{"text_format":"3","workbook":"xls","n_sheets":7,"sheets":["Gene Table","Average Ct","Average Delta(Ct)","2^(-Avg.(Delta(Ct))","Fold Change","p-value","Fold Regulation"],"not_rendered":{"external_links":1,"defined_names":1},"read":"whole","date_system":"1900","formulas":"cached_only"}

{"sheet":"Gene Table","index":0,"n_rows":99,"n_cols":7,"rows_kept":99,"merged":[[0,0,0,1]],"flags":["formulas_cached_only"]}
["PCR Array Catalog #:",null,"PARN-043Z"]
[]
["Position","Unigene","Refseq","Symbol","Description","Gname","RT2 Catalog"]
["A01","Rn.88057","NM_012499","Apc","Adenomatous polyposis coli","RATAPC","PPR52699A"]
["A02","Rn.31781","NM_024405","Axin1","Axin 1","Axin","PPR48454B"]
["A03","Rn.162212","NM_024355","Axin2","Axin 2","axil","PPR45934A"]
["A04","N/A","NM_001107703","Bcl9","B-cell CLL/lymphoma 9","-","PPR47136A"]
["A05","Rn.21800","NM_001007148","Btrc","Beta-transducin repeat containing","beta-TrCP1","PPR47206F"]
["A06","Rn.198958","NM_001105969","Vangl2","Vang-like 2 (van gogh, Drosophila)","Ltap","PPR54102B"]
["A07","Rn.229691","NM_145676","Cby1","Chibby homolog 1 (Drosophila)","Pgea1","PPR42946A"]
["A08","Rn.22279","NM_171992","Ccnd1","Cyclin D1","-","PPR06517C"]
["A09","Rn.96083","NM_022267","Ccnd2","Cyclin D2","-","PPR06521A"]
["A10","Rn.23810","NM_053615","Csnk1a1","Casein kinase 1, alpha 1","-","PPR47697A"]
["A11","Rn.4231","NM_053824","Csnk2a1","Casein kinase 2, alpha 1 polypeptide","-","PPR43385G"]
["A12","Rn.112601","NM_053357","Ctnnb1","Catenin (cadherin associated protein), beta 1","Catnb","PPR57457A"]
["B01","Rn.92012","NM_001108030","Daam1","Dishevelled associated activator of morphogenesis 1","-","PPR52991B"]
["B02","Rn.161784","NM_024159","Dab2","Disabled homolog 2 (Drosophila)","Doc-2/Doc2","PPR45982B"]
["B03","Rn.8331","NM_001037654","Dixdc1","DIX domain containing 1","Ccd1","PPR54525A"]
["B04","Rn.214343","NM_001106350","Dkk1","Dickkopf homolog 1 (Xenopus laevis)","-","PPR59973A"]
["B05","Rn.12516","NM_138519","Dkk3","Dickkopf homolog 3 (Xenopus laevis)","Reic/p29","PPR45645F"]
["B06","Rn.163335","NM_001109332","Dkk4","Dickkopf homolog 4 (Xenopus laevis)","RGD1563172","PPR49830C"]
["B07","Rn.144610","NM_031820","Dvl1","Dishevelled, dsh homolog 1 (Drosophila)","dvl-1","PPR52969A"]
["B08","Rn.153569","NM_001172056","Dvl2","Dishevelled 2","Dvl-2","PPR47476E"]
["B09","Rn.12447","XM_001076610","Ep300","E1A binding protein p300","-","PPR50202A"]
["B10","Rn.159760","NM_001106993","Fbxw11","F-box and WD repeat domain containing 11","-","PPR45806A"]
["B11","Rn.81223","NM_053809","Fgf4","Fibroblast growth factor 4","Hst/Hst1","PPR52297E"]
["B12","Rn.11306","NM_012953","Fosl1","Fos-like antigen 1","Fra-1/fra-1","PPR45356A"]
["C01","Rn.12034","NM_001100527","Frzb","Frizzled-related protein","-","PPR45566A"]
["C02","Rn.6575","NM_021266","Fzd1","Frizzled homolog 1 (Drosophila)","-","PPR57723A"]
["C03","Rn.92324","NM_172035","Fzd2","Frizzled homolog 2 (Drosophila)","Fz-10/Fzd10","PPR53045B"]
["C04","Rn.178695","NM_153474","Fzd3","Frizzled homolog 3 (Drosophila)","-","PPR46597F"]
["C05","Rn.48736","NM_022623","Fzd4","Frizzled homolog 4 (Drosophila)","-","PPR50270B"]
["C06","Rn.24792","NM_173838","Fzd5","Frizzled homolog 5 (Drosophila)","-","PPR47915A"]
["C07","Rn.2740","NM_001130536","Fzd6","Frizzled homolog 6 (Drosophila)","-","PPR53095A"]
["C08","N/A","XM_006226838","Fzd7","Frizzled family receptor 7","-","PPR59971C"]
["C09","Rn.96335","NM_153305","Fzd9","Frizzled homolog 9 (Drosophila)","-","PPR53449A"]
["C10","Rn.36807","NM_017344","Gsk3a","Glycogen synthase kinase 3 alpha","-","PPR48938E"]
["C11","Rn.10426","NM_032080","Gsk3b","Glycogen synthase kinase 3 beta","-","PPR44848A"]
["C12","Rn.93714","NM_021835","Jun","Jun oncogene","-","PPR53221A"]
["D01","Rn.90021","NM_053649","Kremen1","Kringle containing transmembrane protein 1","Kremen","PPR52798C"]
["D02","Rn.218464","NM_001105767","Kremen2","Kringle containing transmembrane protein 2","-","PPR59974B"]
["D03","Rn.228611","NM_130429","Lef1","Lymphoid enhancer binding factor 1","-","PPR47223A"]
["D04","Rn.12698","NM_001106321","Lrp5","Low density lipoprotein receptor-related protein 5","-","PPR45688A"]
["D05","Rn.229041","NM_001107892","Lrp6","Low density lipoprotein receptor-related protein 6","-","PPR48581B"]
["D06","Rn.4090","NM_053829","Mapk8","Mitogen-activated protein kinase 8","JNK","PPR43333A"]
["D07","Rn.31427","NM_001191089","Mitf","Microphthalmia-associated transcription factor","-","PPR52009A"]
["D08","Rn.10282","NM_012864","Mmp7","Matrix metallopeptidase 7","MPMM","PPR44767C"]
["D09","Rn.12072","NM_012603","Myc","Myelocytomatosis oncogene","RNCMYC/c-myc/mMyc","PPR45580A"]
["D10","Rn.8826","NM_001108894","Nkd1","Naked cuticle homolog 1 (Drosophila)","-","PPR47956A"]
["D11","Rn.198099","NM_001107454","Nkd2","Naked cuticle homolog 2 (Drosophila)","-","PPR54553A"]
["D12","Rn.17591","NM_019334","Pitx2","Paired-like homeodomain 2","Pitx-2/Pitx2c/Ptx2","PPR46507F"]
["E01","Rn.63525","NM_001173355","Porcn","Porcupine homolog (Drosophila)","RGD1564947","PPR51406A"]
["E02","Rn.96181","NM_013141","Ppard","Peroxisome proliferator-activated receptor delta","Pparb","PPR53429A"]
["E03","Rn.141040","NM_199396","Prickle1","Prickle homolog 1 (Drosophila)","Rilp","PPR52623A"]
["E04","Rn.24988","NM_001106447","Pygo2","Pygopus 2","RGD1308535","PPR55674A"]
["E05","Rn.29157","NM_134366","Rac1","Ras-related C3 botulinum toxin substrate 1","-","PPR48291A"]
["E06","N/A","XM_344122","RGD1561440","Similar to nemo like kinase","-","PPR47865A"]
["E07","Rn.107401","NM_057132","Rhoa","Ras homolog gene family, member A","Arha/Arha2","PPR56555A"]
["E08","Rn.163333","NM_001276712","Sfrp1","Secreted frizzled-related protein 1","sFRP-1","PPR54165F"]
["E09","Rn.102416","NM_001100700","Sfrp2","Secreted frizzled-related protein 2","-","PPR54888A"]
["E10","Rn.10788","NM_053544","Sfrp4","Secreted frizzled-related protein 4","-","PPR45047B"]
["E11","Rn.40184","NM_001107591","Sfrp5","Secreted frizzled-related protein 5","-","PPR49236A"]
["E12","Rn.33103","NM_001107865","Tcf7l1","Transcription factor 3","Tcf3","PPR48590B"]
["F01","Rn.23354","NM_053369","Tcf4","Transcription factor 4","-","PPR47578B"]
["F02","Rn.106335","XM_006220666","Tcf7","Transcription factor 7 (T-cell specific, HMG-box)","-","PPR56274B"]
["F03","Rn.10290","NM_133524","Tcf3","Transcription factor E2a","Pan2/Tcfe2a","PPR44771A"]
["F04","Rn.6875","NM_001173433","Tle1","Transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila)","-","PPR43716A"]
["F05","Rn.74256","NM_053738","Wif1","Wnt inhibitory factor 1","-","PPR51990A"]
["F06","Rn.63486","NM_031716","Wisp1","WNT1 inducible signaling pathway protein 1","-","PPR51404A"]
["F07","Rn.138108","NM_001105714","Wnt1","Wingless-type MMTV integration site family, member 1","Int1","PPR57724A"]
["F08","Rn.18155","NM_001108227","Wnt10a","Wingless-type MMTV integration site family, member 10A","-","PPR46593A"]
["F09","Rn.218544","NM_001108111","Wnt10b","Wingless-type MMTV integration site family, member 10B","-","PPR59979A"]
["F10","Rn.222290","NM_080401","Wnt11","Wingless-type MMTV integration site family, member 11","-","PPR57726F"]
["F11","Rn.223092","XM_575397","Wnt2","Wingless-type MMTV integration site family member 2","Wnt","PPR57593A"]
["F12","Rn.17219","NM_001191848","Wnt2b","Wingless-type MMTV integration site family, member 2B","-","PPR46440A"]
["G01","Rn.148140","NM_001105715","Wnt3","Wingless-type MMTV integration site family, member 3","INT4","PPR57725A"]
["G02","Rn.234269","NM_001107005","Wnt3a","Wingless-type MMTV integration site family, member 3A","-","PPR59975A"]
["G03","Rn.34782","NM_053402","Wnt4","Wingless-type MMTV integration site family, member 4","-","PPR48754A"]
["G04","Rn.48749","NM_022631","Wnt5a","Wingless-type MMTV integration site family, member 5A","-","PPR50278A"]
["G05","Rn.58724","NM_001100489","Wnt5b","Wingless-type MMTV integration site family, member 5B","-","PPR53096A"]
["G06","Rn.22149","NM_001108226","Wnt6","Wingless-type MMTV integration site family, member 6","-","PPR47266A"]
["G07","Rn.230035","NM_001100473","Wnt7a","Wingless-type MMTV integration site family, member 7A","-","PPR56658B"]
["G08","Rn.129727","NM_001009695","Wnt7b","Wingless-type MMTV integration site family, member 7B","-","PPR59943E"]
["G09","Rn.218696","NM_001106155","Wnt8a","Wingless-type MMTV integration site family, member 8A","-","PPR59978A"]
["G10","Rn.58872","NM_001106359","Wnt8b","Wingless-type MMTV integration site family, member 8B","-","PPR51094A"]
["G11","Rn.40411","NM_001105783","Wnt9a","Wingless-type MMTV integration site family, member 9A","-","PPR55586B"]
["G12","Rn.16625","NM_001107055","Wnt9b","Wingless-type MMTV integration site family, member 9B","-","PPR59977A"]
["H01","Rn.94978","NM_031144","Actb","Actin, beta","Actx","PPR06570C"]
["H02","Rn.1868","NM_012512","B2m","Beta-2 microglobulin","-","PPR42607A"]
["H03","Rn.47","NM_012583","Hprt1","Hypoxanthine phosphoribosyltransferase 1","Hgprtase/Hprt","PPR42247F"]
["H04","Rn.107896","NM_017025","Ldha","Lactate dehydrogenase A","Ldh1","PPR56603B"]
["H05","Rn.973","NM_001007604","Rplp1","Ribosomal protein, large, P1","-","PPR42363C"]
["H06","N/A","U26919","RGDC","Rat Genomic DNA Contamination","RGDC"]
["H07","N/A","SA_00104","RTC","Reverse Transcription Control","RTC","PPX63340A"]
["H08","N/A","SA_00104","RTC","Reverse Transcription Control","RTC","PPX63340A"]
["H09","N/A","SA_00104","RTC","Reverse Transcription Control","RTC","PPX63340A"]
["H10","N/A","SA_00103","PPC","Positive PCR Control","PPC"]
["H11","N/A","SA_00103","PPC","Positive PCR Control","PPC"]
["H12","N/A","SA_00103","PPC","Positive PCR Control","PPC"]

{"sheet":"Average Ct","index":1,"n_rows":98,"n_cols":6,"rows_kept":98,"merged":[[0,0,1,0],[0,1,1,1],[0,2,0,3],[0,4,0,5]],"flags":["formulas_cached_only"]}
["Position","Symbol","AVG Ct",null,"Standard Deviation"]
[null,null,"Control Group","Group 1","Control Group","Group 1"]
["A01","Apc",26.69,27.28,0.205264,0.868639]
["A02","Axin1",26.63,27.38,0.190875,0.457857]
["A03","Axin2",26.2,26.73,0.245832,0.869655]
["A04","Bcl9",28.34,28.55,0.37581,0.899129]
["A05","Btrc",26.79,27.26,0.305123,0.718076]
["A06","Vangl2",34.75,35,0.427239,0]
["A07","Cby1",26.89,26.88,0.481802,0.629471]
["A08","Ccnd1",23.21,23.38,0.295014,0.335609]
["A09","Ccnd2",26.51,26.32,0.07,0.626418]
["A10","Csnk1a1",23.54,24.16,0.302379,0.750555]
["A11","Csnk2a1",24.89,25.31,0.112694,0.580259]
["A12","Ctnnb1",23.4,23.45,0.355387,0.590282]
["B01","Daam1",24.11,27.1,2.275837,0.094516]
["B02","Dab2",24.29,25.01,0.20502,0.582437]
["B03","Dixdc1",27.46,27.52,0.252389,0.871952]
["B04","Dkk1",29.38,29.71,0.225019,0.77106]
["B05","Dkk3",21.26,21.91,0.06245,0.681982]
["B06","Dkk4",31.37,32.46,0.765005,1.20093]
["B07","Dvl1",25.96,26.18,0.298719,0.696372]
["B08","Dvl2",30.26,30.61,0.390043,0.859089]
["B09","Ep300",25.76,26.28,0.155027,1.049397]
["B10","Fbxw11",25.38,25.75,0.29023,0.73]
["B11","Fgf4",35,35,0,0]
["B12","Fosl1",25.27,25.96,0.228983,0.8778]
["C01","Frzb",30.65,30.31,0.18735,0.549909]
["C02","Fzd1",24.12,24.73,0.123423,0.933113]
["C03","Fzd2",27.07,27.14,0.160416,0.747953]
["C04","Fzd3",30.22,30.56,0.115902,1.205667]
["C05","Fzd4",27,27.36,0.155027,0.727942]
["C06","Fzd5",26.74,29.84,2.019629,0.827124]
["C07","Fzd6",27.27,27.7,0.060277,0.746079]
["C08","Fzd7",33.48,33.86,0.295691,0.915114]
["C09","Fzd9",34.11,35,0.35,0]
["C10","Gsk3a",24.43,24.84,0.225019,0.678847]
["C11","Gsk3b",24.82,25.04,0.032146,1.06491]
["C12","Jun",25.32,25.21,0.210079,0.568243]
["D01","Kremen1",28.13,28.29,0.272213,0.701712]
["D02","Kremen2",34.44,34.6,0.348473,0.427824]
["D03","Lef1",32.92,33.28,1.517575,1.541309]
["D04","Lrp5",24.99,25.35,0.16773,0.488774]
["D05","Lrp6",23.16,23.84,0.141774,1.126469]
["D06","Mapk8",27.83,28.7,0.353601,0.498029]
["D07","Mitf",25.02,25.19,0.11,1.022562]
["D08","Mmp7",35,35,0,0]
["D09","Myc",24.52,24.9,0.072342,0.597746]
["D10","Nkd1",27.69,28.28,0.265581,0.635531]
["D11","Nkd2",25.15,26.66,0.080829,0.789325]
["D12","Pitx2",32.66,34.43,2.045002,0.59702]
["E01","Porcn",26.22,26.47,0.643765,0.652559]
["E02","Ppard",26,26.41,0.16,0.720162]
["E03","Prickle1",25.52,26.09,0.080829,0.757826]
["E04","Pygo2",26.6,27.81,0.443772,1.426055]
["E05","Rac1",22.62,23.41,0.075719,1.044174]
["E06","RGD1561440",28.67,30.32,0.756395,1.014364]
["E07","Rhoa",20.98,21.34,0.419325,0.42004]
["E08","Sfrp1",34.25,35,0.654319,0]
["E09","Sfrp2",27.95,28.89,0.148436,1.073918]
["E10","Sfrp4",27.28,27.29,0.10116,1.002314]
["E11","Sfrp5",31.89,32.93,0.035119,1.056141]
["E12","Tcf7l1",27.59,27.66,0.18735,0.571781]
["F01","Tcf4",25.07,25.22,0.175784,0.368646]
["F02","Tcf7",31.09,31.58,0.36756,0.622923]
["F03","Tcf3",26.42,27.11,0.375278,0.625167]
["F04","Tle1",26.22,26.8,0.188237,0.621316]
["F05","Wif1",23.17,27.68,4.448045,1.74356]
["F06","Wisp1",25.61,25.89,0.077675,0.964797]
["F07","Wnt1",34.9,35,0.173205,0]
["F08","Wnt10a",33.15,35,1.722566,0]
["F09","Wnt10b",32.15,32.73,0.800021,1.645084]
["F10","Wnt11",31.96,24.57,5.32078,14.352959]
["F11","Wnt2",33.45,35,1.395182,0]
["F12","Wnt2b",31.25,32.41,0.504777,0.719328]
["G01","Wnt3",35,35,0,0]
["G02","Wnt3a",34.91,34.68,0.161658,0.554256]
["G03","Wnt4",28.74,29.18,0.275379,0.902792]
["G04","Wnt5a",28.69,28.98,0.066583,0.818535]
["G05","Wnt5b",28.56,28.08,0.130128,0.595343]
["G06","Wnt6",34.88,29.41,2.07846,4.854527]
["G07","Wnt7a",35,34.74,0,0.44456]
["G08","Wnt7b",30.55,31.7,0.868581,0.232594]
["G09","Wnt8a",33.4,35,1.385785,0]
["G10","Wnt8b",35,35,0,0]
["G11","Wnt9a",28.62,29.68,0.160935,0.386825]
["G12","Wnt9b",33.84,34.95,1.07677,0.080829]
["H01","Actb",17.59,18.06,0.208806,1.039375]
["H02","B2m",21.24,21.84,0.066583,0.669054]
["H03","Hprt1",23.79,24.05,0.011547,0.84002]
["H04","Ldha",19.88,20.63,0.134536,0.519262]
["H05","Rplp1",19.74,20.41,0.341223,0.389744]
["H06","RGDC",35,35,0,0]
["H07","RTC",23.8,24.15,0.192959,0.045826]
["H08","RTC",23.73,23.93,0.090738,0.20306]
["H09","RTC",23.76,23.95,0.110604,0.015275]
["H10","PPC",20.75,21.19,0.435239,0.158745]
["H11","PPC",20.79,21.13,0.315647,0.240208]
["H12","PPC",20.71,21.18,0.453909,0.228546]

{"sheet":"Average Delta(Ct)","index":2,"n_rows":98,"n_cols":6,"rows_kept":98,"merged":[[0,0,1,0],[0,1,1,1],[0,2,0,3],[0,4,0,5]],"flags":["formulas_cached_only"]}
["Position","Symbol","AVG Delta(Ct)  (Ct(GOI) - Ave Ct(HKG))",null,"Standard Deviation"]
[null,null,"Control Group","Group 1","Control Group","Group 1"]
["A01","Apc",6.246,6.279333,0.066363,0.197477]
["A02","Axin1",6.186,6.379333,0.047159,0.333672]
["A03","Axin2",5.749333,5.732667,0.108891,0.204101]
["A04","Bcl9",7.889333,7.556,0.236257,0.235635]
["A05","Btrc",6.342667,6.259333,0.161113,0.087506]
["A06","Vangl2",14.306,14.002667,0.397397,0.671265]
["A07","Cby1",6.439333,5.886,0.337842,0.110109]
["A08","Ccnd1",2.766,2.379333,0.150877,0.336597]
["A09","Ccnd2",6.062667,5.322667,0.079607,0.154523]
["A10","Csnk1a1",3.089333,3.166,0.158421,0.242619]
["A11","Csnk2a1",4.442667,4.312667,0.03402,0.099485]
["A12","Ctnnb1",2.952667,2.449333,0.215493,0.081347]
["B01","Daam1",3.666,6.106,2.132718,0.577429]
["B02","Dab2",3.846,4.016,0.067557,0.093402]
["B03","Dixdc1",7.012667,6.522667,0.117462,0.212926]
["B04","Dkk1",8.929333,8.716,0.196003,0.1951]
["B05","Dkk3",0.812667,0.912667,0.203316,0.08377]
["B06","Dkk4",10.919333,11.466,0.622589,0.607457]
["B07","Dvl1",5.516,5.186,0.172,0.028]
["B08","Dvl2",9.809333,9.609333,0.526134,0.203021]
["B09","Ep300",5.316,5.286,0.011136,0.551112]
["B10","Fbxw11",4.929333,4.752667,0.146347,0.058799]
["B11","Fgf4",14.552667,14.002667,0.144143,0.671265]
["B12","Fosl1",4.826,4.959333,0.110109,0.336151]
["C01","Frzb",10.202667,9.312667,0.331115,0.264494]
["C02","Fzd1",3.676,3.732667,0.028,0.263016]
["C03","Fzd2",6.619333,6.139333,0.069695,0.109441]
["C04","Fzd3",9.776,9.559333,0.25831,0.534544]
["C05","Fzd4",6.556,6.362667,0.281645,0.091528]
["C06","Fzd5",6.292667,8.846,1.876325,0.155897]
["C07","Fzd6",6.826,6.706,0.109014,0.431421]
["C08","Fzd7",13.029333,12.859333,0.212503,0.247017]
["C09","Fzd9",13.662667,14.002667,0.408261,0.671265]
["C10","Gsk3a",3.986,3.846,0.097386,0.06296]
["C11","Gsk3b",4.376,4.046,0.154415,0.401303]
["C12","Jun",4.869333,4.212667,0.12813,0.117717]
["D01","Kremen1",7.682667,7.292667,0.12813,0.046014]
["D02","Kremen2",13.996,13.606,0.27023,0.552851]
["D03","Lef1",12.469333,12.279333,1.373535,0.873119]
["D04","Lrp5",4.539333,4.352667,0.039716,0.18745]
["D05","Lrp6",2.712667,2.839333,0.05116,0.466773]
["D06","Mapk8",7.379333,7.706,0.209469,0.178]
["D07","Mitf",4.572667,4.189333,0.249715,0.351422]
["D08","Mmp7",14.552667,14.002667,0.144143,0.671265]
["D09","Myc",4.069333,3.902667,0.072231,0.232244]
["D10","Nkd1",7.246,7.282667,0.124756,0.109258]
["D11","Nkd2",4.706,5.659333,0.136836,0.182256]
["D12","Pitx2",12.216,13.436,1.907366,0.572786]
["E01","Porcn",5.769333,5.469333,0.501814,0.364002]
["E02","Ppard",5.552667,5.409333,0.059138,0.093474]
["E03","Prickle1",5.069333,5.092667,0.084246,0.131367]
["E04","Pygo2",6.149333,6.816,0.317643,0.818293]
["E05","Rac1",2.176,2.412667,0.06844,0.372958]
["E06","RGD1561440",8.219333,9.326,0.613822,0.394137]
["E07","Rhoa",0.529333,0.339333,0.276002,0.281811]
["E08","Sfrp1",13.799333,14.002667,0.514721,0.671265]
["E09","Sfrp2",7.506,7.892667,0.19961,0.609145]
["E10","Sfrp4",6.829333,6.296,0.147028,0.406945]
["E11","Sfrp5",11.439333,11.936,0.150524,0.961293]
["E12","Tcf7l1",7.142667,6.666,0.046014,0.099519]
["F01","Tcf4",4.622667,4.222667,0.059475,0.313013]
["F02","Tcf7",10.642667,10.586,0.256198,0.381529]
["F03","Tcf3",5.969333,6.109333,0.233618,0.122871]
["F04","Tle1",5.776,5.806,0.057654,0.053703]
["F05","Wif1",2.722667,6.682667,4.309714,2.798522]
["F06","Wisp1",5.159333,4.889333,0.217663,0.302154]
["F07","Wnt1",14.452667,14.002667,0.179937,0.671265]
["F08","Wnt10a",12.706,14.002667,1.599614,0.671265]
["F09","Wnt10b",11.699333,11.732667,0.893464,1.001747]
["F10","Wnt11",11.512667,3.576,7.13769,13.857587]
["F11","Wnt2",12.999333,14.002667,1.252237,0.671265]
["F12","Wnt2b",10.802667,11.409333,0.387527,0.556181]
["G01","Wnt3",14.552667,14.002667,0.144143,0.671265]
["G02","Wnt3a",14.459333,13.682667,0.172445,1.209577]
["G03","Wnt4",8.289333,8.179333,0.298626,0.237439]
["G04","Wnt5a",8.246,7.982667,0.135514,0.147639]
["G05","Wnt5b",8.109333,7.086,0.184112,0.32293]
["G06","Wnt6",14.432667,8.409333,2.04688,4.988224]
["G07","Wnt7a",14.552667,13.746,0.144143,0.728124]
["G08","Wnt7b",10.099333,10.702667,0.731367,0.515342]
["G09","Wnt8a",12.952667,14.002667,1.243036,0.671265]
["G10","Wnt8b",14.552667,14.002667,0.144143,0.671265]
["G11","Wnt9a",8.172667,8.679333,0.044242,0.321772]
["G12","Wnt9b",13.396,13.956,1.127042,0.748321]
["H01","Actb",-2.857333,-2.937333,0.065248,0.378863]
["H02","B2m",0.789333,0.846,0.120239,0.081265]
["H03","Hprt1",3.346,3.049333,0.137273,0.22194]
["H04","Ldha",-0.567333,-0.370667,0.018903,0.157662]
["H05","Rplp1",-0.710667,-0.587333,0.202231,0.421731]
["H06","RGDC",14.552667,14.002667,0.144143,0.671265]
["H07","RTC",3.356,3.152667,0.0799,0.625442]
["H08","RTC",3.286,2.936,0.081265,0.553285]
["H09","RTC",3.316,2.956,0.048,0.679149]
["H10","PPC",0.299333,0.192667,0.330632,0.828853]
["H11","PPC",0.339333,0.132667,0.178094,0.910702]
["H12","PPC",0.266,0.186,0.31312,0.886907]

{"sheet":"2^(-Avg.(Delta(Ct))","index":3,"n_rows":98,"n_cols":4,"rows_kept":98,"merged":[[0,0,1,0],[0,1,1,1],[0,2,0,3]],"flags":["formulas_cached_only"]}
["Position","Symbol","2^(-Avg.(Delta(Ct))"]
[null,null,"Control Group","Group 1"]
["A01","Apc",0.013175,0.012875]
["A02","Axin1",0.013735,0.012012]
["A03","Axin2",0.01859,0.018806]
["A04","Bcl9",0.004218,0.005314]
["A05","Btrc",0.012322,0.013054]
["A06","Vangl2",4.9e-05,6.1e-05]
["A07","Cby1",0.011523,0.01691]
["A08","Ccnd1",0.147011,0.192198]
["A09","Ccnd2",0.014961,0.024987]
["A10","Csnk1a1",0.117495,0.111414]
["A11","Csnk2a1",0.045986,0.050322]
["A12","Ctnnb1",0.129169,0.183095]
["B01","Daam1",0.078781,0.014518]
["B02","Dab2",0.069541,0.061811]
["B03","Dixdc1",0.007744,0.010876]
["B04","Dkk1",0.002051,0.002378]
["B05","Dkk3",0.569329,0.531202]
["B06","Dkk4",0.000516,0.000354]
["B07","Dvl1",0.021853,0.02747]
["B08","Dvl2",0.001115,0.00128]
["B09","Ep300",0.025103,0.02563]
["B10","Fbxw11",0.032819,0.037094]
["B11","Fgf4",4.2e-05,6.1e-05]
["B12","Fosl1",0.035256,0.032143]
["C01","Frzb",0.000849,0.001573]
["C02","Fzd1",0.078237,0.075224]
["C03","Fzd2",0.010171,0.014187]
["C04","Fzd3",0.001141,0.001325]
["C05","Fzd4",0.010628,0.012152]
["C06","Fzd5",0.012756,0.002173]
["C07","Fzd6",0.008814,0.009578]
["C08","Fzd7",0.00012,0.000135]
["C09","Fzd9",7.7e-05,6.1e-05]
["C10","Gsk3a",0.063109,0.069541]
["C11","Gsk3b",0.048161,0.060539]
["C12","Jun",0.034212,0.053934]
["D01","Kremen1",0.004867,0.006378]
["D02","Kremen2",6.1e-05,8e-05]
["D03","Lef1",0.000176,0.000201]
["D04","Lrp5",0.043006,0.048946]
["D05","Lrp6",0.152548,0.139725]
["D06","Mapk8",0.006006,0.004789]
["D07","Mitf",0.042023,0.054813]
["D08","Mmp7",4.2e-05,6.1e-05]
["D09","Myc",0.059567,0.066862]
["D10","Nkd1",0.006588,0.006422]
["D11","Nkd2",0.038314,0.019787]
["D12","Pitx2",0.00021,9e-05]
["E01","Porcn",0.018334,0.022572]
["E02","Ppard",0.021305,0.02353]
["E03","Prickle1",0.029784,0.029306]
["E04","Pygo2",0.014089,0.008875]
["E05","Rac1",0.221288,0.187808]
["E06","RGD1561440",0.003355,0.001558]
["E07","Rhoa",0.692875,0.790406]
["E08","Sfrp1",7e-05,6.1e-05]
["E09","Sfrp2",0.005501,0.004208]
["E10","Sfrp4",0.008794,0.012727]
["E11","Sfrp5",0.00036,0.000255]
["E12","Tcf7l1",0.007077,0.009848]
["F01","Tcf4",0.040592,0.053561]
["F02","Tcf7",0.000626,0.000651]
["F03","Tcf3",0.015961,0.014485]
["F04","Tle1",0.018249,0.017874]
["F05","Wif1",0.151494,0.009735]
["F06","Wisp1",0.027982,0.033741]
["F07","Wnt1",4.5e-05,6.1e-05]
["F08","Wnt10a",0.00015,6.1e-05]
["F09","Wnt10b",0.000301,0.000294]
["F10","Wnt11",0.003421,0.083853]
["F11","Wnt2",0.000122,6.1e-05]
["F12","Wnt2b",0.00056,0.000368]
["G01","Wnt3",4.2e-05,6.1e-05]
["G02","Wnt3a",4.4e-05,7.6e-05]
["G03","Wnt4",0.003196,0.00345]
["G04","Wnt5a",0.003294,0.003953]
["G05","Wnt5b",0.003621,0.00736]
["G06","Wnt6",0.000451,0.002941]
["G07","Wnt7a",4.2e-05,7.3e-05]
["G08","Wnt7b",0.000912,0.0006]
["G09","Wnt8a",0.000126,6.1e-05]
["G10","Wnt8b",4.2e-05,6.1e-05]
["G11","Wnt9a",0.003466,0.002439]
["G12","Wnt9b",9.3e-05,6.3e-05]
["H01","Actb",7.246746,7.659941]
["H02","B2m",0.578611,0.556325]
["H03","Hprt1",0.098345,0.120798]
["H04","Ldha",1.481782,1.29295]
["H05","Rplp1",1.63656,1.502467]
["H06","RGDC",4.2e-05,6.1e-05]
["H07","RTC",0.097666,0.112448]
["H08","RTC",0.102522,0.13067]
["H09","RTC",0.100412,0.128871]
["H10","PPC",0.812628,0.874987]
["H11","PPC",0.790406,0.912144]
["H12","PPC",0.831622,0.87904]

{"sheet":"Fold Change","index":4,"n_rows":140,"n_cols":5,"rows_kept":140,"merged":[[0,0,2,0],[0,1,2,1],[0,2,0,4],[1,2,1,4],[102,0,103,6],[104,0,105,4],[106,0,108,4],[109,0,111,4],[112,0,114,4],[115,0,117,4],[118,0,120,4],[123,0,124,4],[125,0,127,4],[128,0,130,4],[131,0,133,4],[134,0,136,4],[137,0,138,4]],"flags":["formulas_cached_only"]}
["Position","Symbol","Fold Change (comparing to control group)"]
[null,null,"Group 1"]
[null,null,"Fold Change","95% CI","Comments"]
["A01","Apc",0.9772,"( 0.82, 1.14 )"]
["A02","Axin1",0.8746,"( 0.64, 1.11 )"]
["A03","Axin2",1.0116,"( 0.83, 1.20 )"]
["A04","Bcl9",1.2599,"( 0.93, 1.59 )"]
["A05","Btrc",1.0595,"( 0.91, 1.21 )"]
["A06","Vangl2",1.234,"( 0.48, 1.99 )","B"]
["A07","Cby1",1.4675,"( 1.06, 1.88 )"]
["A08","Ccnd1",1.3074,"( 0.93, 1.69 )"]
["A09","Ccnd2",1.6702,"( 1.44, 1.90 )"]
["A10","Csnk1a1",0.9482,"( 0.73, 1.16 )"]
["A11","Csnk2a1",1.0943,"( 1.00, 1.18 )"]
["A12","Ctnnb1",1.4175,"( 1.16, 1.67 )"]
["B01","Daam1",0.1843,"( 0.00001, 0.50 )"]
["B02","Dab2",0.8888,"( 0.81, 0.97 )"]
["B03","Dixdc1",1.4044,"( 1.14, 1.67 )"]
["B04","Dkk1",1.1594,"( 0.91, 1.41 )"]
["B05","Dkk3",0.933,"( 0.77, 1.09 )"]
["B06","Dkk4",0.6846,"( 0.22, 1.15 )","B"]
["B07","Dvl1",1.257,"( 1.09, 1.43 )"]
["B08","Dvl2",1.1487,"( 0.64, 1.66 )","B"]
["B09","Ep300",1.021,"( 0.58, 1.46 )"]
["B10","Fbxw11",1.1303,"( 0.99, 1.27 )"]
["B11","Fgf4",1.4641,"( 0.68, 2.25 )","C"]
["B12","Fosl1",0.9117,"( 0.66, 1.16 )"]
["C01","Frzb",1.8532,"( 1.24, 2.47 )"]
["C02","Fzd1",0.9615,"( 0.76, 1.16 )"]
["C03","Fzd2",1.3947,"( 1.25, 1.54 )"]
["C04","Fzd3",1.162,"( 0.62, 1.70 )","B"]
["C05","Fzd4",1.1434,"( 0.88, 1.41 )"]
["C06","Fzd5",0.1704,"( 0.00001, 0.42 )"]
["C07","Fzd6",1.0867,"( 0.71, 1.47 )"]
["C08","Fzd7",1.1251,"( 0.84, 1.41 )","B"]
["C09","Fzd9",0.79,"( 0.30, 1.28 )","B"]
["C10","Gsk3a",1.1019,"( 1.00, 1.20 )"]
["C11","Gsk3b",1.257,"( 0.83, 1.68 )"]
["C12","Jun",1.5764,"( 1.36, 1.79 )"]
["D01","Kremen1",1.3104,"( 1.17, 1.45 )"]
["D02","Kremen2",1.3104,"( 0.68, 1.94 )","B"]
["D03","Lef1",1.1408,"( 0.00001, 2.60 )","B"]
["D04","Lrp5",1.1381,"( 0.97, 1.31 )"]
["D05","Lrp6",0.9159,"( 0.58, 1.25 )"]
["D06","Mapk8",0.7974,"( 0.63, 0.97 )"]
["D07","Mitf",1.3044,"( 0.86, 1.75 )"]
["D08","Mmp7",1.4641,"( 0.68, 2.25 )","C"]
["D09","Myc",1.1225,"( 0.91, 1.34 )"]
["D10","Nkd1",0.9749,"( 0.85, 1.10 )"]
["D11","Nkd2",0.5164,"( 0.42, 0.61 )"]
["D12","Pitx2",0.4293,"( 0.00001, 1.10 )","B"]
["E01","Porcn",1.2311,"( 0.63, 1.83 )"]
["E02","Ppard",1.1045,"( 1.01, 1.20 )"]
["E03","Prickle1",0.984,"( 0.86, 1.10 )"]
["E04","Pygo2",0.63,"( 0.20, 1.06 )"]
["E05","Rac1",0.8487,"( 0.60, 1.10 )"]
["E06","RGD1561440",0.4644,"( 0.20, 0.73 )","A"]
["E07","Rhoa",1.1408,"( 0.79, 1.49 )"]
["E08","Sfrp1",0.8685,"( 0.29, 1.44 )","B"]
["E09","Sfrp2",0.7649,"( 0.38, 1.15 )"]
["E10","Sfrp4",1.4473,"( 0.96, 1.94 )"]
["E11","Sfrp5",0.7087,"( 0.17, 1.25 )","B"]
["E12","Tcf7l1",1.3915,"( 1.27, 1.51 )"]
["F01","Tcf4",1.3195,"( 0.99, 1.65 )"]
["F02","Tcf7",1.0401,"( 0.67, 1.41 )","B"]
["F03","Tcf3",0.9075,"( 0.72, 1.10 )"]
["F04","Tle1",0.9794,"( 0.92, 1.04 )"]
["F05","Wif1",0.2003,"( 0.11, 0.29 )"]
["F06","Wisp1",1.2058,"( 0.85, 1.56 )"]
["F07","Wnt1",1.366,"( 0.62, 2.11 )","B"]
["F08","Wnt10a",0.4071,"( 0.00001, 0.96 )","B"]
["F09","Wnt10b",0.9772,"( 0.00001, 2.01 )","B"]
["F10","Wnt11",3.4852,"( 1.99, 4.98 )"]
["F11","Wnt2",0.4988,"( 0.00001, 1.05 )","B"]
["F12","Wnt2b",0.6567,"( 0.31, 1.01 )","B"]
["G01","Wnt3",1.4641,"( 0.68, 2.25 )","C"]
["G02","Wnt3a",1.7132,"( 0.07, 3.35 )","B"]
["G03","Wnt4",1.0792,"( 0.76, 1.40 )"]
["G04","Wnt5a",1.2002,"( 1.01, 1.39 )"]
["G05","Wnt5b",2.0326,"( 1.44, 2.63 )"]
["G06","Wnt6",2.6861,"( 1.39, 3.97 )"]
["G07","Wnt7a",1.7492,"( 0.73, 2.77 )","B"]
["G08","Wnt7b",0.6582,"( 0.20, 1.12 )","B"]
["G09","Wnt8a",0.483,"( 0.00001, 1.02 )","B"]
["G10","Wnt8b",1.4641,"( 0.68, 2.25 )","C"]
["G11","Wnt9a",0.7038,"( 0.52, 0.88 )"]
["G12","Wnt9b",0.6783,"( 0.00001, 1.40 )","B"]
["H01","Actb",1.057,"( 0.74, 1.38 )"]
["H02","B2m",0.9615,"( 0.85, 1.07 )"]
["H03","Hprt1",1.2283,"( 0.98, 1.48 )"]
["H04","Ldha",0.8726,"( 0.76, 0.98 )"]
["H05","Rplp1",0.9181,"( 0.58, 1.25 )"]
["H06","RGDC",1.4641,"( 0.68, 2.25 )","C"]
["H07","RTC",1.1514,"( 0.58, 1.72 )"]
["H08","RTC",1.2746,"( 0.72, 1.83 )"]
["H09","RTC",1.2834,"( 0.60, 1.97 )"]
["H10","PPC",1.0767,"( 0.32, 1.83 )"]
["H11","PPC",1.154,"( 0.31, 1.99 )"]
["H12","PPC",1.057,"( 0.28, 1.84 )"]
[]
[]
[]
["Comments: "]
[]
["A: This gene's average threshold cycle is relatively high (> 30) in either the control or the test sample, and is reasonably low in the other sample (< 30). "]
[]
["These data mean that the gene's expression is relatively low in one sample and reasonably detected in the other sample suggesting that the actual fold-change value is at least as large as the calculated and reported fold-change result. "]
[]
[]
["This fold-change result may also have greater variations if p value > 0.05; therefore, it is important to have a sufficient number of biological replicates to validate the result for this gene. "]
[]
[]
["B: This gene's average threshold cycle is relatively high (> 30), meaning that its relative expression level is low, in both control and test samples, and the p-value for the fold-change is either unavailable or relatively high (p > 0.05). "]
[]
[]
["This fold-change result may also have greater variations; therefore, it is important to have a sufficient number of biological replicates to validate the result for this gene. "]
[]
[]
["C: This gene's average threshold cycle is either not determined or greater than the defined cut-off (deault 35), in both samples meaning that its expression was undetected, making this fold-change result erroneous and un-interpretable. "]
[]
[]
[]
[]
["Fold Change & Fold Regulation: "]
[]
["Fold-Change (2^(- Delta Delta CT)) is the normalized gene expression (2^(- Delta CT)) in the Test Sample divided the normalized gene expression (2^(- Delta CT)) in the Control Sample. "]
[]
[]
["Fold-Regulation represents fold-change results in a biologically meaningful way. Fold-change values greater than one indicate a positive- or an up-regulation, and the fold-regulation is equal to the fold-change."]
[]
[]
["Fold-change values less than one indicate a negative or down-regulation, and the fold-regulation is the negative inverse of the fold-change. "]
[]
[]
["Fold-change and fold-regulation values greater than 2 are indicated in red; fold-change values less than 0.5 and fold-regulation values less than -2 are indicated in blue."]
[]
[]
["p-value:"]
[]
["The p values are calculated based on a Student's t-test of the replicate 2^(- Delta CT) values for each gene in the control group and treatment groups, and p values less than 0.05 are indicated in red. "]

{"sheet":"p-value","index":5,"n_rows":104,"n_cols":3,"rows_kept":104,"merged":[[0,0,1,0],[0,1,1,1],[0,2,0,2],[101,0,102,4],[103,0,107,4]],"flags":["formulas_cached_only"]}
["Position","Symbol","p-value (comparing to control group)"]
[null,null,"Group 1"]
["A01","Apc",0.837359]
["A02","Axin1",0.377334]
["A03","Axin2",0.865718]
["A04","Bcl9",0.146503]
["A05","Btrc",0.480497]
["A06","Vangl2",0.498356]
["A07","Cby1",0.033828]
["A08","Ccnd1",0.163261]
["A09","Ccnd2",0.00329]
["A10","Csnk1a1",0.70702]
["A11","Csnk2a1",0.108233]
["A12","Ctnnb1",0.012545]
["B01","Daam1",0.119661]
["B02","Dab2",0.060386]
["B03","Dixdc1",0.029673]
["B04","Dkk1",0.25985]
["B05","Dkk3",0.466137]
["B06","Dkk4",0.296998]
["B07","Dvl1",0.023099]
["B08","Dvl2",0.671248]
["B09","Ep300",0.781157]
["B10","Fbxw11",0.112109]
["B11","Fgf4",0.266375]
["B12","Fosl1",0.621656]
["C01","Frzb",0.023849]
["C02","Fzd1",0.795171]
["C03","Fzd2",0.004368]
["C04","Fzd3",0.487859]
["C05","Fzd4",0.354224]
["C06","Fzd5",0.106722]
["C07","Fzd6",0.593119]
["C08","Fzd7",0.418699]
["C09","Fzd9",0.571378]
["C10","Gsk3a",0.102536]
["C11","Gsk3b",0.226802]
["C12","Jun",0.003333]
["D01","Kremen1",0.005225]
["D02","Kremen2",0.281712]
["D03","Lef1",0.994311]
["D04","Lrp5",0.183925]
["D05","Lrp6",0.750658]
["D06","Mapk8",0.046754]
["D07","Mitf",0.194726]
["D08","Mmp7",0.266375]
["D09","Myc",0.285904]
["D10","Nkd1",0.710168]
["D11","Nkd2",0.001863]
["D12","Pitx2",0.205917]
["E01","Porcn",0.444736]
["E02","Ppard",0.093189]
["E03","Prickle1",0.827745]
["E04","Pygo2",0.237576]
["E05","Rac1",0.3509]
["E06","RGD1561440",0.067587]
["E07","Rhoa",0.432037]
["E08","Sfrp1",0.762666]
["E09","Sfrp2",0.440335]
["E10","Sfrp4",0.1178]
["E11","Sfrp5",0.460702]
["E12","Tcf7l1",0.002625]
["F01","Tcf4",0.114639]
["F02","Tcf7",0.789249]
["F03","Tcf3",0.378469]
["F04","Tle1",0.544691]
["F05","Wif1",0.030207]
["F06","Wisp1",0.263756]
["F07","Wnt1",0.325983]
["F08","Wnt10a",0.259683]
["F09","Wnt10b",0.996652]
["F10","Wnt11",0.043901]
["F11","Wnt2",0.239788]
["F12","Wnt2b",0.181468]
["G01","Wnt3",0.266375]
["G02","Wnt3a",0.349789]
["G03","Wnt4",0.649981]
["G04","Wnt5a",0.085694]
["G05","Wnt5b",0.016663]
["G06","Wnt6",0.036648]
["G07","Wnt7a",0.128013]
["G08","Wnt7b",0.260909]
["G09","Wnt8a",0.194391]
["G10","Wnt8b",0.266375]
["G11","Wnt9a",0.045206]
["G12","Wnt9b",0.409523]
["H01","Actb",0.654547]
["H02","B2m",0.531098]
["H03","Hprt1",0.138671]
["H04","Ldha",0.096725]
["H05","Rplp1",0.720992]
["H06","RGDC",0.266375]
["H07","RTC",0.518741]
["H08","RTC",0.349278]
["H09","RTC",0.383853]
["H10","PPC",0.690644]
["H11","PPC",0.563687]
["H12","PPC",0.705916]
[]
[]
[]
["p-value:"]
[]
["The p values are calculated based on a Student's t-test of the replicate 2^(- Delta CT) values for each gene in the control group and treatment groups, and p values less than 0.05 are indicated in red. "]

{"sheet":"Fold Regulation","index":6,"n_rows":140,"n_cols":4,"rows_kept":140,"merged":[[0,0,2,0],[0,1,2,1],[0,2,0,3],[1,2,1,3],[102,0,103,6],[104,0,105,4],[106,0,108,4],[109,0,111,4],[112,0,114,4],[115,0,117,4],[118,0,120,4],[123,0,124,4],[125,0,127,4],[128,0,130,4],[131,0,133,4],[134,0,136,4],[137,0,138,4],[139,0,142,4]],"flags":["formulas_cached_only"]}
["Position","Symbol","Up-Down Regulation (comparing to control group)"]
[null,null,"Group 1"]
[null,null,"Fold Regulation","Comments"]
["A01","Apc",-1.0234]
["A02","Axin1",-1.1434]
["A03","Axin2",1.0116]
["A04","Bcl9",1.2599]
["A05","Btrc",1.0595]
["A06","Vangl2",1.234,"B"]
["A07","Cby1",1.4675]
["A08","Ccnd1",1.3074]
["A09","Ccnd2",1.6702]
["A10","Csnk1a1",-1.0546]
["A11","Csnk2a1",1.0943]
["A12","Ctnnb1",1.4175]
["B01","Daam1",-5.4264]
["B02","Dab2",-1.1251]
["B03","Dixdc1",1.4044]
["B04","Dkk1",1.1594]
["B05","Dkk3",-1.0718]
["B06","Dkk4",-1.4607,"B"]
["B07","Dvl1",1.257]
["B08","Dvl2",1.1487,"B"]
["B09","Ep300",1.021]
["B10","Fbxw11",1.1303]
["B11","Fgf4",1.4641,"C"]
["B12","Fosl1",-1.0968]
["C01","Frzb",1.8532]
["C02","Fzd1",-1.0401]
["C03","Fzd2",1.3947]
["C04","Fzd3",1.162,"B"]
["C05","Fzd4",1.1434]
["C06","Fzd5",-5.8699]
["C07","Fzd6",1.0867]
["C08","Fzd7",1.1251,"B"]
["C09","Fzd9",-1.2658,"B"]
["C10","Gsk3a",1.1019]
["C11","Gsk3b",1.257]
["C12","Jun",1.5764]
["D01","Kremen1",1.3104]
["D02","Kremen2",1.3104,"B"]
["D03","Lef1",1.1408,"B"]
["D04","Lrp5",1.1381]
["D05","Lrp6",-1.0918]
["D06","Mapk8",-1.2541]
["D07","Mitf",1.3044]
["D08","Mmp7",1.4641,"C"]
["D09","Myc",1.1225]
["D10","Nkd1",-1.0257]
["D11","Nkd2",-1.9363]
["D12","Pitx2",-2.3295,"B"]
["E01","Porcn",1.2311]
["E02","Ppard",1.1045]
["E03","Prickle1",-1.0163]
["E04","Pygo2",-1.5874]
["E05","Rac1",-1.1783]
["E06","RGD1561440",-2.1535,"A"]
["E07","Rhoa",1.1408]
["E08","Sfrp1",-1.1514,"B"]
["E09","Sfrp2",-1.3074]
["E10","Sfrp4",1.4473]
["E11","Sfrp5",-1.4109,"B"]
["E12","Tcf7l1",1.3915]
["F01","Tcf4",1.3195]
["F02","Tcf7",1.0401,"B"]
["F03","Tcf3",-1.1019]
["F04","Tle1",-1.021]
["F05","Wif1",-15.5625]
["F06","Wisp1",1.2058]
["F07","Wnt1",1.366,"B"]
["F08","Wnt10a",-2.4566,"B"]
["F09","Wnt10b",-1.0234,"B"]
["F10","Wnt11",3.4852]
["F11","Wnt2",-2.0046,"B"]
["F12","Wnt2b",-1.5227,"B"]
["G01","Wnt3",1.4641,"C"]
["G02","Wnt3a",1.7132,"B"]
["G03","Wnt4",1.0792]
["G04","Wnt5a",1.2002]
["G05","Wnt5b",2.0326]
["G06","Wnt6",2.6861]
["G07","Wnt7a",1.7492,"B"]
["G08","Wnt7b",-1.5192,"B"]
["G09","Wnt8a",-2.0705,"B"]
["G10","Wnt8b",1.4641,"C"]
["G11","Wnt9a",-1.4208]
["G12","Wnt9b",-1.4743,"B"]
["H01","Actb",1.057]
["H02","B2m",-1.0401]
["H03","Hprt1",1.2283]
["H04","Ldha",-1.146]
["H05","Rplp1",-1.0892]
["H06","RGDC",1.4641,"C"]
["H07","RTC",1.1514]
["H08","RTC",1.2746]
["H09","RTC",1.2834]
["H10","PPC",1.0767]
["H11","PPC",1.154]
["H12","PPC",1.057]
[]
[]
[]
["Comments: "]
[]
["A: This gene's average threshold cycle is relatively high (> 30) in either the control or the test sample, and is reasonably low in the other sample (< 30). "]
[]
["These data mean that the gene's expression is relatively low in one sample and reasonably detected in the other sample suggesting that the actual fold-change value is at least as large as the calculated and reported fold-change result. "]
[]
[]
["This fold-change result may also have greater variations if p value > 0.05; therefore, it is important to have a sufficient number of biological replicates to validate the result for this gene. "]
[]
[]
["B: This gene's average threshold cycle is relatively high (> 30), meaning that its relative expression level is low, in both control and test samples, and the p-value for the fold-change is either unavailable or relatively high (p > 0.05). "]
[]
[]
["This fold-change result may also have greater variations; therefore, it is important to have a sufficient number of biological replicates to validate the result for this gene. "]
[]
[]
["C: This gene's average threshold cycle is either not determined or greater than the defined cut-off (deault 35), in both samples meaning that its expression was undetected, making this fold-change result erroneous and un-interpretable. "]
[]
[]
[]
[]
["Fold Change & Fold Regulation: "]
[]
["Fold-Change (2^(- Delta Delta CT)) is the normalized gene expression (2^(- Delta CT)) in the Test Sample divided the normalized gene expression (2^(- Delta CT)) in the Control Sample. "]
[]
[]
["Fold-Regulation represents fold-change results in a biologically meaningful way. Fold-change values greater than one indicate a positive- or an up-regulation, and the fold-regulation is equal to the fold-change. "]
[]
[]
["Fold-change values less than one indicate a negative or down-regulation, and the fold-regulation is the negative inverse of the fold-change. "]
[]
[]
["Fold-change and fold-regulation values greater than 2 are indicated in red; fold-change values less than 0.5 and fold-regulation values less than -2 are indicated in blue. "]
[]
[]
["p-value:"]
[]
["The p values are calculated based on a Student's t-test of the replicate 2^(- Delta CT) values for each gene in the control group and treatment groups, and p values less than 0.05 are indicated in red. "]