LOCUS Exported 11262 bp ds-DNA circular SYN 17-MAY-2021 DEFINITION Vector expressing Syncytin-1 used for luminescence assay. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11262) AUTHORS Stafl K, Travnicek M, Kucerova D, Pecnova L, Krchlikova V, Galikova E, Stepanets V, Hejnar J, Trejbalova K TITLE Direct Submission JOURNAL Exported Monday, May 17, 2021 from SnapGene 5.2.5.1 https://www.snapgene.com FEATURES Location/Qualifiers source 1..11262 /organism="recombinant plasmid" /lab_host="Gallus gallus" /mol_type="other DNA" LTR 1..392 misc_feature 393..410 /label=PBS /note="the same as for RCAS" misc_feature 447..607 /label=packaging signal /note="according to Banks JD and Linial ML, J. Virol.,2000" CDS 663..2774 /codon_start=1 /label=gag /note="gag" /translation="MEAVIKVISSACKTYCGKISPSKKEIGAMLSLLQKEGLLMSPSDL YSPGSWDPITAALSQRAMVLGKSGELKTWGLVLGALKAAREEQVTSEQAKFWLGLGGGR VSPPGPECIEKPATERRIDKGEEVGETTVQRDAKMAPEEAATPKTVGTSCYHCGTAVGC NCATATASAPPPPYVGSGLYPSLAGVGEQQGQGDNTSRGAEQPREEPGHAGQAPGPALT DWARVREELASTGPPVVAMPVVIKTEGPAWTPLEPKLITRLADTVRTKGLRSPITMAEV EALMSSPLLPHDVTNLMRVILGPAPYALWMDAWGVQLQTVIAAATRDPRHPANGQGRGE RTNLDRLKGLADGMVGNPQGQAALLRPGELVAITASALQAFREVARLAEPAGPWADITQ GPSESFVDFANRLIKAVEGSDLPPSARAPVIIDCFRQKSQPDIQQLIRAAPSTLTTPGE IIKYVLDRQKIAPLTDQGIAAAMSSAIQPLVMAVVNRERDGQTGSGGRARGLCYTCGSP GHYQAQCPKKRKSGNSRERCQLCDGMGHNAKQCRKRDGNQGQRPGRGLSSGPWPGPEQP AVSLAMTMEHKDRPLVRVILTNTGSHPVKQRSVYITALLDSGADITIISEEDWPTDWPV VDTANPQIHGIGGGIPMRKSRDMIEVGVINRDGSLERPLLLFPAVAMVRGSILGRDCLQ GLGLRLTNL" gene 663..1193 /label=p19 /note="p19" intron 681..682 /label=splice donor /note="splice site (beginning of intron)" gene 1200..1385 /label=p10 /note="p10" gene 1386..2105 /label=p27 /note="p27" gene 2133..2399 /label=p12 /note="p12" gene 2400..2771 /product="protease" /label=p15-protease /note="p15" CDS 2771..5479 /codon_start=1 /label=pol /note="pol" /translation="IGRATVLTVALHLAIPLKWKSDRTPVWIDQWPLPEGKLVALTQLV EKELQLGHIEPSLSCWNTPVFVIRKASGSYRLLHDLRAVNAKLVPFGAVQQGAPVLSAL PRGWPLMVLDLKDCFFSIPLAEQDREAFAFTLPSVNNQAPARRFQWKVLPQGMTCSPTI CQLVVGQVLEPLRLKHPALRMLHYMDDLLLAASSHDGLEAAGKEVIGTLERAGFTISPD KIQREPGVQYLGYKLGSTYVAPVGLVAEPRIATLWDVQKLVGSLQWLRPALGIPPRLMG PFYEQLRGSDPNEAREWNLDMKMAWREIVQLSTTAALERWDPAQPLEGAVARCEQGAIG VLGQGLSTHPRPCLWLFSTQPTKAFTAWLEVLTLLITKLRASAVRTFGKEVDILLLPAC FREDLPLPEGILLALRGFAGKIRSSDTPSIFDIARPLHVSLKVRVTDHPVPGPTVFTDA SSSTHKGVVVWREGPRWEIKEIVDLGASVQQLEARAVAMALLLWPTTPTNVVTDSAFVA KMLLKMGQEGVPSTAAAFILEDALSQRSAMAAVLHVRSHSEVPGFFTEGNDVADSQATF QAYPLREAKDLHTALHIGPRALSKACNISMQQAREVVQTCPHCNSAPALEAGVNPRGLG PLQIWQTDFTLEPRMAPRSWLAVTVDTASSAIVVTQHGRVTSVAAQHHWATAIAVLGRP KAIKTDNGSCFTSRSTREWLARWGIAHTTGIPGNSQGQAMVERANRLLKDKIRVLAEGD GFMKRIPASKQGELLAKAMYALNHFERGENTKTPVQKHWRPTVLTEGPPVKIRIETGEW EKGWNVLVWGRGYAAVKNRDTDKVIWVPSRKVKPDVTQKDEVTKKDEASPLFAGISDWI PWEDEQEGLQGETASNKQERPGEDTLAANES" CDS 2915..3481 /label=reverse transcriptase CDS 4112..4507 /label=RNase H CDS 4664..5155 /label=Integrase intron 5365..5366 /label=splice acceptor /label=splice site /note="splice site (end of intron)" CDS 5367..5534 /codon_start=1 /label=env-sigPE(A) /note="env-sigPE(A)" /translation="AFLTGYPGKTSKKDSKEKPLATSKKDPEKTPLLPTRVNYILIIGV LVLCEVTGVRA" CDS 5535..5600 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" CDS 5601..7151 /codon_start=1 /label=sync1 env /note="sync1 env" /translation="PPCRCMTSSSPYQEFLWRMQRPGNIDAPSYRSLSKGTPTFTAHTH MPRNCYHSATLCMHANTHYWTGKMINPSCPGGLGVTVCWTYFTQTGMSDGGGVQDQARE KHVKEVISQLTRVHGTSSPYKGLDLSKLHETLRTHTRLVSLFNTTLTGLHEVSAQNPTN CWICLPLNFRPYVSIPVPEQWNNFSTEINTTSVLVGPLVSNLEITHTSNLTCVKFSNTT YTTNSQCIRWVTPPTQIVCLPSGIFFVCGTSAYRCLNGSSESMCFLSFLVPPMTIYTEQ DLYSYVISKPRNKRVPILPFVIGAGVLGALGTGIGGITTSTQFYYKLSQELNGDMERVA DSLVTLQDQLNSLAAVVLQNRRALDLLTAERGGTCLFLGEECCYYVNQSGIVTEKVKEI RDRIQRRAEELRNTGPWGLLSQWMPWILPFLGPLAAIILLLLFGPCIFNLLVNFVSSRI EAVKLQMEPKMQSKTKIYRRPLDRPASPRSDVNDIKGTPPEEISAAQPLLRPNSAGSS" CDS 5601..6485 /label=sync1 SU CDS 6486..7151 /label=sync1 TM /note="sync1 TM" misc_structure 6942..7148 /label=cytoplasmic domain /note="cytoplasmic domain" intron 6969..6970 /label=new splice acceptor /note="new splice acceptor in syncytin-1, originallyAmutated to c = splice acceptor AG mutated to cG" intron 7252..7253 /label=splice acceptor CDS 7313..7990 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Strongin et al., 2007)" /label=DsRed-Monomer /note="mammalian codon-optimized" /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS GSQ" LTR 8133..8518 /label=dLTR V_segment complement(8519..11262) /label=pLitmus 28i promoter complement(8574..8592) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(8613..8629) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind 8637..8653 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operatortoinhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8661..8691) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8706..8727 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in thepresenceof cAMP." rep_origin complement(8788..9376) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" rep_origin 9487..9996 /direction=RIGHT /label=M13 ori /note="M13 bacteriophage origin of replication;arrowindicates direction of (+) strand synthesis" CDS complement(10038..10898) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERSPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(10899..11003) /gene="bla" /label=AmpR promoter primer_bind 11224..11240 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 11244..11262 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" ORIGIN 1 tgtagtctta atcataggtt aacatgtata ttaccaaata agggaatcgc ctgatgcacc 61 aaataaggta ttatatgatc ccattggtgg cgagggagcg acctgagggc atatgggcgt 121 taacagaact gtctgtcctt gcgtcattcc ttcctcatcg gatcatgtac gcggcagagt 181 atgattggat aacaggatgg caccattcat cgtggcgcat gctgattggt gcgactaagg 241 agttatgtaa cccacgaatg tacttaagct tgtagttgct aacaataaag ggccattcta 301 cctctcacca cattggtgtg cacctgggtt gatggccgga ccgtcgattc cctgacgact 361 acgagcacct gaatgaagca gaaggcttca tttggtgacc ccgacgtgat cgttagggaa 421 tagtggtcgg ccacaggcgg cgtggcgatc ctgtctccat ccgtctcgtc gaccgggagg 481 cgagttcgat gaccctgata gagggggctg cggcttaggg aggcagaagc tgagcaccgt 541 cggtaggagc tccagggccc ggagcgactg acccctgccg agaactcaga gggtcgtcgg 601 aagacggaga gtgagcccga cgaccacccc aggcacgtct ttggtcggcc tgcggatcaa 661 gcatggaagc cgtcattaag gtgatttcgt ccgcgtgtaa aacctattgc gggaaaatct 721 ctccttctaa gaaggaaata ggggccatgt tgtccctgtt acaaaaggaa gggttgctta 781 tgtctccctc agatttatat tctccggggt cctgggatcc catcactgcg gcgctctccc 841 agcgggcaat ggtacttgga aaatcgggag agttaaaaac ctggggattg gttttggggg 901 cattgaaggc ggctcgagag gaacaggtta catctgagca agcaaagttt tggttgggat 961 tagggggagg gagggtctct cccccaggtc cggagtgcat cgagaaacca gctacggagc 1021 ggcgaatcga caaaggggag gaggtgggag aaacaactgt gcagcgagat gcgaagatgg 1081 cgccagagga agcggccaca cctaaaaccg ttggcacatc ctgctatcat tgcggaacag 1141 ctgttggctg caattgcgcc accgccacag cctcggcccc tcctccccct tatgtgggga 1201 gtggtttgta tccttccctg gcgggggtgg gagagcagca gggccaggga gataacacgt 1261 ctcggggggc ggagcagcca agggaggagc cagggcacgc gggtcaggcc cctgggccgg 1321 ccctgactga ctgggcaagg gtaagggagg agcttgcgag tactggtccg cccgtggtgg 1381 ccatgcctgt agtgattaag acagagggac ccgcctggac ccctctggag ccaaaattga 1441 tcacaagact ggctgatacg gtcaggacca agggcttacg atccccgatc actatggcag 1501 aagtggaagc gctcatgtcc tccccgttgc tgccgcatga cgtcacgaat ctaatgagag 1561 tgattttagg acctgcccca tatgccttat ggatggacgc ttggggagtc caactccaga 1621 cggttatagc ggcagccact cgcgaccccc gacacccagc gaacggtcaa gggcgggggg 1681 aacggactaa cttggatcga ttaaagggct tagctgatgg gatggtgggc aacccacagg 1741 gtcaggccgc attattaaga ccgggggaat tggttgctat tacggcgtcg gctctccagg 1801 cgtttagaga agttgcccgg ctggcggaac ctgcaggtcc atgggcggac atcacgcagg 1861 gaccatctga gtcctttgtt gattttgcca atcggcttat aaaggcggtt gaggggtcag 1921 atctcccgcc ttccgcgcgg gctccggtga tcattgactg ctttaggcag aagtcacagc 1981 cagatattca gcagcttata cgggcagcac cctccacgct gaccacccca ggagagataa 2041 tcaaatatgt gctagacagg cagaagattg cccctcttac ggatcaaggc atagccgcgg 2101 ccatgtcgtc tgctatccag cccttagtta tggcagtagt caatagagag agggatggac 2161 aaactgggtc gggtggtcgt gcccgagggc tctgctacac ttgtggatcc ccgggacatt 2221 atcaggcaca gtgcccgaaa aaacgaaagt caggaaacag ccgtgagcga tgtcagctgt 2281 gtgacgggat gggacacaac gctaaacagt gtaggaagcg ggatggcaac cagggccaac 2341 gcccaggaag aggtctctct tcggggccgt ggcccggccc tgagcagcct gccgtctcgt 2401 tagcgatgac aatggaacat aaagatcgcc ccttggttag ggtcattctg actaacactg 2461 ggagtcatcc agtcaaacaa cgttcggtgt atatcaccgc gctgttggac tccggagcgg 2521 acatcactat tatttcggag gaggattggc ctactgattg gccggtggtg gacaccgcga 2581 acccacagat ccatggcata ggagggggaa ttcccatgcg aaaatcccgg gatatgatag 2641 aggtgggggt tattaaccga gacgggtcgt tggagcgacc cctgctcctc ttccccgcag 2701 tcgctatggt tagagggagt atcctaggaa gagattgtct gcagggccta gggctccgct 2761 tgacaaattt atagggaggg ccactgttct tactgttgcg ctacatctgg ctattccgct 2821 caaatggaag tcagaccgca cgcctgtgtg gattgaccag tggccccttc ctgaaggtaa 2881 acttgtagcg ctaacgcaat tagtggaaaa agaattacag ttaggacata tagagccctc 2941 acttagttgt tggaacacac ctgtttttgt gatccggaag gcttccgggt cttatcgctt 3001 attgcatgat ttgcgcgctg ttaacgccaa gcttgtccct tttggggccg tccaacaggg 3061 ggcgccagtt ctctccgcgc tcccgcgtgg ctggcccctg atggtcctag acctcaagga 3121 ttgcttcttt tctatccctc ttgcggaaca agatcgcgaa gcttttgcat ttacgctccc 3181 ctctgtgaat aaccaggccc ccgctcgaag attccaatgg aaggtcttgc cccaagggat 3241 gacctgttct cccactatct gtcagttggt agtgggtcag gtgctcgagc ccttgcgact 3301 caagcaccca gctctgcgca tgttgcatta tatggacgat cttttgctag ccgcctcaag 3361 tcatgatggg ttggaagcgg cagggaagga ggttatcggt acattggaaa gagccgggtt 3421 cactatttcg ccggataaga tccagaggga gcccggagta caatatcttg ggtacaagtt 3481 aggcagtacg tatgtagcac ccgtaggctt ggtagcagaa cccaggatag ccaccttgtg 3541 ggatgttcaa aagctggtgg ggtcacttca gtggcttcgc ccagcgttag ggatcccgcc 3601 acgactgatg ggtccctttt atgagcagtt acgagggtca gatcctaacg aggcgaggga 3661 atggaatcta gacatgaaaa tggcctggag agagatcgta cagcttagca ctactgctgc 3721 cttggaacga tgggaccctg cccagcctct ggaaggagcg gtcgctagat gtgaacaggg 3781 ggcaataggg gtcctgggac agggactgtc cacacaccca aggccatgtt tgtggttatt 3841 ctccacccaa cccaccaagg cgtttactgc ttggttagaa gtgctcaccc ttttgattac 3901 taagctacgc gcttcggcag tgcgaacctt tggcaaggag gttgatatcc tcctgttgcc 3961 tgcatgcttc cgggaggacc ttccgctccc ggaggggatc ctgttagcac ttagggggtt 4021 tgcaggaaaa atcaggagta gtgacacgcc atctattttt gacattgcgc gtccactgca 4081 tgtttctctg aaagtgaggg ttaccgacca ccctgtgccg ggacccactg tctttaccga 4141 cgcctcctca agcacccata aaggggtggt agtctggagg gagggcccaa ggtgggagat 4201 aaaagaaata gttgatttgg gggcaagtgt acaacaactg gaggcacgcg ctgtggccat 4261 ggcacttctg ctgtggccga caacgcccac taatgtagtg actgactctg cgtttgttgc 4321 gaaaatgtta ctcaagatgg gacaggaggg agtcccgtct acagcggcag cttttatttt 4381 agaggatgcg ttaagccaaa ggtcagccat ggccgccgtt ctccacgtgc ggagtcattc 4441 tgaagtgcca gggtttttca cagaaggaaa tgacgtggca gatagccaag ccacctttca 4501 agcgtatccc ttgagagagg ctaaagatct tcataccgct ctccatattg gaccccgcgc 4561 gctatccaaa gcgtgtaata tatctatgca gcaggctagg gaggttgttc agacctgccc 4621 gcattgtaat tcagcccctg cgttggaggc cggggtaaac cctaggggtt tgggacccct 4681 acagatatgg cagacagact ttacgcttga gcctagaatg gctccccgtt cctggctcgc 4741 tgttactgtg gacaccgcct catcagcgat agtcgtaact cagcatggcc gtgttacatc 4801 ggttgctgca caacatcatt gggccacggc tatcgccgtt ttgggaagac caaaggccat 4861 aaaaacagat aacgggtcct gcttcacgtc tagatccacg cgggagtggc tcgcgagatg 4921 ggggatagca cacaccaccg ggattccggg aaattcccag ggtcaagcta tggtagagcg 4981 ggccaaccgg ctcctgaaag ataagatccg tgtgcttgcg gagggggacg gctttatgaa 5041 aagaatcccc gccagcaaac agggggaact actagccaag gcaatgtatg ccctcaatca 5101 ctttgagcgt ggtgaaaaca caaaaacacc ggtacaaaaa cactggagac ctaccgttct 5161 tacagaagga cccccggtta aaatacgaat agagacaggg gagtgggaaa aaggatggaa 5221 cgtgctggtc tggggacgag gttatgccgc tgtgaaaaac agggacactg ataaggttat 5281 ttgggtaccc tctcggaaag ttaaaccgga tgtcacccaa aaggatgagg tgactaagaa 5341 agatgaggcg agccctcttt ttgcaggcat ttctgactgg ataccctggg aagacgagca 5401 agaaggactc caaggagaaa ccgctagcaa caagcaagaa agacccggag aagacaccct 5461 tgctgccaac gagagttaat tatattctca ttattggtgt cctggtcttg tgtgaggtta 5521 cgggggtaag agctgactac aaagatcatg atggtgatta taaggatcat gacatcgact 5581 acaaagacga tgacgacaag cctccatgcc gctgtatgac cagtagctcc ccttaccaag 5641 agtttctatg gagaatgcag cgtcccggaa atattgatgc cccatcgtat aggagtcttt 5701 ctaagggaac ccccaccttc actgcccaca cccatatgcc ccgcaactgc tatcactctg 5761 ccactctttg catgcatgca aatactcatt attggacagg aaaaatgatt aatcctagtt 5821 gtcctggagg acttggagtc actgtctgtt ggacttactt cacccaaact ggtatgtctg 5881 atgggggtgg agttcaagat caggcaagag aaaaacatgt aaaagaagta atctcccaac 5941 tcacccgggt acatggcacc tctagcccct acaaaggact agatctctca aaactacatg 6001 aaaccctccg tacccatact cgcctggtaa gcctatttaa taccaccctc actgggctcc 6061 atgaggtctc ggcccaaaac cctactaact gttggatatg cctccccctg aacttcaggc 6121 catatgtttc aatccctgta cctgaacaat ggaacaactt cagcacagaa ataaacacca 6181 cttccgtttt agtaggacct cttgtttcca atctggaaat aacccatacc tcaaacctca 6241 cctgtgtaaa atttagcaat actacataca caaccaactc ccaatgcatc aggtgggtaa 6301 ctcctcccac acaaatagtc tgcctaccct caggaatatt ttttgtctgt ggaacctcag 6361 cctatcgttg tttgaatggc tcttcagaat ctatgtgctt cctctcattc ttagtgcccc 6421 ctatgaccat ctacactgaa caagatttat acagttatgt catatctaag ccccgcaaca 6481 aaagggtacc cattcttcct tttgttatag gagcaggagt gctaggtgca ctaggtactg 6541 gcattggcgg tatcacaacc tctactcagt tctactacaa actatctcaa gaactaaatg 6601 gggacatgga acgggtcgcc gactccctgg tcaccttgca agatcaactt aactccctag 6661 cagcagtagt ccttcaaaat cgaagagctt tagacttgct aaccgctgaa agagggggaa 6721 cctgtttatt tttaggggaa gaatgctgtt attatgttaa tcaatccgga atcgtcactg 6781 agaaagttaa agaaattcga gatcgaatac aacgtagagc agaggagctt cgaaacactg 6841 gaccctgggg cctcctcagc caatggatgc cctggattct ccccttctta ggacctctag 6901 cagctataat attgctactc ctctttggac cctgtatctt taacctcctt gttaactttg 6961 tctcttcccg aatcgaagct gtaaaactac aaatggagcc caagatgcag tccaagacta 7021 agatctaccg cagacccctg gaccggcctg ctagcccacg atctgatgtt aatgacatca 7081 aaggcacccc tcctgaggaa atctcagctg cacaacctct actacgcccc aattcagcag 7141 gaagcagtta gggcagtaca tgggtggtgg tatagcgctt gcgagtccat cgagcaaggc 7201 aggaaagaca gctattggta attgtgaaat acgcttttgt ctgtgtgctg caggagctga 7261 gctgactctg ctggtggcct cgcgtaccac tgtggcatcg atggtcgcca ccatggacaa 7321 caccgaggac gtcatcaagg agttcatgca gttcaaggtg cgcatggagg gctccgtgaa 7381 cggccactac ttcgagatcg agggcgaggg cgagggcaag ccctacgagg gcacccagac 7441 cgccaagctg caggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc 7501 ccagttccag tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactacat 7561 gaagctgtcc ttccccgagg gcttcacctg ggagcgctcc atgaacttcg aggacggcgg 7621 cgtggtggag gtgcagcagg actcctccct gcaggacggc accttcatct acaaggtgaa 7681 gttcaagggc gtgaacttcc ccgccgacgg ccccgtaatg cagaagaaga ctgccggctg 7741 ggagccctcc accgagaagc tgtaccccca ggacggcgtg ctgaagggcg agatctccca 7801 cgccctgaag ctgaaggacg gcggccacta cacctgcgac ttcaagaccg tgtacaaggc 7861 caagaagccc gtgcagctgc ccggcaacca ctacgtggac tccaagctgg acatcaccaa 7921 ccacaacgag gactacaccg tggtggagca gtacgagcac gccgaggccc gccactccgg 7981 ctcccagtag agatcgatgc gatgtacggg ccagatatac gcgtatctga ggggactagg 8041 gtgtgtttag gcgaaaagcg gggcttcggt tgtacgcggt taggagtccc ctcgaggtat 8101 ggcagatatg cttttgcata gggaggggga aatgtagtct taatcatagg ttaacatgta 8161 tattaccaaa taagggaatc gcctgatgca ccaaataagg tattatatga tcccattggt 8221 ggcgagggag cgacctgagg gcatatgggc gttaacagaa ctgtctgtcc ttgcgtcatt 8281 ccttcctcat cggatcatgt acgcggcaga gtatgattgg ataacaggat ggcaccattc 8341 atcgtggcgc atgctgattg gtgcgactaa ggagttatgt aacccacgaa tgtacttaag 8401 cttgtagttg ctaacaataa agggccattc tacctctcac cacattggtg tgcacctggg 8461 ttgatggccg gaccgtcgat tccctgacga ctacgagcac ctgaatgaag cagaaggcat 8521 cctgcaggaa ttcctcgaga cgtacggtgc gcgcgatgca ttcgaagatc tgccctatag 8581 tgagtcgtat tacgtagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 8641 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt 8701 gcctaatgag tgagctaact cacattacat gtgagcaaaa ggccagcaaa aggccaggaa 8761 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 8821 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 8881 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 8941 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 9001 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 9061 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 9121 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 9181 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg 9241 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 9301 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 9361 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 9421 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 9481 ccttttacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg 9541 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc 9601 tcgccacgtt cgctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt 9661 tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg 9721 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag 9781 tggactcttg ttccaaactg gaacaacact caaccctatc tcgggctatt cttttgattt 9841 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt 9901 taacgcgaat tttaacaaaa tattaacgtt tacaatttaa atatttgctt atacaatctt 9961 cctgtttttg gggcttttct gattatcaac cggggtaaat caatctaaag tatatatgag 10021 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 10081 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 10141 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 10201 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 10261 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 10321 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 10381 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 10441 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 10501 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 10561 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 10621 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc 10681 agaactttaa aagtgctcat cattggagaa cgttcttcgg ggcgaaaact ctcaaggatc 10741 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 10801 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 10861 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 10921 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 10981 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtagttaac 11041 aaaaaaaagc ccgccgaagc gggctttatt accaagcgaa gcgccattcg ccattcaggc 11101 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga 11161 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac 11221 gttgtaaaac gacggccagt ccgtaatacg actcactata gg //