LOCUS Exported 11313 bp ds-DNA circular SYN 17-MAY-2021 DEFINITION Vector expressing MCAS(E)dsRed virus. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11313) AUTHORS Stafl K, Travnicek M, Kucerova D, Pecnova L, Krchlikova V, Galikova E, Stepanets V, Hejnar J, Trejbalova K TITLE Direct Submission JOURNAL Exported Monday, May 17, 2021 from SnapGene 5.2.5.1 https://www.snapgene.com FEATURES Location/Qualifiers source 1..11313 /organism="recombinant plasmid" /lab_host="Gallus gallus" /mol_type="other DNA" LTR 1..392 misc_feature 393..410 /label=PBS /note="the same as for RCAS" misc_feature 447..607 /label=packaging signal /note="according to Banks JD and Linial ML, J. Virol.,2000" CDS 663..2774 /codon_start=1 /label=gag /note="gag" /translation="MEAVIKVISSACKTYCGKISPSKKEIGAMLSLLQKEGLLMSPSDL YSPGSWDPITAALSQRAMVLGKSGELKTWGLVLGALKAAREEQVTSEQAKFWLGLGGGR VSPPGPECIEKPATERRIDKGEEVGETTVQRDAKMAPEEAATPKTVGTSCYHCGTAVGC NCATATASAPPPPYVGSGLYPSLAGVGEQQGQGDNTSRGAEQPREEPGHAGQAPGPALT DWARVREELASTGPPVVAMPVVIKTEGPAWTPLEPKLITRLADTVRTKGLRSPITMAEV EALMSSPLLPHDVTNLMRVILGPAPYALWMDAWGVQLQTVIAAATRDPRHPANGQGRGE RTNLDRLKGLADGMVGNPQGQAALLRPGELVAITASALQAFREVARLAEPAGPWADITQ GPSESFVDFANRLIKAVEGSDLPPSARAPVIIDCFRQKSQPDIQQLIRAAPSTLTTPGE IIKYVLDRQKIAPLTDQGIAAAMSSAIQPLVMAVVNRERDGQTGSGGRARGLCYTCGSP GHYQAQCPKKRKSGNSRERCQLCDGMGHNAKQCRKRDGNQGQRPGRGLSSGPWPGPEQP AVSLAMTMEHKDRPLVRVILTNTGSHPVKQRSVYITALLDSGADITIISEEDWPTDWPV VDTANPQIHGIGGGIPMRKSRDMIEVGVINRDGSLERPLLLFPAVAMVRGSILGRDCLQ GLGLRLTNL" gene 663..1193 /label=p19 /note="p19" intron 681..682 /label=splice donor /note="splice site (beginning of intron)" gene 1200..1385 /label=p10 /note="p10" gene 1386..2105 /label=p27 /note="p27" gene 2133..2399 /label=p12 /note="p12" gene 2400..2771 /product="protease" /label=p15-protease /note="p15" CDS 2771..5479 /codon_start=1 /label=pol /note="pol" /translation="IGRATVLTVALHLAIPLKWKSDRTPVWIDQWPLPEGKLVALTQLV EKELQLGHIEPSLSCWNTPVFVIRKASGSYRLLHDLRAVNAKLVPFGAVQQGAPVLSAL PRGWPLMVLDLKDCFFSIPLAEQDREAFAFTLPSVNNQAPARRFQWKVLPQGMTCSPTI CQLVVGQVLEPLRLKHPALRMLHYMDDLLLAASSHDGLEAAGKEVIGTLERAGFTISPD KIQREPGVQYLGYKLGSTYVAPVGLVAEPRIATLWDVQKLVGSLQWLRPALGIPPRLMG PFYEQLRGSDPNEAREWNLDMKMAWREIVQLSTTAALERWDPAQPLEGAVARCEQGAIG VLGQGLSTHPRPCLWLFSTQPTKAFTAWLEVLTLLITKLRASAVRTFGKEVDILLLPAC FREDLPLPEGILLALRGFAGKIRSSDTPSIFDIARPLHVSLKVRVTDHPVPGPTVFTDA SSSTHKGVVVWREGPRWEIKEIVDLGASVQQLEARAVAMALLLWPTTPTNVVTDSAFVA KMLLKMGQEGVPSTAAAFILEDALSQRSAMAAVLHVRSHSEVPGFFTEGNDVADSQATF QAYPLREAKDLHTALHIGPRALSKACNISMQQAREVVQTCPHCNSAPALEAGVNPRGLG PLQIWQTDFTLEPRMAPRSWLAVTVDTASSAIVVTQHGRVTSVAAQHHWATAIAVLGRP KAIKTDNGSCFTSRSTREWLARWGIAHTTGIPGNSQGQAMVERANRLLKDKIRVLAEGD GFMKRIPASKQGELLAKAMYALNHFERGENTKTPVQKHWRPTVLTEGPPVKIRIETGEW EKGWNVLVWGRGYAAVKNRDTDKVIWVPSRKVKPDVTQKDEVTKKDEASPLFAGISDWI PWEDEQEGLQGETASNKQERPGEDTLAANES" CDS 2915..3481 /label=reverse transcriptase CDS 4112..4507 /label=RNase H CDS 4664..5155 /label=Integrase intron 5365..5366 /label=splice acceptor /label=splice site /note="splice site (end of intron)" CDS 5367..5534 /codon_start=1 /label=env-sigPE(A) /note="env-sigPE(A)" /translation="AFLTGYPGKTSKKDSKEKPLATSKKDPEKTPLLPTRVNYILIIGV LVLCEVTGVRA" CDS 5535..7151 /codon_start=1 /label=envE /translation="DVHLLEQPGNLWITWANRTGQTDFCLSTQSATSPFQTCLIGIPSP ISEGDFKGYVSDTNCTTLGTDRLVSSASITGGPDNSTTLTYRKVSCLLLKLNVSMWDEP PELQLLGSQSLPNITNITQISGVTGGCVGFAPHSNPSGVYGWGRRQVTHNFLIAPWVNP FFNSASNSTEPFTVVTADRHNLFMGSEYCGAYGYRFWEIYNCSHRFDNFDIYTCGDVQT VKSPEKQCVGGGGIWVNQSKEINETEPFSFTANCTASNLGNVSGCCGKTITILPSGAWV DSTQGSFTKPKALPPAIFLICGDRAWQGIPSRPVGGPCYLGKLTMLAPKHTDILKVLVN SSRTGIRRKRSTSHLDDTCSDEVQLWGPTARIFASILAPGVAAAQALREIERLACWSVK QANLTTSLLGDLLDDVTSIRHAVLQNRAAIDFLLLAHGHGCEDVAGMCCFNLSDHSESI QKKFQLMKEHVNKIGVDSDPIGSWLRGLFGGIGEWAVHLLKGLLLGLVVILLLVVCLPC LLQIVCGNIRKMINNSISYHTE" CDS 7152..7202 /codon_start=1 /label=EnvA /note="gp37" /translation="YKKLQKACGQPESRIV" intron 7303..7304 /label=splice acceptor CDS 7364..8041 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Strongin et al., 2007)" /label=DsRed-Monomer /note="mammalian codon-optimized" /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS GSQ" LTR 8184..8569 /label=dLTR V_segment complement(8570..11313) /label=pLitmus 28i promoter complement(8625..8643) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(8664..8680) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind 8688..8704 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operatortoinhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8712..8742) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8757..8778 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in thepresenceof cAMP." rep_origin complement(8839..9427) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" rep_origin 9538..10047 /direction=RIGHT /label=M13 ori /note="M13 bacteriophage origin ofreplication;arrowindicates direction of (+) strand synthesis" CDS complement(10089..10949) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERSPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(10950..11054) /gene="bla" /label=AmpR promoter primer_bind 11275..11291 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 11295..11313 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" ORIGIN 1 tgtagtctta atcataggtt aacatgtata ttaccaaata agggaatcgc ctgatgcacc 61 aaataaggta ttatatgatc ccattggtgg cgagggagcg acctgagggc atatgggcgt 121 taacagaact gtctgtcctt gcgtcattcc ttcctcatcg gatcatgtac gcggcagagt 181 atgattggat aacaggatgg caccattcat cgtggcgcat gctgattggt gcgactaagg 241 agttatgtaa cccacgaatg tacttaagct tgtagttgct aacaataaag ggccattcta 301 cctctcacca cattggtgtg cacctgggtt gatggccgga ccgtcgattc cctgacgact 361 acgagcacct gaatgaagca gaaggcttca tttggtgacc ccgacgtgat cgttagggaa 421 tagtggtcgg ccacaggcgg cgtggcgatc ctgtctccat ccgtctcgtc gaccgggagg 481 cgagttcgat gaccctgata gagggggctg cggcttaggg aggcagaagc tgagcaccgt 541 cggtaggagc tccagggccc ggagcgactg acccctgccg agaactcaga gggtcgtcgg 601 aagacggaga gtgagcccga cgaccacccc aggcacgtct ttggtcggcc tgcggatcaa 661 gcatggaagc cgtcattaag gtgatttcgt ccgcgtgtaa aacctattgc gggaaaatct 721 ctccttctaa gaaggaaata ggggccatgt tgtccctgtt acaaaaggaa gggttgctta 781 tgtctccctc agatttatat tctccggggt cctgggatcc catcactgcg gcgctctccc 841 agcgggcaat ggtacttgga aaatcgggag agttaaaaac ctggggattg gttttggggg 901 cattgaaggc ggctcgagag gaacaggtta catctgagca agcaaagttt tggttgggat 961 tagggggagg gagggtctct cccccaggtc cggagtgcat cgagaaacca gctacggagc 1021 ggcgaatcga caaaggggag gaggtgggag aaacaactgt gcagcgagat gcgaagatgg 1081 cgccagagga agcggccaca cctaaaaccg ttggcacatc ctgctatcat tgcggaacag 1141 ctgttggctg caattgcgcc accgccacag cctcggcccc tcctccccct tatgtgggga 1201 gtggtttgta tccttccctg gcgggggtgg gagagcagca gggccaggga gataacacgt 1261 ctcggggggc ggagcagcca agggaggagc cagggcacgc gggtcaggcc cctgggccgg 1321 ccctgactga ctgggcaagg gtaagggagg agcttgcgag tactggtccg cccgtggtgg 1381 ccatgcctgt agtgattaag acagagggac ccgcctggac ccctctggag ccaaaattga 1441 tcacaagact ggctgatacg gtcaggacca agggcttacg atccccgatc actatggcag 1501 aagtggaagc gctcatgtcc tccccgttgc tgccgcatga cgtcacgaat ctaatgagag 1561 tgattttagg acctgcccca tatgccttat ggatggacgc ttggggagtc caactccaga 1621 cggttatagc ggcagccact cgcgaccccc gacacccagc gaacggtcaa gggcgggggg 1681 aacggactaa cttggatcga ttaaagggct tagctgatgg gatggtgggc aacccacagg 1741 gtcaggccgc attattaaga ccgggggaat tggttgctat tacggcgtcg gctctccagg 1801 cgtttagaga agttgcccgg ctggcggaac ctgcaggtcc atgggcggac atcacgcagg 1861 gaccatctga gtcctttgtt gattttgcca atcggcttat aaaggcggtt gaggggtcag 1921 atctcccgcc ttccgcgcgg gctccggtga tcattgactg ctttaggcag aagtcacagc 1981 cagatattca gcagcttata cgggcagcac cctccacgct gaccacccca ggagagataa 2041 tcaaatatgt gctagacagg cagaagattg cccctcttac ggatcaaggc atagccgcgg 2101 ccatgtcgtc tgctatccag cccttagtta tggcagtagt caatagagag agggatggac 2161 aaactgggtc gggtggtcgt gcccgagggc tctgctacac ttgtggatcc ccgggacatt 2221 atcaggcaca gtgcccgaaa aaacgaaagt caggaaacag ccgtgagcga tgtcagctgt 2281 gtgacgggat gggacacaac gctaaacagt gtaggaagcg ggatggcaac cagggccaac 2341 gcccaggaag aggtctctct tcggggccgt ggcccggccc tgagcagcct gccgtctcgt 2401 tagcgatgac aatggaacat aaagatcgcc ccttggttag ggtcattctg actaacactg 2461 ggagtcatcc agtcaaacaa cgttcggtgt atatcaccgc gctgttggac tccggagcgg 2521 acatcactat tatttcggag gaggattggc ctactgattg gccggtggtg gacaccgcga 2581 acccacagat ccatggcata ggagggggaa ttcccatgcg aaaatcccgg gatatgatag 2641 aggtgggggt tattaaccga gacgggtcgt tggagcgacc cctgctcctc ttccccgcag 2701 tcgctatggt tagagggagt atcctaggaa gagattgtct gcagggccta gggctccgct 2761 tgacaaattt atagggaggg ccactgttct tactgttgcg ctacatctgg ctattccgct 2821 caaatggaag tcagaccgca cgcctgtgtg gattgaccag tggccccttc ctgaaggtaa 2881 acttgtagcg ctaacgcaat tagtggaaaa agaattacag ttaggacata tagagccctc 2941 acttagttgt tggaacacac ctgtttttgt gatccggaag gcttccgggt cttatcgctt 3001 attgcatgat ttgcgcgctg ttaacgccaa gcttgtccct tttggggccg tccaacaggg 3061 ggcgccagtt ctctccgcgc tcccgcgtgg ctggcccctg atggtcctag acctcaagga 3121 ttgcttcttt tctatccctc ttgcggaaca agatcgcgaa gcttttgcat ttacgctccc 3181 ctctgtgaat aaccaggccc ccgctcgaag attccaatgg aaggtcttgc cccaagggat 3241 gacctgttct cccactatct gtcagttggt agtgggtcag gtgctcgagc ccttgcgact 3301 caagcaccca gctctgcgca tgttgcatta tatggacgat cttttgctag ccgcctcaag 3361 tcatgatggg ttggaagcgg cagggaagga ggttatcggt acattggaaa gagccgggtt 3421 cactatttcg ccggataaga tccagaggga gcccggagta caatatcttg ggtacaagtt 3481 aggcagtacg tatgtagcac ccgtaggctt ggtagcagaa cccaggatag ccaccttgtg 3541 ggatgttcaa aagctggtgg ggtcacttca gtggcttcgc ccagcgttag ggatcccgcc 3601 acgactgatg ggtccctttt atgagcagtt acgagggtca gatcctaacg aggcgaggga 3661 atggaatcta gacatgaaaa tggcctggag agagatcgta cagcttagca ctactgctgc 3721 cttggaacga tgggaccctg cccagcctct ggaaggagcg gtcgctagat gtgaacaggg 3781 ggcaataggg gtcctgggac agggactgtc cacacaccca aggccatgtt tgtggttatt 3841 ctccacccaa cccaccaagg cgtttactgc ttggttagaa gtgctcaccc ttttgattac 3901 taagctacgc gcttcggcag tgcgaacctt tggcaaggag gttgatatcc tcctgttgcc 3961 tgcatgcttc cgggaggacc ttccgctccc ggaggggatc ctgttagcac ttagggggtt 4021 tgcaggaaaa atcaggagta gtgacacgcc atctattttt gacattgcgc gtccactgca 4081 tgtttctctg aaagtgaggg ttaccgacca ccctgtgccg ggacccactg tctttaccga 4141 cgcctcctca agcacccata aaggggtggt agtctggagg gagggcccaa ggtgggagat 4201 aaaagaaata gttgatttgg gggcaagtgt acaacaactg gaggcacgcg ctgtggccat 4261 ggcacttctg ctgtggccga caacgcccac taatgtagtg actgactctg cgtttgttgc 4321 gaaaatgtta ctcaagatgg gacaggaggg agtcccgtct acagcggcag cttttatttt 4381 agaggatgcg ttaagccaaa ggtcagccat ggccgccgtt ctccacgtgc ggagtcattc 4441 tgaagtgcca gggtttttca cagaaggaaa tgacgtggca gatagccaag ccacctttca 4501 agcgtatccc ttgagagagg ctaaagatct tcataccgct ctccatattg gaccccgcgc 4561 gctatccaaa gcgtgtaata tatctatgca gcaggctagg gaggttgttc agacctgccc 4621 gcattgtaat tcagcccctg cgttggaggc cggggtaaac cctaggggtt tgggacccct 4681 acagatatgg cagacagact ttacgcttga gcctagaatg gctccccgtt cctggctcgc 4741 tgttactgtg gacaccgcct catcagcgat agtcgtaact cagcatggcc gtgttacatc 4801 ggttgctgca caacatcatt gggccacggc tatcgccgtt ttgggaagac caaaggccat 4861 aaaaacagat aacgggtcct gcttcacgtc tagatccacg cgggagtggc tcgcgagatg 4921 ggggatagca cacaccaccg ggattccggg aaattcccag ggtcaagcta tggtagagcg 4981 ggccaaccgg ctcctgaaag ataagatccg tgtgcttgcg gagggggacg gctttatgaa 5041 aagaatcccc gccagcaaac agggggaact actagccaag gcaatgtatg ccctcaatca 5101 ctttgagcgt ggtgaaaaca caaaaacacc ggtacaaaaa cactggagac ctaccgttct 5161 tacagaagga cccccggtta aaatacgaat agagacaggg gagtgggaaa aaggatggaa 5221 cgtgctggtc tggggacgag gttatgccgc tgtgaaaaac agggacactg ataaggttat 5281 ttgggtaccc tctcggaaag ttaaaccgga tgtcacccaa aaggatgagg tgactaagaa 5341 agatgaggcg agccctcttt ttgcaggcat ttctgactgg ataccctggg aagacgagca 5401 agaaggactc caaggagaaa ccgctagcaa caagcaagaa agacccggag aagacaccct 5461 tgctgccaac gagagttaat tatattctca ttattggtgt cctggtcttg tgtgaggtta 5521 cgggggtaag agctgatgtc cacttactcg agcagccagg gaacctttgg attacatggg 5581 ccaaccgtac aggccaaacg gatttctgcc tctctacaca gtcagccacc tccccttttc 5641 aaacatgttt gataggtatc ccgtccccta tttccgaagg tgattttaag ggatatgtct 5701 ctgatacaaa ttgcaccacc ttgggaactg accggttagt ctcgtcagcc agcattaccg 5761 gcggccctga caacagcacc accctcactt atcgaaaggt ttcatgcttg ctgttaaagc 5821 tgaatgtctc tatgtgggat gagccaccgg aactacagct gctaggttcc cagtctctcc 5881 ctaacattac taatattact cagatttctg gtgtaaccgg gggatgcgta ggcttcgccc 5941 cacactccaa tccaagtggt gtctacgggt ggggccggag acaggttaca cacaacttct 6001 tgatcgcccc gtgggtcaat cctttcttta acagcgcttc taactccacg gaaccgttta 6061 cggtggtgac agcggatagg cacaatcttt ttatggggag tgagtactgc ggtgcatatg 6121 gctacagatt ttgggaaata tataattgct cacacagatt tgataatttt gatatttaca 6181 cctgtggaga tgtgcagaca gtcaaatccc ccgaaaaaca gtgtgtgggg ggaggaggta 6241 tatgggttaa tcaatcaaag gaaattaatg agacagagcc gttcagtttt actgcgaact 6301 gtacagctag taatttgggt aatgtcagcg gatgttgtgg aaaaacgatc acgattctcc 6361 catcaggggc gtgggtcgac agcacacaag gtagtttcac caaaccaaaa gcgctaccac 6421 ccgcaatttt cctcatttgt ggggatcgcg catggcaagg aattcccagt cgtccggtag 6481 ggggcccctg ctatttaggc aagcttacca tgttagcacc taagcataca gatattctca 6541 aggtgcttgt caattcatcg cggacaggta taagacgtaa acgaagcacc tcacacctgg 6601 atgatacatg ctcagatgaa gtgcagcttt ggggtcctac agcaagaatc tttgcatcta 6661 tcctagcccc gggggtagca gctgcgcaag ccttaagaga aattgagaga ctagcctgtt 6721 ggtccgttaa acaggctaac ttgacaacat cactcctcgg ggacttattg gatgatgtca 6781 cgagtattcg acacgcggtc ctgcagaacc gagcggctat tgacttcttg ctcctagctc 6841 acggccatgg ctgtgaggac gttgccggaa tgtgctgttt caatttgagt gatcacagtg 6901 agtctataca gaagaagttc cagctaatga aggaacatgt caataagatc ggcgtggaca 6961 gcgacccaat tggaagttgg ctgcgaggac tattcggggg aataggagaa tgggccgttc 7021 atttgctgaa aggactgctt ttggggcttg tagttatttt gttgctagta gtgtgcctgc 7081 cttgcctttt gcaaatcgta tgcggtaaca tcagaaagat gattaataac tccatcagct 7141 accacacgga atataagaag ctgcaaaagg cctgtgggca gcctgaaagc agaatagtat 7201 aaggcagtac atgggtggtg gtatagcgct tgcgagtcca tcgaggaagg caggaaagac 7261 agctattggt aattgtgaaa tacgcttttg tctgtgtgct gcaggagctg agctgactct 7321 gctggtggcc tcgcgtacca ctgtggcatc gatggtcgcc accatggaca acaccgagga 7381 cgtcatcaag gagttcatgc agttcaaggt gcgcatggag ggctccgtga acggccacta 7441 cttcgagatc gagggcgagg gcgagggcaa gccctacgag ggcacccaga ccgccaagct 7501 gcaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcca 7561 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactaca tgaagctgtc 7621 cttccccgag ggcttcacct gggagcgctc catgaacttc gaggacggcg gcgtggtgga 7681 ggtgcagcag gactcctccc tgcaggacgg caccttcatc tacaaggtga agttcaaggg 7741 cgtgaacttc cccgccgacg gccccgtaat gcagaagaag actgccggct gggagccctc 7801 caccgagaag ctgtaccccc aggacggcgt gctgaagggc gagatctccc acgccctgaa 7861 gctgaaggac ggcggccact acacctgcga cttcaagacc gtgtacaagg ccaagaagcc 7921 cgtgcagctg cccggcaacc actacgtgga ctccaagctg gacatcacca accacaacga 7981 ggactacacc gtggtggagc agtacgagca cgccgaggcc cgccactccg gctcccagta 8041 gagatcgatg cgatgtacgg gccagatata cgcgtatctg aggggactag ggtgtgttta 8101 ggcgaaaagc ggggcttcgg ttgtacgcgg ttaggagtcc cctcgaggta tggcagatat 8161 gcttttgcat agggaggggg aaatgtagtc ttaatcatag gttaacatgt atattaccaa 8221 ataagggaat cgcctgatgc accaaataag gtattatatg atcccattgg tggcgaggga 8281 gcgacctgag ggcatatggg cgttaacaga actgtctgtc cttgcgtcat tccttcctca 8341 tcggatcatg tacgcggcag agtatgattg gataacagga tggcaccatt catcgtggcg 8401 catgctgatt ggtgcgacta aggagttatg taacccacga atgtacttaa gcttgtagtt 8461 gctaacaata aagggccatt ctacctctca ccacattggt gtgcacctgg gttgatggcc 8521 ggaccgtcga ttccctgacg actacgagca cctgaatgaa gcagaaggca tcctgcagga 8581 attcctcgag acgtacggtg cgcgcgatgc attcgaagat ctgccctata gtgagtcgta 8641 ttacgtagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 8701 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 8761 gtgagctaac tcacattaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 8821 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 8881 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 8941 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 9001 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 9061 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 9121 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 9181 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 9241 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc 9301 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 9361 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 9421 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 9481 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttacg 9541 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta 9601 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt 9661 tcgctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt 9721 acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc 9781 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt 9841 gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat 9901 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa 9961 ttttaacaaa atattaacgt ttacaattta aatatttgct tatacaatct tcctgttttt 10021 ggggcttttc tgattatcaa ccggggtaaa tcaatctaaa gtatatatga gtaaacttgg 10081 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 10141 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 10201 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 10261 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 10321 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 10381 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 10441 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 10501 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 10561 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 10621 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 10681 ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta 10741 aaagtgctca tcattggaga acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 10801 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 10861 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 10921 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 10981 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 11041 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtagttaa caaaaaaaag 11101 cccgccgaag cgggctttat taccaagcga agcgccattc gccattcagg ctgcgcaact 11161 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat 11221 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa 11281 cgacggccag tccgtaatac gactcactat agg //