Movie EV8: Droplet formation at supersaturation conditions in drying p-tau441 solution on glass. Five microliter 50 ?M p-tau441 (50 mM NaCl, 20 mM HEPES pH7.4) were deposited as an open droplet on a glass surface and recorded at ambient conditions. Supersaturation of p-tau441 at the droplet border, as a consequence of evaporation, caused spontaneous droplet formation in absence of crowding agents. Imaging was done in brightfield with 40x Objective at a frame rate of 8/s-1.

