct 2821 caaatggaag tcagaccgca cgcctgtgtg gattgaccag tggccccttc ctgaaggtaa 2881 acttgtagcg ctaacgcaat tagtggaaaa agaattacag ttaggacata tagagccctc 2941 acttagttgt tggaacacac ctgtttttgt gatccggaag gcttccgggt cttatcgctt 3001 attgcatgat ttgcgcgctg ttaacgccaa gcttgtccct tttggggccg tccaacaggg 3061 ggcgccagtt ctctccgcgc tcccgcgtgg ctggcccctg atggtcctag acctcaagga 3121 ttgcttcttt tctatccctc ttgcggaaca agatcgcgaa gcttttgcat ttacgctccc 3181 ctctgtgaat aaccaggccc ccgctcgaag attccaatgg aaggtcttgc cccaagggat 3241 gacctgttct cccactatct gtcagttggt agtgggtcag gtgctcgagc ccttgcgact 3301 caagcaccca gctctgcgca tgttgcatta tatggacgat cttttgctag ccgcctcaag 3361 tcatgatggg ttggaagcgg cagggaagga ggttatcggt acattggaaa gagccgggtt 3421 cactatttcg ccggataaga tccagaggga gcccggagta caatatcttg ggtacaagtt 3481 aggcagtacg tatgtagcac ccgtaggctt ggtagcagaa cccaggatag ccaccttgtg 3541 ggatgttcaa aagctggtgg ggtcacttca gtggcttcgc ccagcgttag ggatcccgcc 3601 acgactgatg ggtccctttt atgagcagtt acgagggtca gatcctaacg aggcgaggga 3661 atggaatcta gacatgaaaa tggcctggag agagatcgta cagcttagca ctactgctgc 3721 cttggaacga tgggaccctg cccagcctct ggaaggagcg gtcgctagat gtgaacaggg 3781 ggcaataggg gtcctgggac agggactgtc cacacaccca aggccatgtt tgtggttatt 3841 ctccacccaa cccaccaagg cgtttactgc ttggttagaa gtgctcaccc ttttgattac 3901 taagctacgc gcttcggcag tgcgaacctt tggcaaggag gttgatatcc tcctgttgcc 3961 tgcatgcttc cgggaggacc ttccgctccc ggaggggatc ctgttagcac ttagggggtt 4021 tgcaggaaaa atcaggagta gtgacacgcc atctattttt gacattgcgc gtccactgca 4081 tgtttctctg aaagtgaggg ttaccgacca ccctgtgccg ggacccactg tctttaccga 4141 cgcctcctca agcacccata aaggggtggt agtctggagg gagggcccaa ggtgggagat 4201 aaaagaaata gttgatttgg gggcaagtgt acaacaactg gaggcacgcg ctgtggccat 4261 ggcacttctg ctgtggccga caacgcccac taatgtagtg actgactctg cgtttgttgc 4321 gaaaatgtta ctcaagatgg gacaggaggg agtcccgtct acagcggcag cttttatttt 4381 agaggatgcg ttaagccaaa ggtcagccat ggccgccgtt ctccacgtgc ggagtcattc 4441 tgaagtgcca gggtttttca cagaaggaaa tgacgtggca gatagccaag ccacctttca 4501 agcgtatccc ttgagagagg ctaaagatct tcataccgct ctccatattg gaccccgcgc 4561 gctatccaaa gcgtgtaata tatctatgca gcaggctagg gaggttgttc agacctgccc 4621 gcattgtaat tcagcccctg cgttggaggc cggggtaaac cctaggggtt tgggacccct 4681 acagatatgg cagacagact ttacgcttga gcctagaatg gctccccgtt cctggctcgc 4741 tgttactgtg gacaccgcct catcagcgat agtcgtaact cagcatggcc gtgttacatc 4801 ggttgctgca caacatcatt gggccacggc tatcgccgtt ttgggaagac caaaggccat 4861 aaaaacagat aacgggtcct gcttcacgtc tagatccacg cgggagtggc tcgcgagatg 4921 ggggatagca cacaccaccg ggattccggg aaattcccag ggtcaagcta tggtagagcg 4981 ggccaaccgg ctcctgaaag ataagatccg tgtgcttgcg gagggggacg gctttatgaa 5041 aagaatcccc gccagcaaac agggggaact actagccaag gcaatgtatg ccctcaatca 5101 ctttgagcgt ggtgaaaaca caaaaacacc ggtacaaaaa cactggagac ctaccgttct 5161 tacagaagga cccccggtta aaatacgaat agagacaggg gagtgggaaa aaggatggaa 5221 cgtgctggtc tggggacgag gttatgccgc tgtgaaaaac agggacactg ataaggttat 5281 ttgggtaccc tctcggaaag ttaaaccgga tgtcacccaa aaggatgagg tgactaagaa 5341 agatgaggcg agccctcttt ttgcaggcat ttctgactgg ataccctggg aagacgagca 5401 agaaggactc caaggagaaa ccgctagcaa caagcaagaa agacccggag aagacaccct 5461 tgctgccaac gagagttaat tatattctca ttattggtgt cctggtcttg tgtgaggtta 5521 cgggggtaag agctcctcca tgccgctgta tgaccagtag ctccccttac caagagtttc 5581 tatggagaat gcagcgtccc ggaaatattg atgccccatc gtataggagt ctttctaagg 5641 gaacccccac cttcactgcc cacacccata tgccccgcaa ctgctatcac tctgccactc 5701 tttgcatgca tgcaaatact cattattgga caggaaaaat gattaatcct agttgtcctg 5761 gaggacttgg agtcactgtc tgttggactt acttcaccca aactggtatg tctgatgggg 5821 gtggagttca agatcaggca agagaaaaac atgtaaaaga agtaatctcc caactcaccc 5881 gggtacatgg cacctctagc ccctacaaag gactagatct ctcaaaacta catgaaaccc 5941 tccgtaccca tactcgcctg gtaagcctat ttaataccac cctcactggg ctccatgagg 6001 tctcggccca aaaccctact aactgttgga tatgcctccc cctgaacttc aggccatatg 6061 tttcaatccc tgtacctgaa caatggaaca acttcagcac agaaataaac accacttccg 6121 ttttagtagg acctcttgtt tccaatctgg aaataaccca tacctcaaac ctcacctgtg 6181 taaaatttag caatactaca tacacaacca actcccaatg catcaggtgg gtaactcctc 6241 ccacacaaat agtctgccta ccctcaggaa tattttttgt ctgtggaacc tcagcctatc 6301 gttgtttgaa tggctcttca gaatctatgt gcttcctctc attcttagtg ccccctatga 6361 ccatctacac tgaacaagat ttatacagtt atgtcatatc taagccccgc aacaaaaggg 6421 tacccattct tccttttgtt ataggagcag gagtgctagg tgcactaggt actggcattg 6481 gcggtatcac aacctctact cagttctact acaaactatc tcaagaacta aatggggaca 6541 tggaacgggt cgccgactcc ctggtcacct tgcaagatca acttaactcc ctagcagcag 6601 tagtccttca aaatcgaaga gctttagact tgctaaccgc tgaaagaggg ggaacctgtt 6661 tatttttagg ggaagaatgc tgttattatg ttaatcaatc cggaatcgtc actgagaaag 6721 ttaaagaaat tcgagatcga atacaacgta gagcagagga gcttcgaaac actggaccct 6781 ggggcctcct cagccaatgg atgccctgga ttctcccctt cttaggacct ctagcagcta 6841 taatattgct actcctcttt ggaccctgta tctttaacct ccttgttaac tttgtctctt 6901 cccgaatcga agctgtaaaa ctataattgg agcccaagat gcagtccaag actaagatct 6961 accgcagacc cctggaccgg cctgctagcc cacgatctga tgttaatgac atcaaaggca 7021 cccctcctga ggaaatctca gctgcacaac ctctactacg ccccaattca gcaggaagca 7081 gttagggcag tacatgggtg gtggtatagc gcttgcgagt ccatcgagca aggcaggaaa 7141 gacagctatt ggtaattgtg aaatacgctt ttgtctgtgt gctgcaggag ctgagctgac 7201 tctgctggtg gcctcgcgta ccactgtggc atcgatggtc gccaccatgg acaacaccga 7261 ggacgtcatc aaggagttca tgcagttcaa ggtgcgcatg gagggctccg tgaacggcca 7321 ctacttcgag atcgagggcg agggcgaggg caagccctac gagggcaccc agaccgccaa 7381 gctgcaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt 7441 ccagtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acatgaagct 7501 gtccttcccc gagggcttca cctgggagcg ctccatgaac ttcgaggacg gcggcgtggt 7561 ggaggtgcag caggactcct ccctgcagga cggcaccttc atctacaagg tgaagttcaa 7621 gggcgtgaac ttccccgccg acggccccgt aatgcagaag aagactgccg gctgggagcc 7681 ctccaccgag aagctgtacc cccaggacgg cgtgctgaag ggcgagatct cccacgccct 7741 gaagctgaag gacggcggcc actacacctg cgacttcaag accgtgtaca aggccaagaa 7801 gcccgtgcag ctgcccggca accactacgt ggactccaag ctggacatca ccaaccacaa 7861 cgaggactac accgtggtgg agcagtacga gcacgccgag gcccgccact ccggctccca 7921 gtagagatcg atgcgatgta cgggccagat atacgcgtat ctgaggggac tagggtgtgt 7981 ttaggcgaaa agcggggctt cggttgtacg cggttaggag tcccctcgag gtatggcaga 8041 tatgcttttg catagggagg gggaaatgta gtcttaatca taggttaaca tgtatattac 8101 caaataaggg aatcgcctga tgcaccaaat aaggtattat atgatcccat tggtggcgag 8161 ggagcgacct gagggcatat gggcgttaac agaactgtct gtccttgcgt cattccttcc 8221 tcatcggatc atgtacgcgg cagagtatga ttggataaca ggatggcacc attcatcgtg 8281 gcgcatgctg attggtgcga ctaaggagtt atgtaaccca cgaatgtact taagcttgta 8341 gttgctaaca ataaagggcc attctacctc tcaccacatt ggtgtgcacc tgggttgatg 8401 gccggaccgt cgattccctg acgactacga gcacctgaat gaagcagaag gcatcctgca 8461 ggaattcctc gagacgtacg gtgcgcgcga tgcattcgaa gatctgccct atagtgagtc 8521 gtattacgta gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg 8581 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa 8641 tgagtgagct aactcacatt acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 8701 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 8761 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 8821 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 8881 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 8941 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 9001 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 9061 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 9121 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg 9181 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 9241 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 9301 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 9361 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 9421 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 9481 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 9541 cgttcgcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc 9601 tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc 9661 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact 9721 cttgttccaa actggaacaa cactcaaccc tatctcgggc tattcttttg atttataagg 9781 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc 9841 gaattttaac aaaatattaa cgtttacaat ttaaatattt gcttatacaa tcttcctgtt 9901 tttggggctt ttctgattat caaccggggt aaatcaatct aaagtatata tgagtaaact 9961 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 10021 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 10081 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 10141 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 10201 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 10261 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 10321 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 10381 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 10441 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 10501 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 10561 cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact 10621 ttaaaagtgc tcatcattgg agaacgttct tcggggcgaa aactctcaag gatcttaccg 10681 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 10741 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 10801 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 10861 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 10921 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtagt taacaaaaaa 10981 aagcccgccg aagcgggctt tattaccaag cgaagcgcca ttcgccattc aggctgcgca 11041 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg 11101 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta 11161 aaacgacggc cagtccgtaa tacgactcac tatagg //